Flavanol production by Fagopyrum esculentum hairy and normal root cultures

Trotin, F; Moumou, Y.; Vasseur, J.

doi:10.1016/0031-9422(93)85231-f

Cited by 35 publications

(6 citation statements)

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“…Similarly, the levels of anthocyanins, which have antioxidant, anti-inflammatory, antiviral, and antihistamine properties, could be enhanced in tartary buckwheat ( Rice-Evans and Packer, 2003 ). Trotin et al (1993) and Lee et al (2007) developed buckwheat hairy root cultures to enhance the production of phenolic compounds, such as rutin, chlorogenic acid, and caffeic acid. Overall, R1000 has been recommended as a promising strain for the hairy root induction and for the phenolic compound accumulation in buckwheat.…”

Section: Discussionmentioning

confidence: 99%

Effect of Different Agrobacterium rhizogenes Strains on Hairy Root Induction and Phenylpropanoid Biosynthesis in Tartary Buckwheat (Fagopyrum tataricum Gaertn)

Thwe

Arasu

et al. 2016

Front. Microbiol.

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The development of an efficient protocol for successful hairy root induction by Agrobacterium rhizogenes is the key step toward an in vitro culturing method for the mass production of secondary metabolites. The selection of an effective Agrobacterium strain for the production of hairy roots is highly plant species dependent and must be determined empirically. Therefore, our goal was to investigate the transformation efficiency of different A. rhizogenes strains for the induction of transgenic hairy roots in Fagopyrum tataricum ‘Hokkai T10’ cultivar; to determine the expression levels of the polypropanoid biosynthetic pathway genes, such as ftpAL, FtC4H, Ft4CL, FrCHS, FrCH1, FrF3H, FtFLS1, FtFLS2, FtF3, H1, FtF3′H2, FtANS, and FtDFR; and to quantify the in vitro synthesis of phenolic compounds and anthocyanins. Among different strains, R1000 was the most promising candidate for hairy root stimulation because it induced the highest growth rate, root number, root length, transformation efficiency, and total anthocyanin and rutin content. The R1000, 15834, and A4 strains provided higher transcript levels for most metabolic pathway genes for the synthesis of rutin (22.31, 15.48, and 13.04 μg/mg DW, respectively), cyanidin 3-O-glucoside (800, 750, and 650 μg/g DW, respectively), and cyanidin 3-O-rutinoside (2410, 1530, and 1170 μg/g DW, respectively). A suitable A. rhizogenes strain could play a vital role in the fast growth of the bulk amount of hairy roots and secondary metabolites. Overall, R1000 was the most promising strain for hairy root induction in buckwheat.

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Section: Discussionmentioning

confidence: 99%

Effect of Different Agrobacterium rhizogenes Strains on Hairy Root Induction and Phenylpropanoid Biosynthesis in Tartary Buckwheat (Fagopyrum tataricum Gaertn)

Thwe

Arasu

et al. 2016

Front. Microbiol.

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“…Hairy root cultures of Fagopyrum esculentum established by infection with Agrobacterium rhizogenes strain 15834 produced five flavanols obtained: (+)-catechin, (-)-epicatechin, (-)epicatechin-3-0-gallate, procyanidin B2 and procyanidin BZ3'-0-gallate, also present in normal root cultures with a common prominency of epicatechin-3-0-gallate synthesis and inversion in the contents rates of (+)-catechin and (-) tepicatechin. The highest content of procyanidin B2-3'-0gallate was obtained in hairy root cultures which can be considered as a better production source for flavanols, especially of the two galloylated derivatives (Trotin et al, 1993). Matkowski (2008) found that antioxidants can be produced in vitro from different plants.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and antibacterial activity of callus and adventitious root extracts from Rumex vesicarius L.

A¹,

Mostafa²,

Eman³

2014

J. Med. Plants Res.

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The present work has been carried out for the in vitro production of biologically active constituents from Rumex vesicarius L. cultures. Calli and adventitious roots were tested for their antioxidant and antibacterial activities. Results of preliminary phytochemical screening revealed that cultures were rich in flavonoids, alkaloids, tannins, carbohydrates and/or glycosides, coumarins and cardiac glycosides. Sterols and/or triterpenodis, chlorides and sulphates were found in some cultures only. Calli and roots in all treatments were devoid of saponins, anthraquinones, irodoids and sublimable substances. Total phenolic content in roots produced by culture of leaf blade explant on media containing 1 mg/L kinetin (K) + 4 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D) and calli produced on media containing 2 mg/L K + 2mg/L 2,4-D produced high total phenolic content (21.48 ± 0.24 and 18.15 ± 0.238 mg/explants, respectively). Total flavonoid content of calli and roots produced on media containing 2 mg/L K + 2mg/L 2,4-D and 1 mg/L K + 4 mg/L 2,4-D produced high total flavonoid contents (52.82 ± 0.56 and 49.27 ± 0.56 µg/explant, respectively). Results of high performance liquid chromatography (HPLC) analysis of flavonoids of calli obtained on 2 mg/L K + 2mg/L 2,4-D showed high concentration of quercetin (7.35 ± 0.245 µg/g D.W.). High total antioxidant activity was found in the extract of calli obtained on 2 mg/L K + 2 mg/L 2,4-D (617.85 ± 20.11 GAE s , ppm). Highest 2,2-diphenylpicrylhydrazyl (DPPH) scavenging activity was obtained using adventitious roots and calli extracts (IC 50 = 1.57 ± 0.02 and 1.76 ± 0.021 mg/ml, respectively). Antibacterial activity of adventitious roots and calli extracts was negative against all 6 human pathogenic isolates investigated.

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“…Therefore, rhizosphere processes are the key to improved plant nutrition and increased crop yields (). In the case of biotechnology applications, root exudates can be utilized in the human diet (e.g., as food additives), because some of them are important antioxidants ( , ).…”

Section: Introductionmentioning

confidence: 99%

Exudation of Allelopathic Substances in Buckwheat (Fagopyrum esculentum Moench)

Kalinová

Vrchotová

Triska

2007

J. Agric. Food Chem.

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Root exudates of the common buckwheat, especially phenolic compounds, were studied. Their contents, both in the soil during the growing season and in agar medium during germination, were determined by HPLC and GC-MS. The allelopathic activity of the soil from a buckwheat stand was evaluated, as well. Palmitic acid, squalene, epicatechin, vitexin, a gallic acid derivative, and a quercetin derivative were the main compounds of the agar medium. In the soil, palmitic acid methyl ester, vanillic acid, rutin, a gallic acid derivative, and a 4-hydroxyacetophenone derivative were identified. The effects of vitexin, squalene, epicatechin, 4-hydroxyacetophenone, and vanillic and gallic acids were tested on eight plant species. Inhibitive effects were observed in the cases of 4-hydroxyacetophenone and vanillic and gallic acids. Comparisons of the identified compounds and inhibitive effects of soil extracts indicated that palmitic acid and the gallic acid derivative probably have an important function in the allelopathic root response of buckwheat.

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Flavanol production by Fagopyrum esculentum hairy and normal root cultures

Cited by 35 publications

References 9 publications

Effect of Different Agrobacterium rhizogenes Strains on Hairy Root Induction and Phenylpropanoid Biosynthesis in Tartary Buckwheat (Fagopyrum tataricum Gaertn)

Effect of Different Agrobacterium rhizogenes Strains on Hairy Root Induction and Phenylpropanoid Biosynthesis in Tartary Buckwheat (Fagopyrum tataricum Gaertn)

Antioxidant and antibacterial activity of callus and adventitious root extracts from Rumex vesicarius L.

Exudation of Allelopathic Substances in Buckwheat (Fagopyrum esculentum Moench)

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