Flaxseed oil modulates semen production and its quality profiles, freezability, testicular biometrics and endocrinological profiles in mithun

Perumal, P.; Chang, Sukkum Ngullie; Khate, K.; Vüpru, Kezhavituo; Bag, Sadhan

doi:10.1016/j.theriogenology.2019.06.029

Cited by 28 publications

(26 citation statements)

References 61 publications

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“…Feeding male rabbits with fish oil resulted in the accumulation of n-3 LC PUFA in the testes and sperm. Flaxseed administration mainly increased ALA, but it also confirmed a certain LC PUFA synthesis and the ability of animals (liver and testis) to elongate and desaturate ALA [26,37]. In agreement with our previous research [33], we demonstrated the ability of the reproductive tissues (e.g., ovary) to efficiently synthesize and accumulate n-3 LC PUFA.…”

Section: Discussionsupporting

confidence: 91%

Effect of Dietaryn‐3Source on Rabbit Male Reproduction

Castellini

Mattioli

Signorini

et al. 2019

Oxidative Medicine and Cellular Longevity

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In the last two decades, the human sperm count linearly decreased in Western countries. Health problems, lifestyle, pollutants, and dietary behaviours are considered as the main risk factors, and the unbalance of dietary n‐6/n‐3 fatty acids is one of the most relevant. The aim of the present research is to study the effect of different dietary sources of n‐3 polyunsaturated fatty acids (PUFA) on reproductive traits using rabbit buck as the animal model. Fifteen rabbit bucks were assigned to three experimental groups: the control group, the FLAX group fed 10% extruded flaxseed, and the FISH group fed 3.5% fish oil for 110 days (50-day adaptation and 60-day experimental periods). Semen samples were collected weekly, whereas blood was collected every two weeks for the analytical determination of semen traits, oxidative status, fatty acid profiles, isoprostanes, neuroprostanes, and the immunocytochemistry of docosahexaenoic acid (DHA) and eicosapentaenoic (EPA) acid. At the end of the trial, the rabbits were killed and the testes were removed and stored for the analysis of fatty acid profile and immunocytochemistry. Results showed that dietary administration of n‐3 PUFA improved the track speed of the sperm and increased the n‐3 long-chain PUFA mainly confined in the sperm tail. Seminal plasma increased the thiobarbituric reactive substances (TBARs) by three times in the groups fed supplemental n‐3, whereas the F2-isoprotanes (F2-IsoPs) and F4-neuroprostanes (F4-NeuroPs) were lower and higher, respectively, in both supplemented groups than in the control. The testes and sperm showed a higher DHA and EPA distribution in rabbits from the n‐3 supplemented groups compared with the control. In conclusion, supplemental dietary n‐3 PUFA improved sperm motion traits and resulted in an enrichment of membrane fatty acid in the sperm and testes of the rabbits. However, such an increased amount of PUFA negatively affected the sperm oxidative status, which was mainly correlated with the generation of F4-NeuroPs with respect to F2-IsoPs. Accordingly, the latter cannot be considered a good marker of oxidation when diets rich in n‐3 PUFA are provided.

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Section: Discussionsupporting

confidence: 91%

Effect of Dietaryn‐3Source on Rabbit Male Reproduction

Castellini

Mattioli

Signorini

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…According to their origin, there are two types of antioxidants: Those produced synthetically, which have been used since 1995, and those formed naturally, antioxidants contained in vegetable oils, used recently to preserve better sperm quality. Many scientific reports have analyzed the effects of vegetable oils on semen quality, either as an animal diet supplementation [33][34][35][36][37][38][39][40] or as a cryoprotectant substance in sperm extenders [20][21][22][23][24][25][41][42][43][44].…”

Section: Discussionmentioning

confidence: 99%

Effects of Gossypium spp., Balanites aegyptiaca, and Sesamum indicum seeds oils on quality of chilled and frozen-thawed ram semen

Akourki

Arantxa²,

Perdomo³

et al. 2021

Vet World

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Background and Aim: Essential oils found frequently in plants are well known for their activities against bacteria, viruses, and fungi, and antioxidant properties. This study aimed to analyze egg yolk replacement by seed oils of Gossypium spp. (cotton), Balanites aegyptiaca (desert date), and Sesamum indicum (sesame) in semen extender, on ram sperm quality chilled at 4°C and frozen-thawed. Materials and Methods: Ejaculates were collected from adult rams and refrigerated at 4°C in a Tris-based extender containing 1.25%, 2.5%, 5%, and 10% of Gossypium spp., B. aegyptiaca, and S. indicum seed oils, to evaluate which were the two best extenders for comparison with BIOXcell, a commercial extender for deep freezing ram semen. Results: The data showed that sperm movements analyzed by the CASA system were faster in extenders supplemented with 2.5-5% of cottonseed oil and 1.25-10% of sesame oil, whereas in the extender containing B. aegyptiaca oil, all seminal parameters studied had the worst values. During the sperm-freezing process, 5% of cottonseed oil and 5% sesame seed oil were selected from the first study, with sesame oil reaching the best sperm quality. Thus, sperm motility and velocity were 44.14±13.99%, 24.44±12.6%, and 25.92±11.50%; and 20.26±9.56%, 8.76±6.38%, and 9.42±5.40%, respectively, for sesame oil, cottonseed oil, and BIOXcell. Conclusion: In summary, 2.5-10% of cottonseed oil and 1.25-10% of sesame seed oil can replace egg yolk in a Tris–egg yolk–based extender. Moreover, a Tris-based extender supplemented with 5% sesame seed oil could be an alternative for deep freezing ram semen, even though these results need to be confirmed with semen collected from rams with appropriate sexual rest.

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“…Flaxseed is a source of omega n-3 fatty acids containing about 52–58% of total fatty acids and lignans [ 29 ]. Moreover, flaxseed oil contains 58% α-linolenic acid, which is a vital antioxidant source that improves animal health not only reproductively [ 20 , 30 ] but also through improving inflammation and brain development [ 27 ]. Flaxseed oil is further comprised of stearic, oleic, linoleic, and palmitic acids, which contain a high content of vitamins including vitamin E [ 30 ].…”

Section: Flaxseed Oil Compositionmentioning

confidence: 99%

“…Molecular studies have shown the loss of proteins, lipids, and ions during cryopreservation to be a primary cause of poor post-thawed sperm quality [ 13 ]. Enriching diets with fatty acids such as omega n-3 has been shown to improve sperm motility [ 16 ], sperm membrane fluidity, and reproductive performance [ 17 ] in chickens [ 18 ], bulls [ 19 , 20 , 21 , 22 ], rams [ 23 ], and even in buffalos [ 17 ]. Ahmad et al [ 16 ], reported that the dietary inclusion of PUFA’s plays a vital role in improving fertilization rate through improving sperm membrane integrity.…”

Section: Introductionmentioning

confidence: 99%

Flaxseed Oil as a Source of Omega n-3 Fatty Acids to Improve Semen Quality from Livestock Animals: A Review

Ngcobo

Ramukhithi

Nephawe

et al. 2021

Animals

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The demand to conserve indigenous species through the cryo-gene bank is increasing. Spermatozoa remain sensitive to cryopreservation damages especially that of avian species thus limiting the use of reproductive biotechnologies such as artificial insemination in the conservation programs. Long-chain polyunsaturated fatty acid (LCPUFAs), specifically omega n-3, expanded a research interest to improve animal reproductive efficiency through improving spermatozoa quality. This is driven by the fact that mammals cannot synthesize omega-3 de-novo because they lack delta-12 and delta-15 desaturase enzymes thus supplemented in the diet is mandatory. Delta-12 and delta-15 add a double bond at the 12th and 15th carbon-carbon bond from the methyl end of fatty acids, lengthening the chain to 22 carbon molecules. Fish oil is a pioneer source of omega n-3 and n-6 fatty acids. However, there is a report that numerous fisheries are over-exploited and could collapse. Furthermore, processing techniques used for processing by-products could complement alterations of the amino acid profile and reduce protein retrieval. Alternatively, flaxseed oil contains ±52–58% of total fatty acids and lignans in the form of α-linolenic and linoleic acid. Alpha-linolenic acid (ALA,18:3n-3) is enzymatically broken-down de-novo by delta-6 desaturase and lengthened into a long-chain carbon molecule such as eicosapentaenoic acid (C20:5n-3). Nevertheless, controversial findings following the enrichment of diet with flaxseed oil have been reported. Therefore, this paper is aimed to postulate the role of flaxseed oil as an alternative source of omega n-3 and n-6 fatty acids to improve semen quality and quantity from livestock animals. These include the interaction between docosahexaenoic acid (DHA) and spermatogenesis, the interaction between docosahexaenoic acid (DHA) and testicular cells, and the effect of flaxseed oil on semen quality. It additionally assesses the antioxidants to balance the level of PUFAs in the semen.

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Flaxseed oil modulates semen production and its quality profiles, freezability, testicular biometrics and endocrinological profiles in mithun

Cited by 28 publications

References 61 publications

Effect of Dietaryn‐3Source on Rabbit Male Reproduction

Effect of Dietaryn‐3Source on Rabbit Male Reproduction

Effects of Gossypium spp., Balanites aegyptiaca, and Sesamum indicum seeds oils on quality of chilled and frozen-thawed ram semen

Flaxseed Oil as a Source of Omega n-3 Fatty Acids to Improve Semen Quality from Livestock Animals: A Review

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