Flavonoids in Ageratum conyzoides L. Exert Potent Antitumor Effects on Human Cervical Adenocarcinoma HeLa Cells In Vitro and In Vivo

Zeyan, Lin; Lin, Yanyan; Shen, Jin-Xing; Jiang, Meijiao; Hou, Youming

doi:10.1155/2020/2696350

Cited by 13 publications

(9 citation statements)

References 35 publications

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“…Flavonoids are a type of plant secondary metabolite that are widely present in many minor grains, such as Tartary buckwheat, quinoa, and barley [1][2][3]. In addition to being diverse in variety, flavonoids also possess complex structure types, and they perform many bioactive functions, including antioxidation effects [4,5], antiproliferative effects [6], and antibacterial effects [7,8]. Flavonoids have been commonly used in bacteriostatic therapy and are considered to possess the potential to replace some antibiotics; quercetin, for example, similarly to existing antibiotics, has the potential to treat Pseudomonas aeruginosa infection and its use as an alternative may help to ease the selection pressure due to antibiotic overuse [9].…”

Section: Introductionmentioning

confidence: 99%

Biotransformation of Flavonoids Improves Antimicrobial and Anti-Breast Cancer Activities In Vitro

Hao

Wei

Wang

et al. 2021

Foods

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Coarse cereals are rich in flavonoids, which are bioactive substances with a wide range of functions. Biotransformation is considered an emerging approach to methylate flavonoids, displaying prominent regio- and stereoselectivity. In the current study, liquiritigenin, naringenin, and hesperidin flavonoids were biotransformed using O-methyltransferases that were heterologously expressed in Saccharomyces cerevisiae BJ5464-NpgA. Nuclear magnetic resonance (NMR) spectroscopy was used together with high-resolution mass spectroscopy analysis to determine the structures of the resulting methylated transformants, and their antimicrobial and antiproliferation activities were also characterized. Among the five methylated flavonoids obtained, 7-methoxy-liquiritigenin had the strongest inhibitory effect on Candida albicans SC5314 (C. albicans SC5314), Staphylococcus aureus ATCC6538 (S. aureus ATCC6538), and Escherichia coli ATCC25922 (E. coli ATCC25922), which increased 7.65-, 1.49-, and 0.54-fold in comparison to the values of their unmethylated counterparts at 200, 250, and 400 μM, respectively. The results suggest that 3’-methoxyhesperetin showed the best antiproliferative activity against MCF-7 cells with IC50 values of 10.45 ± 0.45 µM, which was an increase of more than 14.35-fold compared to that of hesperetin. These results indicate that methylation enhances the antimicrobial activities and antiproliferative effects of flavonoids. The current study provides an experimental basis for further research on flavonoids as well as flavonoid-containing crops in the development of antimicrobial and anti-breast cancer drugs in addition to supplementary and health foods. The biotransformation method is ideal, as it represents a means for the sustainable production of bioactive flavonoids.

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Section: Introductionmentioning

confidence: 99%

Biotransformation of Flavonoids Improves Antimicrobial and Anti-Breast Cancer Activities In Vitro

Hao

Wei

Wang

et al. 2021

Foods

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“…Several studies have indicated that MFN2 is a tumor suppressor; for instance, MFN2 expression is lower in lung ( 13 ), colorectal ( 10 ), gastric ( 11 ), and liver ( 14 ) cancer tissues than in normal tissues. In a recent study, MFN2 knockdown in MCF7 and A549 cells elevated the proliferation, colony formation ability, and invasion of cancer cells ( 15 ). Additionally, overexpression of MFN2 in urinary bladder carcinoma cell lines inhibited the proliferation of cancer cells by inducing cell cycle arrest and apoptosis ( 16 ).…”

Section: Introductionmentioning

confidence: 97%

Mitofusin-2 Promotes the Epithelial-Mesenchymal Transition-Induced Cervical Cancer Progression

Song

Kim

et al. 2021

Immune Netw

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High expression of mitofusin-2 (MFN2), a mitochondrial fusion protein, has been frequently associated with poor prognosis of patients with cervical cancer. Here, we aimed to identify the function of MFN2 in cervical cancer to understand its influence on disease prognosis. To this end, from cervical adenocarcinoma, we performed an MTT assay and quantitative RT-PCR (qRT-PCR) analysis to assess the effects of MFN2 on the proliferation and of HeLa cells. Then, colony-formation ability and tumorigenesis were evaluated using a tumor xenograft mouse model. The migration ability related to MFN2 was also measured using a wound healing assay. Consequently, epithelial-mesenchymal transition (EMT) of MFN2-knockdowned HeLa cells originating from adenocarcinoma. markers related to MFN2 were assessed by qRT-PCR. Clinical data were analyzed using cBioPortal and The Cancer Genome Atlas. We found that MFN2 knockdown reduced the proliferation, colony formation ability, migration, and in vivo tumorigenesis of HeLa cells. Primarily, migration of MFN2-knockdowned HeLa cells decreased through the suppression of EMT. Thus, we concluded that MFN2 facilitates cancer progression and in vivo tumorigenesis in HeLa cells. These findings suggest that MFN2 could be a novel target to regulate the EMT program and tumorigenic potential in HeLa cells and might serve as a therapeutic target for cervical cancer. Taken together, this study is expected to contribute to the treatment of patients with cervical cancer.

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“…Furthermore, investigations have also reported the presence of a vast variety of phytoconstituents in the herb’s essential oil, such as benzofurans (precocene I, precocene II, and ageratochromene dimer), coumarin, chromene, flavonoids (kaempferol, quercetin, quercetin-3-rhamnopiranoside), alkaloids (caffeic acid, echinatine, phytol, and pyrrolizidine alkaloids), sterols (stigmasterol, β-sitosterol, and friedeline), terpenes (α-pinene, β-pinene), and eugenol varying in their concentrations from one place to another. These secondary metabolites are claimed to have diverse medicinal properties, including radioactive, antidiabetic, antimicrobial, anti-inflammatory, antioxidant, anticancer, and wound healing properties and many more [ 15 , 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Ageratum conyzoides L. and Its Secondary Metabolites in the Management of Different Fungal Pathogens

et al. 2021

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Ageratum conyzoides L. (Family—Asteraceae) is an annual aromatic invasive herb, mainly distributed over the tropical and subtropical regions of the world. It owns a reputed history of indigenous remedial uses, including as a wound dressing, an antimicrobial, and mouthwash as well as in treatment of dysentery, diarrhea, skin diseases, etc. In this review, the core idea is to present the antifungal potential of the selected medicinal plant and its secondary metabolites against different fungal pathogens. Additionally, toxicological studies (safety profile) conducted on the amazing plant A. conyzoides L. are discussed for the possible clinical development of this medicinal herb. Articles available from 2000 to 2020 were reviewed in detail to exhibit recent appraisals of the antifungal properties of A. conyzoides. Efforts were aimed at delivering evidences for the medicinal application of A. conyzoides by using globally recognized scientific search engines and databases so that an efficient approach for filling the lacunae in the research and development of antifungal drugs can be adopted. After analyzing the literature, it can be reported that the selected medicinal plant effectively suppressed the growth of numerous fungal species, such as Aspergillus, Alternaria, Candida, Fusarium, Phytophthora, and Pythium, owing to the presence of various secondary metabolites, particularly chromenes, terpenoids, flavonoids and coumarins. The possible mechanism of action of different secondary metabolites of the plant against fungal pathogens is also discussed briefly. However, it was found that only a few studies have been performed to demonstrate the plant’s dosage and safety profile in humans. Considered all together, A. conyzoides extract and its constituents may act as a promising biosource for the development of effective antifungal formulations for clinical use. However, in order to establish safety and efficacy, additional scientific research is required to explore chronic toxicological effects of ageratum, to determine the probability of interactions when used with different herbs, and to identify safe dosage. The particulars presented here not only bridge this gap but also furnish future research strategies for the investigators in microbiology, ethno-pharmacology, and drug discovery.

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Flavonoids in Ageratum conyzoides L. Exert Potent Antitumor Effects on Human Cervical Adenocarcinoma HeLa Cells In Vitro and In Vivo

Cited by 13 publications

References 35 publications

Biotransformation of Flavonoids Improves Antimicrobial and Anti-Breast Cancer Activities In Vitro

Biotransformation of Flavonoids Improves Antimicrobial and Anti-Breast Cancer Activities In Vitro

Mitofusin-2 Promotes the Epithelial-Mesenchymal Transition-Induced Cervical Cancer Progression

Ageratum conyzoides L. and Its Secondary Metabolites in the Management of Different Fungal Pathogens

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