FleQ regulates both the type VI secretion system and flagella in <i>Pseudomonas putida</i>

Wang, Yuzhou; Li, Ye; Wang, Jianli; Wang, Xiaoyuan

doi:10.1002/bab.1611

Cited by 26 publications

(26 citation statements)

References 53 publications

(64 reference statements)

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“…FleQ is the most important transcription factor (TF) and master regulator of biofilm formation and motility responsive to cdGMP. The same TF is also related to other functions, including iron homeostasis (Blanco-Romero et al, 2018) and the control of Type 6 secretion system (Wang et al, 2018). In P. putida in conditions of low cdGMP, FleQ can interact with σ 54 to regulate a high number of promoters related to motility and flagella.…”

Section: Introductionmentioning

confidence: 99%

The Wsp intermembrane complex mediates metabolic control of the swim‐attach decision of Pseudomonas putida

Hueso-Gil

Calles

2020

Environmental Microbiology

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Summary Pseudomonas putida is a microorganism of biotechnological interest that—similar to many other environmental bacteria—adheres to surfaces and forms biofilms. Although various mechanisms contributing to the swim‐attach decision have been studied in this species, the role of a 7‐gene operon homologous to the wsp cluster of Pseudomonas aeruginosa—which regulates cyclic di‐GMP (cdGMP) levels upon surface contact—remained to be investigated. In this work, the function of the wsp operon of P. putida KT2440 has been characterized through inspection of single and multiple wsp deletion variants, complementation with Pseudomonas aeruginosa's homologues, combined with mutations of regulatory genes fleQ and fleN and removal of the flagellar regulator fglZ. The ability of the resulting strains to form biofilms at 6 and 24 h under three different carbon regimes (citrate, glucose and fructose) revealed that the Wsp complex delivers a similar function to both Pseudomonas species. In P. putida, the key components include WspR, a protein that harbours the domain for producing cdGMP, and WspF, which controls its activity. These results not only contribute to a deeper understanding of the network that regulates the sessile‐planktonic decision of P. putida but also suggest strategies to exogenously control such a lifestyle switch.

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Section: Introductionmentioning

confidence: 99%

The Wsp intermembrane complex mediates metabolic control of the swim‐attach decision of Pseudomonas putida

Hueso-Gil

Calles

2020

Environmental Microbiology

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“…In this regulatory cascade, the transcriptional regulator FleQ appears to be the master regulator 2 . The function of FleQ in flagella synthesis regulation has been studied in Pseudomonas aeruginosa 1 , 2 , in P. putida 3 – 5 and in P. fluorescens 6 – 8 . In these species, mutations in the fleQ gene result in non-motile, aflagellated bacteria.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of the FleQ direct regulon in Pseudomonas fluorescens F113 and Pseudomonas putida KT2440

Blanco-Romero

Redondo‐Nieto

Martínez‐Granero

et al. 2018

Sci Rep

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Bacterial motility plays a crucial role in competitiveness and colonization in the rhizosphere. In this work, Chromatin ImmunoPrecipitation Sequencing (ChIP-seq) analysis has been used to identify genes putatively regulated by the transcriptional regulatory protein FleQ in Pseudomonas fluorescens F113 and Pseudomonas putida KT2440. This protein was previously identified as a master regulator of flagella and biofilm formation in both strains. This work has demonstrated that FleQ from both bacteria are conserved and functionally equivalent for motility regulation. Furthermore, the ChIP-seq analysis has shown that FleQ is a global regulator with the identification of 121 and 103 FleQ putative binding sites in P. fluorescens F113 and P. putida KT2440 respectively. Putative genes regulated by FleQ included, as expected, flagellar and motility-related genes and others involved in adhesion and exopolysaccharide production. Surprisingly, the ChIP-seq analysis also identified iron homeostasis-related genes for which positive regulation was shown by RT-qPCR. The results also showed that FleQ from P. fluorescens F113 shares an important part of its direct regulon with AmrZ, a global regulator also implicated in environmental adaption. Although AmrZ also regulates motility and iron uptake, the overlap occurred mostly with the iron-related genes, since both regulators control a different set of motility-related genes.

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“…(1234567890) www.nature.com/scientificreports/ and it has been previously linked to the control of T6SS in P. aeruginosa 52 . FleQ belongs to the NtrC/NifA family and has been related to the regulation of T6SS and c-di-GMP metabolism in P. putida 89 . The differential gene expression analyses shown in Fig.…”

Section: Identification Of Eight Putative Type 6 Effectors In P Fluomentioning

confidence: 99%

Pseudomonas fluorescens F113 type VI secretion systems mediate bacterial killing and adaption to the rhizosphere microbiome

Durán

Bernal

Vazquez-Arias

et al. 2021

Sci Rep

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The genome of Pseudomonas fluorescens F113, a model rhizobacterium and a plant growth-promoting agent, encodes three putative type VI secretion systems (T6SSs); F1-, F2- and F3-T6SS. Bioinformatic analysis of the F113 T6SSs has revealed that they belong to group 3, group 1.1, and group 4a, respectively, similar to those previously described in Pseudomonas aeruginosa. In addition, in silico analyses allowed us to identify genes encoding a total of five orphan VgrG proteins and eight putative effectors (Tfe), some with their cognate immunity protein (Tfi) pairs. Genes encoding Tfe and Tfi are found in the proximity of P. fluorescens F113 vgrG, hcp, eagR and tap genes. RNA-Seq analyses in liquid culture and rhizosphere have revealed that F1- and F3-T6SS are expressed under all conditions, indicating that they are active systems, while F2-T6SS did not show any relevant expression under the tested conditions. The analysis of structural mutants in the three T6SSs has shown that the active F1- and F3-T6SSs are involved in interbacterial killing while F2 is not active in these conditions and its role is still unknown.. A rhizosphere colonization analysis of the double mutant affected in the F1- and F3-T6SS clusters showed that the double mutant was severely impaired in persistence in the rhizosphere microbiome, revealing the importance of these two systems for rhizosphere adaption.

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FleQ regulates both the type VI secretion system and flagella in Pseudomonas putida

Cited by 26 publications

References 53 publications

The Wsp intermembrane complex mediates metabolic control of the swim‐attach decision of Pseudomonas putida

The Wsp intermembrane complex mediates metabolic control of the swim‐attach decision of Pseudomonas putida

Genome-wide analysis of the FleQ direct regulon in Pseudomonas fluorescens F113 and Pseudomonas putida KT2440

Pseudomonas fluorescens F113 type VI secretion systems mediate bacterial killing and adaption to the rhizosphere microbiome

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