Flavanols protect the yeast<i>Saccharomyces cerevisiae</i>against heating and freezing/thawing injury

Naparło, Katarzyna; Żyracka, Ewa; Bartosz, Grzegorz; Sadowska-Bartosz, Izabela

doi:10.1111/jam.14170

Cited by 5 publications

(3 citation statements)

References 28 publications

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“…Carotenoid related genes FLAVONOL SYNTHASE 1 ( FLS1 ), PHYTOENE SYNTHASE (PSY ), and CHALCONE SYNTHASE ( CHS ) are also highly expressed at various developmental stages ( Table 1 and Figure S2 ). All three genes have purported roles in combating biotic stress ( Dao et al., 2011 ; Havaux, 2014 ; Naparlo et al., 2019 ).…”

Section: Resultsmentioning

confidence: 99%

“…FLS1 is significantly upregulated in anthetic and post-anthetic nectaries ( Table 1 and Figure S2 ). Additionally, accumulation of flavanols have also been shown to increase survival of yeast by reducing oxidative stress ( Naparlo et al., 2019 ). Thus, flavonoid accumulation may be a way to inhibit bacterial biofilms while simultaneously supporting symbiotic yeast.…”

Section: Discussionmentioning

confidence: 99%

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Nectary development in Cleome violacea

Carey

Zenchyzen²,

Deneka³

et al. 2023

Front. Plant Sci.

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Nectaries are a promising frontier for plant evo-devo research, and are particularly fascinating given their diversity in form, position, and secretion methods across angiosperms. Emerging model systems permit investigations of the molecular basis for nectary development and nectar secretion across a range of taxa, which addresses fundamental questions about underlying parallelisms and convergence. Herein, we explore nectary development and nectar secretion in the emerging model taxa, Cleome violacea (Cleomaceae), which exhibits a prominent adaxial nectary. First, we characterized nectary anatomy and quantified nectar secretion to establish a foundation for quantitative and functional gene experiments. Next, we leveraged RNA-seq to establish gene expression profiles of nectaries across three key stages of development: pre-anthesis, anthesis, and post-fertilization. We then performed functional studies on five genes that were putatively involved in nectary and nectar formation: CvCRABSCLAW (CvCRC), CvAGAMOUS (CvAG), CvSHATTERPROOF (CvSHP), CvSWEET9, and a highly expressed but uncharacterized transcript. These experiments revealed a high degree of functional convergence to homologues from other core Eudicots, especially Arabidopsis. CvCRC, redundantly with CvAG and CvSHP, are required for nectary initiation. Concordantly, CvSWEET9 is essential for nectar formation and secretion, which indicates that the process is eccrine based in C. violacea. While demonstration of conservation is informative to our understanding of nectary evolution, questions remain. For example, it is unknown which genes are downstream of the developmental initiators CvCRC, CvAG, and CvSHP, or what role the TCP gene family plays in nectary initiation in this family. Further to this, we have initiated a characterization of associations between nectaries, yeast, and bacteria, but more research is required beyond establishing their presence. Cleome violacea is an excellent model for continued research into nectary development because of its conspicuous nectaries, short generation time, and close taxonomic distance to Arabidopsis.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Nectary development in Cleome violacea

Carey

Zenchyzen²,

Deneka³

et al. 2023

Front. Plant Sci.

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“…The effects of catechins are critically dependent on their cellular action. We studied the protective effect of (+)-catechin and (−)-epigallocatechin gallate on the yeast Saccharomyces cerevisiae with respect to cellular mortality dependent on oxidative stress [17]. There are reports on the effects of catechins on erythrocytes.…”

Section: Introductionmentioning

confidence: 99%

Interaction of Catechins with Human Erythrocytes

et al. 2020

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The aim of this study was to characterize the interaction of chosen catechins ((+)-catechin, (−)-epigallocatechin (EGC), and (−)-epigallocatechin gallate (EGCG)) with human erythrocytes and their protective effects against oxidative damage of erythrocytes. Uptake of the catechins by erythrocytes was studied by fluorimetry, their interaction with erythrocyte membrane was probed by changes in erythrocyte osmotic fragility and in membrane fluidity evaluated with spin labels, while protection against oxidative damage was assessed by protection against hemolysis induced by permanganate and protection of erythrocyte membranes against lipid peroxidation and protein thiol group oxidation. Catechin uptake was similar for all the compounds studied. Accumulation of catechins in the erythrocyte membrane was demonstrated by the catechin-induced increase in osmotic resistance and rigidification of the erythrocyte membrane detected by spin labels 5-doxyl stearic acid and 16-doxyl stearic acid. (−)-Epigallocatechin and EGCG inhibited erythrocyte acetylcholinesterase (mixed-type inhibition). Catechins protected erythrocytes against permanganate-induced hemolysis, oxidation of erythrocyte protein thiol groups, as well as membrane lipid peroxidation. These results contribute to the knowledge of the beneficial effects of catechins present in plant-derived food and beverages.

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Disruption of YCP4 enhances freeze-thaw tolerance in Saccharomyces cerevisiae

Kim

2022

Biotechnol Lett

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Objective The aim of this study was to identify genes related to a freeze-thaw tolerance and to elucidate the tolerance mechanism in yeast Saccharomyces cerevisiae as an appropriate eukaryote model. ResultsIn this study, one tolerant strain under exposure to freeze-thaw stress was isolated by screening a transposon-mediated mutant library and the disrupted gene was identi ed to be YCP4. In addition, this phenotype related to freeze-thaw tolerance was com rmed by deletion and overexpressing of this corresponding gene. This mutant strain showed a freeze-thaw tolerance by the reduction in the intracellular level of reactive oxygen species (ROS) and the activation of the MSN2/4 and STRE-mediated genes such as CTT1 and HSP12.Conclusions Disruption of YCP4 in S. cerevisiae results in increased tolerance to freeze-thaw stress.

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Flavanols protect the yeastSaccharomyces cerevisiaeagainst heating and freezing/thawing injury

Cited by 5 publications

References 28 publications

Nectary development in Cleome violacea

Nectary development in Cleome violacea

Interaction of Catechins with Human Erythrocytes

Disruption of YCP4 enhances freeze-thaw tolerance in Saccharomyces cerevisiae

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