Flat-panel volumetric computed tomography in cerebral perfusion: Evaluation of three rat stroke models

Juenemann, Martin; Goegel, Sinja; Obert, Martin; Schleicher, Nadine; Ritschel, Nouha; Doenges, Simone; Eitenmueller, Inka; Schwarz, Niko; Kastaun, Sabrina; Yeniguen, Mesut; Tschernatsch, Marlene; Gerriets, Tibo

doi:10.1016/j.jneumeth.2013.07.010

Cited by 2 publications

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“…We used fpVCT for noninvasive dynamic imaging of cerebral perfusion after temporary MCAO in the cortical and subcortical regions of the infarct and the whole hemisphere [32]. Without craniectomy, the EPO pretreatment led to a significant increase in CBF in the cortical regions of the ischemic tissue.…”

Section: Discussionmentioning

confidence: 99%

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Neuroprotective mechanisms of erythropoietin in a rat stroke model

Juenemann

Braun

Schleicher

et al. 2020

Translational Neuroscience

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AbstractObjectiveThis study was designed to investigate the indirect neuroprotective properties of recombinant human erythropoietin (rhEPO) pretreatment in a rat model of transient middle cerebral artery occlusion (MCAO).MethodsOne hundred and ten male Wistar rats were randomly assigned to four groups receiving either 5,000 IU/kg rhEPO intravenously or saline 15 minutes prior to MCAO and bilateral craniectomy or sham craniectomy. Bilateral craniectomy aimed at elimination of the space-consuming effect of postischemic edema. Diagnostic workup included neurological examination, assessment of infarct size and cerebral edema by magnetic resonance imaging, wet–dry technique, and quantification of hemispheric and local cerebral blood flow (CBF) by flat-panel volumetric computed tomography.ResultsIn the absence of craniectomy, EPO pretreatment led to a significant reduction in infarct volume (34.83 ± 9.84% vs. 25.28 ± 7.03%; p = 0.022) and midline shift (0.114 ± 0.023 cm vs. 0.083 ± 0.027 cm; p = 0.013). We observed a significant increase in regional CBF in cortical areas of the ischemic infarct (72.29 ± 24.00% vs. 105.53 ± 33.10%; p = 0.043) but not the whole hemispheres. Infarct size-independent parameters could not demonstrate a statistically significant reduction in cerebral edema with EPO treatment.ConclusionsSingle-dose pretreatment with rhEPO 5,000 IU/kg significantly reduces ischemic lesion volume and increases local CBF in penumbral areas of ischemia 24 h after transient MCAO in rats. Data suggest indirect neuroprotection from edema and the resultant pressure-reducing and blood flow-increasing effects mediated by EPO.

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Section: Discussionmentioning

confidence: 99%

“…The system is described in detail in the study by Obert et al (2010) [31]. Preparation and anesthesia of the rats, image acquisition, reconstruction, and analysis followed a previously published protocol [32].…”

Section: Fpvctmentioning

confidence: 99%