Flexible and powerful strategy for qualitative and quantitative analysis of valepotriates in Valeriana jatamansi Jones using high‐performance liquid chromatography with linear ion trap Orbitrap mass spectrometry

Abstract

Valeriana jatamansi Jones is an important medicinal plant and its quality is closely related to its region of origin. In the current study, we utilized a flexible and powerful strategy for comprehensive evaluation of the quality diversity for 15 regions in China. The method was based on a hybrid linear ion trap-Orbitrap mass spectrometry platform. For structure characterization, fragmentation patterns were detected by analyzing a series of standard compounds using data dependent multistage mass spectrometry acquisition. A fragment ion database for valepotriates was established, and the acquired data were high throughput filtered by fragment ion search for compound identification. For quantitative purposes, we normalized the mass spectrometry data of 15 samples using SIEVE 2.0 and the differences in composition were analyzed using principal component analysis combined with hierarchical clustering analysis. The results identified a total of 92 compounds from Valeriana jatamansi Jones. Samples from Dali, Kunming, and Baoshan have better qualities and concentrations of the main active constituents. To verify our strategy, we compared the valtrate, acevaltrate, and baldrinal contents using high-performance liquid chromatography with diode array detector. We developed and validated a comprehensive qualitative and quantitative analytical method to achieve quality control of Valeriana jatamansi Jones.

Microwave-Assisted Extraction of Multiple Trace Levels of Intermediate Metabolites for Camptothecin Biosynthesis in Camptotheca acuminata and Their Simultaneous Determination by HPLC-LTQ-Orbitrap-MS/MS and HPLC-TSQ-MS

“…Accurate mass measurements of the positive precursor and product ions masses were simultaneously performed, as shown in Figure S2. Seventeen putative metabolites belonging to iridoids of the CPT pathway were simultaneously annotated and identified based on their retention times, MS and MS/MS data compared with standards, if available, and with those previously published common standards, as shown in Table 2 and Figure S1A1–4 [28,29]. According to the backbone structure of compounds, among the 17 compounds identified, as shown in Figure S1B, 1 tryptamine, 2 iridoids or secoiridoids (loganic acid, secologanic acid), 2 compounds belonging to MIA strictosidinic acid, 3 compounds belonging to the vincosamide group (strictosamide, strictosamide epoxide, strictosamide diol), 8 compounds belonging to the pumiloside group (strictosamide ketolactam, pumiloside, deoxypumiloside), and 1 MIA camptothecin were included [12].…”

Suppression of the USP10/CCND1 axis induces glioblastoma cell apoptosis

“…To investigate, we screened the natural product library composed of ~1400 compounds. The cell viability assay showed that AVT, one of the most active valpotriate components derived from the herbal plant Valeriana jatamansi Jones [21], displayed potent GBM cell death-inducing activity in both cell lines (Fig. 6a).…”

DNA-Based Authentication and Metabolomics Analysis of Medicinal Plants Samples by DNA Barcoding and Ultra-High-Performance Liquid Chromatography/Triple Quadrupole Mass Spectrometry (UHPLC-MS)

“…HPLC standards were prepared at a concentration of 20 mg/L in methanol HPLC grade. Dilutions were prepared in a range of 0.05 to 1 mg/L in ethanol/water 70/30% (v/v) [52,53].…”