Flavone cirsimarin impairs cell proliferation, migration, and invasion in MCF-7 cells grown in 2D and 3D models

Serpeloni, Juliana Mara; Oliveira, Larissa Cristina Bastos de; Fujiike, Andressa Y.; Tuttis, Katiuska; Ribeiro, Diego Luís; Camara, Marcos Bispo Pinheiro; Rocha, Cláudia Quintino da; Cólus, Ilce Mara de Syllos

doi:10.1016/j.tiv.2022.105416

Cited by 4 publications

(1 citation statement)

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“…The antitumor effect of BS was assessed in MCF-7 cells using the dyes fluorescein diacetate (FDA, 7.5 µg/mL), Hoeschst 33.342 (Ho, 4.0 µg/mL), and propidium iodide (PI, 1.0 µg/mL) (Sigma-Aldrich, USA) for analysis of apoptosis or necrosis, through the presence or absence of apoptotic bodies, respectively 20 . Cells were exposed to BS (1–10 µg/mL) for 72 h with subsequent staining with fluorochromes and counting 300 cells/treatment.…”

Section: Methodsmentioning

confidence: 99%

Production and characterization of rhamnolipids by Pseudomonas aeruginosa isolated in the Amazon region, and potential antiviral, antitumor, and antimicrobial activity

Cerqueira dos Santos,

Araújo Torquato,

de Alexandria Santos

et al. 2024

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Biosurfactants encompass structurally and chemically diverse molecules with surface active properties, and a broad industrial deployment, including pharmaceuticals. The interest is growing mainly for the low toxicity, biodegradability, and production from renewable sources. In this work, the optimized biosurfactant production by Pseudomonas aeruginosa BM02, isolated from the soil of a mining area in the Brazilian Amazon region was assessed, in addition to its antiviral, antitumor, and antimicrobial activities. The optimal conditions for biosurfactant production were determined using a factorial design, which showed the best yield (2.28 mg/mL) at 25 °C, pH 5, and 1% glycerol. The biosurfactant obtained was characterized as a mixture of rhamnolipids with virucidal properties against Herpes Simplex Virus, Coronavirus, and Respiratory Syncytial Virus, in addition to antimicrobial properties against Gram-positive bacteria (Staphylococcus aureus and Enterococcus faecium), at 50 µg/mL. The antitumor activity of BS (12.5 µg/mL) was also demonstrated, with potential selectivity in reducing the proliferation of breast tumor cells, after 1 min of exposure. These results demonstrate the importance of studying the interconnection between cultivation conditions and properties of industrially important compounds, such as rhamnolipid-type biosurfactant from P. aeruginosa BM02, a promising and sustainable alternative in the development of new antiviral, antitumor, and antimicrobial prototypes.

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Section: Methodsmentioning

confidence: 99%