FLAVONOIDS CONSTITUENT ANALYSIS AND CELL CYCLE INHIBITION ACTIVITY OF ETHYLACETATE EXTRACT OF Vernonia amygdalina Delile. LEAVES ON LUNG CANCER CELL LINE

Hasibuan, Poppy Anjelisa Zaitun; Munir, Delfitri; Pertiwi, D.; Satria, D.; Lubis, Muhammad Fauzan

doi:10.31788/rjc.2020.1345625

Cited by 10 publications

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“…This concentration elucidates that the potency of the samples results in a 50 % fatality rate of cells. A lower IC 50 value in samples would indicate a higher potential as an anticancer agent ( Paudel et al, 2019 , Hasibuan et al, 2020 ). In this study, the IC 50 of Eav and Gem can be shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Five compounds success in determining, which C 19 H 20 O 7 candidate, C 29 H 40 O 5 candidate, C 34 H 46 O 9 candidate, C 25 H 46 O 14 candidate, and C 54 H 78 O 9 candidate with retention time sequentially 8.96 min, 9.85 min, 10.52 min, 10.36 min, and 12.59 min. In a previous study, LC-MS/MS analysis of Eav identified that some of components, including diosmetin, 4-methyllumbelliferyl glucuronide, chlorogenic acid, 4-methylumbelliferone, scutellarin, rhoifolin, 7-hydroxycoumarine, apigetrin, apigenin, baicalin, luteolin, ingenol-3-angelate, and 4-methoxycinnamic acid ( Hasibuan et al, 2020 ). Meanwhile, our study reported Eav contains vernonioside D and vernonioside E and these compounds were shown activity to bond with epidermal growth factor and mammalian target of rapamycin proteins ( Hasibuan et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…The Hypersil GOLD aQ column, measuring 50 × 1 mm × 1.9 μm, was subjected to a flow rate of 40 μL/min during the analysis, which lasted for a duration of 70 min. The outcomes were scrutinized through the utilization of Compound Discoverer software, employing mzCloud ( Hasibuan et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

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The ethyl acetate extract of Vernonia amygdalina leaf ameliorates gemcitabine effect against migration and invasion of PANC-1 cells via down-regulation the VEGF, COX2, and RAS/MEK pathways

Hasibuan,

Keliat,

Lubis

et al. 2024

Saudi Pharmaceutical Journal

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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The ethyl acetate extract of Vernonia amygdalina leaf ameliorates gemcitabine effect against migration and invasion of PANC-1 cells via down-regulation the VEGF, COX2, and RAS/MEK pathways

Hasibuan,

Keliat,

Lubis

et al. 2024

Saudi Pharmaceutical Journal

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“…The dispersion of HepG2 cells treated with IC50 doses of LDE (20 µg/mL) and sorafenib (2 µg/mL) during a 24-h incubation period is shown in Figure 2. To examine the distribution of HepG2 cells accumulating throughout the cell cycle, we utilized an untreated cell HepG2 as a control cell [30]. HepG2 cells treated with 20 µg/mL accumulated in the S phase, increasing from 19.58 % in untreated cells to 22.51 % in treated cells.…”

Section: Cell Cycle Arrest Activitymentioning

confidence: 99%

Duku (Lansium domesticum) Leaves Extract Induces Cell Cycle Arrest and Apoptosis of HepG2 Cells via PI3K/Akt Pathways

Lubis

Hasibuan²,

Syahputra³

et al. 2022

Trends Sci

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Liver cancer is a type of malignant cancer that causes many deaths in the world. Efforts to find natural ingredients that can be used to treat liver cancer are very important. This study investigated the anticancer activity of ethanol extract of Duku (Lansium domesticum) leaves (LDE) through the cytotoxic activity, cell cycle inhibition mechanism and apoptosis influence through the PI3K/Akt pathways. Secondary metabolites of LDE were determined by common qualitative method. Cell cycle arrest was carried out by flow cytometer at inhibitory concentration 50 (IC50), while the apoptosis test was obtained using double staining method. The IC50 value was used to identifying the expression of PI3K/Akt genes. The analyzed results were proven LDE can induce HepG2 cell death which IC50 value of 19.93 ± 0.93 μg/mL (ethanol extract), 267.06 ± 3.24 μg/mL (ethyl acetate extract), and 216.47 ± 2.87 μg/mL (n-hexane extract). Cell cycle arrest activity in HepG2 cells which IC50 value of ethanol extract of LDE occurs in S phase. Observation of apoptosis occurrence was obtained getting apoptosis in cell line. PI3K/Akt genes expression of ethanol extract of LDE was obtained using RT-PCR method. The bands density of PI3K/Akt at IC50 value treatment of 0.55 ± 0.02; 0.25 ± 0.01. The LDE was determined to contain secondary metabolites which can inhibit the growth of HepG2 cells through PI3K/Akt pathways. HIGHLIGHTS The ethanolic extract of Lansium domesticum leaves have the best cytotoxic activity against HepG2 cells line compared to ethyl acetate extract and n-hexane extract The ethanolic extract of Lansium domesticum leaves have anticancer activity against HepG2 cells line through cell cycle arrest and apoptosis induce The ethanolic extract of Lansium domesticum leaves can inhibit the expressions of PI3K/Akt/mTOR genes using RT-PCR GRAPHICAL ABSTRACT

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“…The data which were absorbed from each well were converted to percentage of viable cells. The equation to determine viability of cells [25]:…”

Section: Abts Radical Scavenging Activitymentioning

confidence: 99%

Phytochemical Profile and Pharmacological Activity of Vernonia amygdalina Delile Stem Bark Extracts Using Different Solvent Extraction

Lubis

Hasibuan

Syahputra

et al. 2022

Open Access Maced J Med Sci

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BACKGROUND: Vernonia amygdalina is a native plant from Africa which is widely distributed to Asia, especially in Indonesia. Parts of V. amygdalina such as roots, leaves, and bark are used by the community as traditional medicines such as antidiabetic, antibacterial, and anticancer. AIM: This study aims to show the effect of solvents in the V. amygdalina stem bark extraction process on phytochemical’s content and their correlation with pharmacological activities. METHODS: V. amygdalina extract from stem bark in this study was obtained using the maceration method with different solvents. The extracts were investigated for total phenolic content (TPC) and total flavonoids content (TFC) using calorimetry assay. Principal Component Analysis (PCA) was used to grouping the extracts based on Fourier-transform infrared (FTIR) data. Antioxidant activity of the extracts was done using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethyl benzhothiazoline sulphonic acid) (ABTS) assay. While, the cytotoxic activity of the extracts was carried out using MTT assay on PANC-1 cell line. The correlation phytochemical content and pharmacology activities of extracts were analyzed using person correlation method. RESULTS: There were significantly different TPC and total flavonoid content of extracts (p < 0.05). The ethyl acetate extract was identified to have the highest TPC, TFC, DPPH, ABTS, and cytotoxic values of 3.61 ± 0.03 mg GAE/g dry powder, 25.12 ± 0.09 mg QE/g dry powder, 27.12 ± 0.65 μg/mL, 67.02 ± 0.23 μg/mL, and 33.83 ± 0.82 μg/mL, respectively (p < 0.05). This is supported by PCA analysis which shows that there are differences in extracts based on FTIR data and there is a strong correlation between TPC and TFC values with antioxidant and cytotoxic activities. CONCLUSIONS: This study report that each extract of V. amygdalina stem bark gives a distinct phytochemical profile (TPC, TFC, and FTIR spectrum) that contributes to the antioxidant activity and cytotoxic activity.

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FLAVONOIDS CONSTITUENT ANALYSIS AND CELL CYCLE INHIBITION ACTIVITY OF ETHYLACETATE EXTRACT OF Vernonia amygdalina Delile. LEAVES ON LUNG CANCER CELL LINE

Cited by 10 publications

References 0 publications

The ethyl acetate extract of Vernonia amygdalina leaf ameliorates gemcitabine effect against migration and invasion of PANC-1 cells via down-regulation the VEGF, COX2, and RAS/MEK pathways

The ethyl acetate extract of Vernonia amygdalina leaf ameliorates gemcitabine effect against migration and invasion of PANC-1 cells via down-regulation the VEGF, COX2, and RAS/MEK pathways

Duku (Lansium domesticum) Leaves Extract Induces Cell Cycle Arrest and Apoptosis of HepG2 Cells via PI3K/Akt Pathways

Phytochemical Profile and Pharmacological Activity of Vernonia amygdalina Delile Stem Bark Extracts Using Different Solvent Extraction

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