Flavonoids from Pueraria mirifica roots and quantitative analysis using HPLC

Cho, Jin‐Gyeong; Park, Hee Jung; Huh, Gyu-Won; Lee, Seung Su; Bang, Myun‐Ho; Choi, Kyoung-Sook; Oh, Chang-Hwan; Ko, Sung-Kwon; Cho, Soo-Yeul; Chai, Kap-Yong; Kim, Jin-Ho; Baek, Nam‐In

doi:10.1007/s10068-014-0248-4

Cited by 11 publications

(7 citation statements)

References 10 publications

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“…(3) e third mechanical SPLET is brie y described when avonoid is deprotonated to a ord a typical anion Flav-Oand the sequential electron transfer from this anion happens (Equation (6)). Proton a nity (PA) and the electron transfer enthalpy (ETE) are two conceptual parameters which correspond to deprotonation and electron transfer, respectively (Equations (7) and (8)). Journal of Chemistry…”

Section: Eoretical Parameters and Computational Procedurementioning

confidence: 99%

“…Using B3LYP functional with 6-311G(d) basis set for studied mediums gas and methanol, the current DFT (density functional theory) study will provide an insight into structural features, conformational analyses, and electronic properties of the selective isoflavones 1-2, in which the result intensively related to explaining their reactivity with free radicals. Ambocin (3) was isolated from the root of Pueraria miri ca, while its 6-methoxylation compound (4) had recently been identi ed as a new compound existing in Dalbergia sissoo stem bark [4,7]. To the best of our knowledge, there have been no speci c theoretically useful account reports on their glycosides 3-4. erefore, we also set out a computational work on 7-glycosylated compound (3) and 6-methoxylated-7-glycosylated compound (4), within the aim of nding the e ects of chemical structure on the antioxidant capacity.…”

Section: Introductionmentioning

confidence: 99%

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Isoflavones and Isoflavone Glycosides: Structural-Electronic Properties and Antioxidant Relations—A Case of DFT Study

Son

Van

2019

Journal of Chemistry

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Isoflavonoids and isoflavonoid glycosides have drawn much attention because of their antioxidant radical-scavenging capacity. Based on computational methods, we now present the antioxidant potential results of genistein (1), biochanin A (2), ambocin (3), and tectorigenin 7-O-[β-D-apiofuranosyl-(1-6)-β-D-glucopyranoside] (4). e optimized structures of the neutral and radical forms have been determined by the DFT-B3LYP method with the 6-311G(d) basis set. From the findings and thermodynamic point of view, the ring B system of isoflavones is considered as an active center in facilitating antioxidant reactions. Antioxidant activities are mostly driven by O-H bond dissociation enthalpy (BDE) following hydrogen atom transfer (HAT) mechanism. Antioxidant ability can be arranged in the following order: compounds (4) > (3) > (2) > (1). Of comprehensive structural analysis, flavonoids with 4′-methylation and 6-methoxylation, especially 7-glycosylation would claim responsibility for antioxidant enhancement.

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Section: Eoretical Parameters and Computational Procedurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isoflavones and Isoflavone Glycosides: Structural-Electronic Properties and Antioxidant Relations—A Case of DFT Study

Son

Van

2019

Journal of Chemistry

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“…To this end, various quantitative analysis approaches have been reported, which include the use of high‐performance liquid chromatography (HPLC), 13–15 HPLC tandem mass spectrometry, 16,17 and enzyme‐linked immunosorbent assays (ELISA) 12,18–21 . Immunoassays overcome the disadvantages of the HPLC method, since less amount of organic sorbent is required, and they offer the possibility of multisample handling and very specific and sensitive quantitative analysis because of the principle based on antigen–antibody reaction 22 .…”

Section: Introductionmentioning

confidence: 99%

Kwakhurin‐magnetic particles conjugates enable fast enzyme immunoassay for the detection of kwakhurin in Pueraria candollei

Sakamoto

Eto

Nuntawong

et al. 2020

Phytochemical Analysis

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Introduction: Kwakhurin (Kwa) is one of the unique isoflavonoids produced in Pueraria candollei var. mirifica (P. candollei), which has long been used as folk medicine for rejuvenation in Thailand. Recently, the use of P. candollei-derived products has widely spread among Japanese women for cosmetic purposes. Correspondingly, there has been an increase in the number of reports regarding possible health hazards caused by estrogenic activity inherent to the plant; thus, the need for a detailed evaluation of the phytoestrogen content of P. candollei-derived products has gained a sense of urgency in recent years. Objective: This study aims to develop a rapid enzyme immunoassay that can be applied to the quantitative analysis of Kwa in P. candollei and its derived products. Material and Method: A rapid and sensitive immunoassay was developed with a combination of Kwa-specific monoclonal antibody (MAb 11F) and Kwa-magnetic particles (MPs) conjugates, which increased the surface area of the solid phase, resulting in a decrease in the immunoreaction time. Result: This novel MPs-based enzyme immunoassay (MPs-EIA) was used to determine Kwa concentration in the range from 2.44 to 78.1 ng/mL with a limit of detection of 1.90 ng/mL. Validation analyses revealed that the proposed MPs-EIA protocol was sufficiently precise and accurate for effective quantitative analysis of Kwa in P. candollei and its derived products.

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“…White kwao krua is a phytoestrogens enriched plant. 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 Previous studies revealed that at least 17 chemical components containing estrogenic activity were found in its tuberous roots, leave and stem, but predominantly found in tuberous roots. 56 Among these components, they could be categorized into 3 major groups including isoflavonoids, coumestrans, and chromenes.…”

Section: Discussionmentioning

confidence: 99%

“…Previous chemical analysis revealed the presence of phytoestrogens, including isoflavonoids, coumestrans, and chromenes in this plant. 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 The phytoestrogens are safe to use for therapy and do not cause mutagenesis. In addition, White kwao krua has shown the beneficial potency of anti-carcinogenic effect by inhibiting hepatic cytochrome P450 in a rat model.…”

Section: Introductionmentioning

confidence: 99%

Daily White kwao krua dietary supplement alleviates LDL oxidative susceptibility, plasma LDL level and improves vasculature in a hypercholesterolemia rabbit model

Ratanachamnong

Phivthong-ngam

Namchaiw

2020

Journal of Traditional and Complementary Medicine

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Background and aim White kwao krua is an edible plant that grows in Southeast Asia. It is very rich in natural phytoestrogens. Previous clinical studies revealed that the use of White kwao krua as a hormone replacement therapy has beneficial effects on the lipid profile of menopause women. In this present study, we utilized the hypercholesterolemia rabbit model to demonstrate the effect of White kwao krua on the daily intake of high-fat diet. Experimental procedure We induced hypercholesterolemia in rabbits by feeding with high-fat diet (1% cholesterol-containing diet). The animals were maintained 12 weeks for the experimentation. The White kwao krua supplement was administered 100 mg/kg/day, and the effects were monitored comparing with Statins and turmeric. Blood was collected periodically to monitor the plasma cholesterol level and the oxidative susceptibility of isolated LDL-cholesterol. At the end of the experiment, the aorta was collected from the animal and performed endothelial-dependent relaxation and endothelial-independent relaxation assays. The relative ratio of intima to media layer was microscopically evaluated from hematoxylin/eosin-stained tissues. Results and conclusion We showed that the White kwao krua supplement reduced LDL-cholesterol about 40% compared with high-fat diet consumption alone. Administration of White kwao krua had significantly prolonged the susceptibility of LDL-cholesterol to oxidation. Besides, it led to the improvement of vascular function by recovering endothelium-dependent relaxation and alleviating vascular structure impairment induced by high-fat dietary intake. Together, we suggest that White kwao krua should be used as a dietary supplement to reduce the atherogenesis in high-fat dietary consumption. Section Dietary therapy/nutrients supplements. Taxonomy Inflammation, Disease.

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Flavonoids from Pueraria mirifica roots and quantitative analysis using HPLC

Cited by 11 publications

References 10 publications

Isoflavones and Isoflavone Glycosides: Structural-Electronic Properties and Antioxidant Relations—A Case of DFT Study

Isoflavones and Isoflavone Glycosides: Structural-Electronic Properties and Antioxidant Relations—A Case of DFT Study

Kwakhurin‐magnetic particles conjugates enable fast enzyme immunoassay for the detection of kwakhurin in Pueraria candollei

Daily White kwao krua dietary supplement alleviates LDL oxidative susceptibility, plasma LDL level and improves vasculature in a hypercholesterolemia rabbit model

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