Flavobacterium brevivitae sp. nov., isolated from river water

Abstract

A bacterial strain, designated TTM-43 T , was isolated from a water sample taken from the Caohu River in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain TTM-43T were Gram-stain-negative, strictly aerobic, motile by gliding, rod-shaped and surrounded by a thick capsule. The strain formed bright-yellow colonies. Growth occurred at 15-30 8C (optimum, 20 8C), at pH 5-9 (optimum, pH 6) and with 0-0.3 % NaCl (optimum growth in the absence of added NaCl). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TTM-43 T belonged to the genus Flavobacterium and was most closely related to Flavobacterium terrae R2A1-13 T , with 97.8 % sequence similarity. Strain TTM-43T contained iso-C 15 : 0 (24.9 %), iso-C 15 : 1 G (12.6 %), summed feature 9(iso-C 17 : 1 v9c and/or 10-methyl C 16 : 0 ; 12.2 %), iso-C 17 : 0 3-OH (10.9 %), iso-C 15 : 0 3-OH (7.3 %) and iso-C 16 : 0 (6.7 %) as the predominant fatty acids. The major isoprenoid quinone was MK-6. The polar lipid profile consisted of phosphatidylethanolamine and several uncharacterized aminophospholipids and phospholipids. The major polyamine was homospermidine. The genomic DNA G+C content of strain TTM-43 T was 31 mol%.DNA-DNA relatedness of strain TTM-43 T with respect to type strains of recognized species of the genus Flavobacterium was less than 70 %. On the basis of phylogenetic inference and phenotypic data, strain TTM-43 T should be assigned to a novel species, for which the name Flavobacterium brevivitae sp. nov. is presented. The type strain is TTM-43 T