Flavonoids analysis ofViciaspecies of Narbonensis complex:V. kalakhensisKhatt., Maxt. & Bisby andV. eristalioidesMaxt

Abstract

-A qualitative and quantitative analysis of flavonoids has been carried out for first time in Vicia eristalioides Maxt. and in Vicia kalakhensis Khatt., Maxt. & Bisby. Free aglycones were consistently absent from both species while kaempferol derivatives were pre dominant in V. kalakhensis; a more complex mixture of flavonoid glycosides,(kaempferol and quercetin glycosides) was present in V. eristalioides. Therer was no evidence of flavones glycosides.The flavonoid patterns of V. kalakhensis and V. eristalioides were compared with that of V. narbonensis which is considered to be the ancestor of the Narbonensis complex. The results indicate that qualitative and quantitative flavonoid data may be used in the study of the organization and evolution of the Narbonensis complex.

Identification of complex, naturally occurring flavonoid glycosides in Vicia faba and Pisum sativum leaves by HPLC-DAD-ESI-MSn and the genotypic effect on their flavonoid profile

“…had kaempferol-3-O-rhamnogalactoside-7-O-rhamnoside as main kaempferol glycoside associated with low concentrations of the acylated kaempferol glycosides at all assuming a lower enzyme activity of the acyl-transferase (Tomas-Lorente et al 1989). An effect of the cultivar on the presence of different kaempferol glycosides in V. faba was also demonstrated for leaves of V. faba species without quantification (Perrino et al, 1989) and had also been observed for other Vicia species (Campeol et al, 2000;Campeol et al, 2003;Perrino et al, 1989).…”

“…Vicia species including Vicia kalakhensis, Vicia narbonensis, Vicia galilaea and Vicia eristalioides were shown to have diverse flavonol glycoside profiles (Campeol, Catalano, Cremonini, & Morelli, 2000;Campeol, Cioni, Flamini, Rossi, & Cremonini, 2003) The aim of the present study were to characterize the flavonoid profile in V. faba and P. sativum leaves in order to define the future potential of these legume leaves. The chemical structure of the flavonoid glycoside including glycosylation pattern and acylation pattern is important to reveal information on possible bioactive effects of the compounds.…”

Comparison of flavonoid contents and antioxidant activities of Vicia species

“…Six flavonoid aglycones (apigenin, myricetin, kaempferol, luteolin, quercetin and diosmetin) were present in the acid-hydrolysed leaf extracts of Vicia species (Webb and Harborne, 1991). Campeol et al (2000) reported the presence of kaempferol and quercetin in three Vicia species, and Perrino and Maruca (1989) identified the flavonoids apigenin, kaempferol and quercetin in Vicia species. In particular, kaempferol was the most abundant flavonoid aglycone in V. faba .…”

“…In conclusion, we found that the flavonoid patterns differed among the ten Vicia species (Table 2). The flavonoid patterns of Vicia species have been investigated using HPLC and UV spectrophotometry (Perrino and Maruca, 1989; Webb and Harborne, 1991; Campeol et al , 2000; Campeol et al , 2003). Those studies reported that flavonoid data are meaningful at the sectional level, and suggested that variations in glycosidic type present in Vicia flavonoids could be interesting from a chemotaxonomic perspective.…”

Karyological and molecular characterisation of subgenusVicia(Fabaceae)

“…In the nineties of the last century , karyological, cytological, cytophotometric and biochemical data on Vicia faba and on other species belonging to Vicia genus have been published by our team (De Pace et al 1991;Maggini et al 1991Maggini et al , 1995Cremonini 1992;Cremonini et al 1992aCremonini et al , 1992bCremonini et al , 1993Cremonini et al , 1995Cremonini et al , 1998aCremonini et al , 1998bFrediani et al 1992;Lipucci et al 1992;Venora et al 1999;Campeol et al 2000;Kotseruba et al 2000). Despite the great positive impact of the molecular phylogeny in recent years, the information on the chromosomal complement is still fundamental to assess the phyletic relationships and allow the processing of phylogenetic trees comparable to those obtained from molecular data.…”