Flax Breeding and Cultivar Registration in Canada

Booker, Helen

doi:10.1007/978-3-030-23964-0_3

Cited by 3 publications

(2 citation statements)

References 31 publications

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“…Fusarium wilt, caused by a soil-borne fungus Fusarium oxysporum f. sp. lini , is a devastating disease in flax because it persists in the soil for decades and, while mild infection can result in some yield losses due to yellowing and browning, heavy infection can lead to complete crop loss consequent to severe wilting (Booker 2019 ). Here, we performed a QTL analysis for Fusarium wilt resistance in flax using a population of more than 700 RILs derived from a cross between wilt resistant ‘Bison’ and wilt susceptible ‘Novelty’ using phenotypic data collected from five site-years and genome-wide SNP data.…”

Section: Discussionmentioning

confidence: 99%

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Fine-mapping of a major locus for Fusarium wilt resistance in flax (Linum usitatissimum L.)

Cloutier,

Edwards,

Zheng

et al. 2024

Theor Appl Genet

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Key message Fine-mapping of a locus on chromosome 1 of flax identified an S-lectin receptor-like kinase (SRLK) as the most likely candidate for a major Fusarium wilt resistance gene. Abstract Fusarium wilt, caused by the soil-borne fungal pathogen Fusarium oxysporum f. sp. lini, is a devastating disease in flax. Genetic resistance can counteract this disease and limit its spread. To map major genes for Fusarium wilt resistance, a recombinant inbred line population of more than 700 individuals derived from a cross between resistant cultivar ‘Bison’ and susceptible cultivar ‘Novelty’ was phenotyped in Fusarium wilt nurseries at two sites for two and three years, respectively. The population was genotyped with 4487 single nucleotide polymorphism (SNP) markers. Twenty-four QTLs were identified with IciMapping, 18 quantitative trait nucleotides with 3VmrMLM and 108 linkage disequilibrium blocks with RTM-GWAS. All models identified a major QTL on chromosome 1 that explained 20–48% of the genetic variance for Fusarium wilt resistance. The locus was estimated to span ~ 867 Kb but included a ~ 400 Kb unresolved region. Whole-genome sequencing of ‘CDC Bethune’, ‘Bison’ and ‘Novelty’ produced ~ 450 Kb continuous sequences of the locus. Annotation revealed 110 genes, of which six were considered candidate genes. Fine-mapping with 12 SNPs and 15 Kompetitive allele-specific PCR (KASP) markers narrowed down the interval to ~ 69 Kb, which comprised the candidate genes Lus10025882 and Lus10025891. The latter, a G-type S-lectin receptor-like kinase (SRLK) is the most likely resistance gene because it is the only polymorphic one. In addition, Fusarium wilt resistance genes previously isolated in tomato and Arabidopsis belonged to the SRLK class. The robust KASP markers can be used in marker-assisted breeding to select for this major Fusarium wilt resistance locus.

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Section: Discussionmentioning

confidence: 99%

“…The latter is the only one caused by a soil-borne pathogen. Once established in a soil, Fusarium wilt can survive for 20 years or more (Booker 2019 ). Crop rotation is an important control method to reduce the soil inoculum over time, but such agronomic practices have their limitations.…”

Section: Introductionmentioning

confidence: 99%