Flavonoids Regulate Lipid Droplets Biogenesis in <i>Drosophila melanogaster</i>

Fantin, Marianna; Garelli, Francesca; Napoli, Barbara; Forgiarini, Alessia; Gumeni, Sentiljana; Martin, Sara De; Montopoli, Monica; Vantaggiato, Chiara; Orso, Genny

doi:10.1177/1934578x19852430

Cited by 11 publications

(10 citation statements)

References 62 publications

(82 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nrf2 modulates the response to ER stress and autophagy and mediates the crosstalk between lipid metabolism and antioxidant defense ( Jiang et al, 2015 ). NAR, as other flavonoids, is involved in regulating metabolism homeostasis ( Fantin et al, 2019 ). Moreover, NAR binds to calcium channels with two-pore domains (TPC2) and to lysosomal wide conductance calcium channels (BKCa), altering the ionic homeostasis of lysosomes, increasing the concentration of Ca 2+ and H + ions ( Saponara et al, 2009 ; Pafumi et al, 2017 ).…”

Section: Therapeutic Strategiesmentioning

confidence: 99%

Hereditary Spastic Paraplegia and Future Therapeutic Directions: Beneficial Effects of Small Compounds Acting on Cellular Stress

et al. 2021

Self Cite

View full text Add to dashboard Cite

Hereditary spastic paraplegia (HSP) is a group of inherited neurodegenerative conditions that share a characteristic feature of degeneration of the longest axons within the corticospinal tract, which leads to progressive spasticity and weakness of the lower limbs. Mutations of over 70 genes produce defects in various biological pathways: axonal transport, lipid metabolism, endoplasmic reticulum (ER) shaping, mitochondrial function, and endosomal trafficking. HSPs suffer from an adequate therapeutic plan. Currently the treatments foreseen for patients affected by this pathology are physiotherapy, to maintain the outgoing tone, and muscle relaxant therapies for spasticity. Very few clinical studies have been conducted, and it’s urgent to implement preclinical animal studies devoted to pharmacological test and screening, to expand the rose of compounds potentially attractive for clinical trials. Small animal models, such as Drosophila melanogaster and zebrafish, have been generated, analyzed, and used as preclinical model for screening of compounds and their effects. In this work, we briefly described the role of HSP-linked proteins in the organization of ER endomembrane system and in the regulation of ER homeostasis and stress as a common pathological mechanism for these HSP forms. We then focused our attention on the pharmacodynamic and pharmacokinetic features of some recently identified molecules with antioxidant property, such as salubrinal, guanabenz, N-acetyl cysteine, methylene blue, rapamycin, and naringenin, and on their potential use in future clinical studies. Expanding the models and the pharmacological screening for HSP disease is necessary to give an opportunity to patients and clinicians to test new molecules.

show abstract

Section: Therapeutic Strategiesmentioning

confidence: 99%

Hereditary Spastic Paraplegia and Future Therapeutic Directions: Beneficial Effects of Small Compounds Acting on Cellular Stress

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…To our knowledge, the activity of these enzymes has not been related to Hr96 until now. In the same line, Hr96 modulates the Minotaur ( mino ) activity, a conserved glycerol-3-phosphate O-acyltransferase responsible for triglycerides synthesis and lipid droplets biogenesis (Fantin et al, 2019 ). It has been shown that when this enzyme is down-regulated as observed upon bacterial infection, there is a progressive loss of lipid energy stores (Dionne et al, 2006 ), meanwhile, its expression is increased in the face of starvation (Fujikawa et al, 2009 ) possibly promoting a mitochondrial adaptation toward lipid metabolism.…”

Section: Discussionmentioning

confidence: 99%

Filtering of Data-Driven Gene Regulatory Networks Using Drosophila melanogaster as a Case Study

et al. 2021

View full text Add to dashboard Cite

Gene Regulatory Networks (GRNs) allow the study of regulation of gene expression of whole genomes. Among the most relevant advantages of using networks to depict this key process, there is the visual representation of large amounts of information and the application of graph theory to generate new knowledge. Nonetheless, despite the many uses of GRNs, it is still difficult and expensive to assign Transcription Factors (TFs) to the regulation of specific genes. ChIP-Seq allows the determination of TF Binding Sites (TFBSs) over whole genomes, but it is still an expensive technique that can only be applied one TF at a time and requires replicates to reduce its noise. Once TFBSs are determined, the assignment of each TF and its binding sites to the regulation of specific genes is not trivial, and it is often performed by carrying out site-specific experiments that are unfeasible to perform in all possible binding sites. Here, we addressed these relevant issues with a two-step methodology using Drosophila melanogaster as a case study. First, our protocol starts by gathering all transcription factor binding sites (TFBSs) determined with ChIP-Seq experiments available at ENCODE and FlyBase. Then each TFBS is used to assign TFs to the regulation of likely target genes based on the TFBS proximity to the transcription start site of all genes. In the final step, to try to select the most likely regulatory TF from those previously assigned to each gene, we employ GENIE3, a random forest-based method, and more than 9,000 RNA-seq experiments from D. melanogaster. Following, we employed known TF protein-protein interactions to estimate the feasibility of regulatory events in our filtered networks. Finally, we show how known interactions between co-regulatory TFs of each gene increase after the second step of our approach, and thus, the consistency of the TF-gene assignment. Also, we employed our methodology to create a network centered on the Drosophila melanogaster gene Hr96 to demonstrate the role of this transcription factor on mitochondrial gene regulation.

show abstract

“…It interrupts the siphon membrane. It goes into the larval body through the siphon and devastates the siphon membrane by interfering with the phospholipidbinding so that the membrane ruptures (Fantin et al, 2019) . The breakage can obstruct absorption the oxygen.…”

Section: Discussionmentioning

confidence: 99%

Biolarvacide of Culex quinquefasciatus

Oktiansyah

Wicaksono

Armanda

et al. 2020

View full text Add to dashboard Cite

Biolarvicide is the larvicide derived from plants and relatively safe. The objective of this study was to determine the effect of pletekan leaf powder (Ruellia tuberosa), mimosa (Mimosa pudica), papaya (Carica papaya), and guava (Psidium guajava) on Culex quiquefasciatus larval mortality. The method was an experimental with a completely randomized design consisted of 5 treatments (0 gr, 0,25 gr, 0,5 gr, 0,75 gr, and 1 gr) and 5 repetitions for each plant. The data were analyzed by analysis of variance. Based on the results, leaf powder used in the study, namely guava leaves powder (1 gr), mimosa (1 gr), leaves of pletekan (1 gr), and leaves of papaya (1 gr) showed a very significant effect (P < 0,05) on Culex quiquefasciatus larval mortality. It can be concluded that the pletekan, mimosa, papaya, and guava had the potential as biolarvicide of Culex quinquefasciatus.

show abstract

Flavonoids Regulate Lipid Droplets Biogenesis in Drosophila melanogaster

Cited by 11 publications

References 62 publications

Hereditary Spastic Paraplegia and Future Therapeutic Directions: Beneficial Effects of Small Compounds Acting on Cellular Stress

Hereditary Spastic Paraplegia and Future Therapeutic Directions: Beneficial Effects of Small Compounds Acting on Cellular Stress

Filtering of Data-Driven Gene Regulatory Networks Using Drosophila melanogaster as a Case Study

Biolarvacide of Culex quinquefasciatus

Contact Info

Product

Resources

About