Flavonoids from whole plant of Euphorbia hirta and their evaluation against experimentally induced gastroesophageal reflux disease in rats

Abstract

Background:Euphorbia hirta possesses antibacterial, anti-inflammatory, galactogenic, antidiarrheal, antioxidant, hypoglycemic, antiasthmatic, antiamebic, antifungal, and antimalarial activities.Objective:The overall objective of the current study was the investigation of the whole plant extract of E. hirta and flavonoids from E. hirta on gastroesophageal reflux disease (GERD) in rats.Materials and Methods:The whole plant extract of E. hirta was characterized by analysis of flavonoids (HPLC: HPLC, UV, IR, MS and 1HNMR). GERD model was induced surgically in Wistar rats under pentobarbitone sodium anesthesia (50 mg/kg, i.p.) and the tissue esophagus and stomach were removed. The tissues were washed with physiological saline and were examined for GERD. The whole plant extract of E. hirta in doses of 50, 100, and 200 mg/kg were administered orally twice daily at 10:00 and 16:00 hours, respectively, for 5 days and kaempferol (100 mg/kg) or omeprazole (OMZ) in the dose of 30 mg/kg 1 hour prior to the induction of GERD. Control groups received suspension of 1% carboxymethyl cellulose in distilled water (10 mL/kg).Results:The levels of gastric wall mucus increased and of plasma histamine and H+, K+ ATPase significantly decreased in groups treated by both the plant extract and flavonoids. Both the plant extract and flavonoids reduced the lipid peroxidation and superoxide dismutase and increased the levels of catalase and reduced glutathione.Conclusions:The whole plant extract of E. hirta is attributed to its antisecretory, gastroprotective, and antioxidant potential as that of quercetin, rutin, kaempferol, and proton pump blocker (omeprazole) to treat GERD.SUMMARY The aqueous extract of whole plant of Euphorbia hirta revealed the presence of kaempferol (0.0256%), quercetin (0.0557%), and rutin (0.0151%), and the ethyl acetate fraction of whole plant of E. hirta possesses kaempferol (0.0487%), quercetin (0.0789%), and rutin (0.0184%).The levels of gastric wall mucus increased and of plasma histamine and H+-K+-ATPase significantly decreased in rats groups treated by both the whole plant extract of E. hirta and flavonoids.Both the whole plant extract of E. hirta and flavonoids reduced the lipid peroxidation and superoxide dismutase and increased the levels of catalase and reduced glutathione in rats groups. Abbreviation used: 1HNMR: Proton Nuclear Magnetic Resonance Spectroscopy, CAT: Catalase, EHAE: Aqueous extract of Euphorbia hirta, EHEF: Ethyl Acetate Fractions of Euphorbia hirta, GERD: Gastroesophageal reflux disease, GSH: Reduced Glutathione, HPLC: High performance liquid chromatography, IR: Infrared spectroscopy, LPO: Lipid Peroxidase, MDA: Malondialdehyde, MS: Mass Spectroscopy, OMZ: Omeprazole, ROS: Reactive Oxygen Species, SOD: Superoxide dismutase, TBHQ: tert-Butylhydroquinone, TLC: Thin Layer Chromatography, UV: Ultraviolet, UV: Ultraviolet–Visible Spectroscopy

Phytochemicals of Euphorbia hirta L. and Their Inhibitory Potential Against SARS-CoV-2 Main Protease

“…Clearly, lipids and PPPKs are among the most diverse groups of phytochemicals in E. hirta. These groups are also observed to be significantly more abundant in quantity than other phytochemical groups in E. hirta extracts (Sharma et al, 2014;Rao et al, 2017).…”

“…The extract of E. hirta is taken orally as an aqueous decoction for most of its folkloric uses. As its English common name suggests, the plant has been used for asthma and other respiratory difficulties (Ekpo and Pretorius, 2007;Ogunlesi et al, 2009;Rao et al, 2017). In addition, available studies conclusively suggest its potential against dengue (Guzman et al, 2016;Perera et al, 2018;Suganthi and Ravi, 2018); however, additional studies are required to validate the results (Perera et al, 2018).…”

Chemophenetic Significance of Anomalocalyx uleanus Metabolites Are Revealed by Dereplication Using Molecular Networking Tools

“…Flavonoids and O-glycosylated flavonoids are compounds present in several genera of Euphorbiaceae, such as Alchornea [ 21 ], Chrozophora [ 11 ], Cnidoscolus [ 22 ], Croton [ 23 ], Euphorbia [ 24 ], Macaranga [ 25 ], Phyllanthus [ 26 ], Jatropha [ 27 ], Ricinus [ 28 ], Pedilanthus [ 29 ], and others. Several flavonoids, such as apigenin ( 1 ), naringenin ( 2 ), kaempferol ( 3 ), catechin/epicatechin ( 5 ), and quercitrin ( 15 ), identified in A. uleanus are present in most of the genera mentioned above.…”

A Comparison of Bioactive Constituents and in Vitro Antioxidant Potential of Asthma Weed (Euphorbia Hirta), With Those of Other Antiasthmatic Plants Growing in Tabuk Region, Saudi Arabia

“…3). The substantial antioxidant activity of E. hirta could be due to the presence of sesquiterpene lactones and quercetin in flavonoids in the methanolic extracts (Williams et al, 2004;Gupta et al, 2017).…”