Flavonoids and sesquiterpene lactones have a wide occurrence in Centaurea (Asteraceae) [1][2][3][4][5]. Plants of this genus are used to treat inflammations, cardiovascular ailments, microbial infections, and ulcers [6][7][8]. In continuation of our study on Centaurea species [9], we report here five flavonoid aglycones 1-5 from the endemic species Centaurea sulphurea Willd.[10].The air-dried powdered aerial parts of Centaurea sulphurea (1 kg) were extracted in a Soxhlet apparatus with chloroform. The residue (7.5 g) was column chromatographed on silica gel (70-200 mesh) eluted with cyclohexane and dichloromethane (fractions 1-90), then with dichloromethane/ethyl acetate (fractions 91-130), and finally with ethyl acetate/ methanol (fractions 131-166) with increasing polarity. Compounds 1-5 were found in fractions 1-90 and fractions 91-130. TLC purification on silica gel, eluted with dichloromethane-methanol (9:1), furnished compounds 1-5, which were identified by UV, 1 H NMR, 13 C NMR, and MS analysis [11,12], and all these data were in good agreement with the respective literature data [13][14][15][16].