Flame Spectrophotometry of Calcium with Reversed Oxyacetylene Flame.  Application to Serum and Urine.

Löken, Hans F.; Teal, James S.; Eisenberg, Eugene

doi:10.1021/ac60200a033

Cited by 20 publications

(6 citation statements)

References 20 publications

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“…For these purposes we have used stable strontium intravenously, following the lead of Fraser et al (1960) but using the modifications and mathematical analysis of Eisenberg & Gordan (1961). Stable strontium is analysed flame spectrophotometrically at 461 m,u in a Beckman flame photometer with reversed acetylene and oxygen attachments to the sprayer burner; this is Mac-Intyre's (1957) method as modified by Loken, Teal & Eisenberg (1963). Serum and urine calcium and strontium levels are determined every twentyfour hours for four to six days.…”

mentioning

confidence: 99%

The Effect of Œstrogens, Androgens and Corticoids on Skeletal Kinetics in Man

Gordan

Eisenberg

1963

Proceedings of the Royal Society of Medicine

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confidence: 99%

The Effect of Œstrogens, Androgens and Corticoids on Skeletal Kinetics in Man

Gordan

Eisenberg

1963

Proceedings of the Royal Society of Medicine

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“…The levels of calcium in serum and urine were determined with a modified Beckman flame photometer by the method of Loken et al (1963). Phosphorus levels in serum and urine were determined by the method of Taussky Sc Shorr (1953).…”

Section: Methodsmentioning

confidence: 99%

Parathyroid Hormone and Phosphate Homeostasis in Man

Halden

Eisenberg

Gordan

1964

Acta Endocrinologica

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To determine whether parathyroid hormone is required for renal response to phosphorus loading, the effects of ingestion of 3100 mg of phosphorus daily for 3 days were compared in 5 treated hypoparathyroid patients and 5 normal control subjects of comparable age and sex. In both normal and hypoparathyroid subjects the response to phosphorus loading was characterized by an increase in urinary excretion of phosphorus without a concomitant increase in the rate of glomerular filtration of phosphorus. Some mechanism other than changes in rates of parathyroid hormone secretion probably accounts for renal responses to changes in phosphorus intake. The timing of urine and serum collections with regard to food intake and the total amount of phosphorus in the diet were found to affect the assessment of renal handling of phosphorus. The finding that a 3-day period of oral phosphorus loading did not increase the serum phosphorus level in the hypoparathyroid patients suggests that phosphorus restriction is not always necessary in the treatment of hypoparathyroidism. The addition of one more condition to the list of those that may affect the percentage of renal tubular resorption of phosphorus in no way decreases the usefulness of this determination in the diagnosis of hyperparathyroidism.

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“…Calcium was determined by the flame spectrophotometric method of Loken, Eisenberg and Teal ( 2 ) , based on that of MacIntyre(3) in which the calcium emission at 422.7 mp is measured, using an oxyacetylene flame, a Zeiss direct sprayer burner, a prism monochromator, and a IP2 1 photomultiplier tube, activated by 1200 volts. The method differs from that originally described by Mac-Intyre in that those ions present in urine and serum are added to the perchloric acid dilu-*Supported in part by grant from Nat.…”

Section: D~pnrtments Of Surgery Medicine and Nturosurgery Univtrsitmentioning

confidence: 99%

“…Comparison of infecti-ous canine hepatitis (ICH) virus with human adenoviruses indicates that ICH virus is a member of the adenovirus group ( 1,2,3). Dogs have been shown to be susceptible to human adenovirus inifection, and they develop complement-fixing and neutralizing antibodies !following a single inoculation (3,4).…”

Section: D~pnrtments Of Surgery Medicine and Nturosurgery Univtrsitmentioning

confidence: 99%

Urinary Calcium Excretion of Women with Breast Cancer during Post-Hypophysectomy Polyuria, Spontaneous Interphase and Vasopressin-Induced Antidiuresis.

Gardner

Gordan

Witt

1962

Experimental Biology and Medicine

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Thorn(1) has recently reported that small doses of porcine posterior pituitary extract increase urinary calcium excretion olf normal dogs. Availability of patients underguing hy-pophysectomy for objectively advancing disseminated breast cancer gave us the opportunity to measure urinary calcium excretion during spontaneous postoperative polyuria and after vasopressin administration, and thereby, to determine if a similar effect ca.n be demonstrated in human subjects.Methods. Eight patients with advanced breast cancer were studied before and after hypophysectomy. These patients had all received previous hormonal treatment with androgens, and subsequently, with a combination of prednisone, 30 mg/day and triiodothyronine, 50 pg/day, and were far along in the course of their disease. The patients received 200 mg of cortisone acetate daily, starting 24 hours before surgery. On the second postoperative day, the therapy was changed to hydrocortisone, 30 mg/day, which was maintained. Triiodothyronine, 50 pg/ day, was introduced after the fourteenth day. Twelve-hour nocturnal urinary collections were used to avoid the non-specific effects of varying degrees of daytime immobilization.Calcium was determined by the flame spectrophotometric method of Loken, Eisenberg and Teal ( 2 ) , based on that of MacIntyre(3) in which the calcium emission at 422.7 mp is measured, using an oxyacetylene flame, a Zeiss direct sprayer burner, a prism monochromator, and a IP2 1 photomultiplier tube, activated by 1200 volts. The method differs from that originally described by Mac-Intyre in that those ions present in urine and serum are added to the perchloric acid dilu-*Supported in part by grant from Nat. Inst. Health, and an institutional grant from Am. Cancer SOC. allocated by the Cancer Coordinating Committee, Univ. of California.ent in amounts adequate to produce a plateau augmentation of the calcium emission (recovery is 99.5-lW.S%).Creatinine was determined by the method of Folin and Wu (4). Phosphorus was measured by the method uf Taussky and Schorr (5). Tubular reabsorption of phosphate (TRY) was calculated by the method of Chambers, et al. (6) , using 12-hour urine mllections. No correction was made for protein binding of serum inorganic phosphate.Results and discussion. The results are summarized in Tables I-VIII. Evaluation of the urinary calcium and creatinine clearance during the first 3 postoperative days was complicated by the augmenting effect of large doses of cortisone( 7-9). Polyuria without interposition of an interphase developed in patient L.R. (Table I). A moderate, gradual decrease in urinary calcium excretion was noted in spite of the continued large urinary volume. Declines in calcium excretion and glomerular filtration following hypophysectomy have been noted previously( 10,ll) , although Pearson has recently stated that calcium and phosphorus balance are undisturbed by hypophysectomy ( 12 ) .The mean creatinine clearance in these hypophysectomized breast cancer patients was 56.8 -t 3.5 ml/min. (mean t S.E.) compared...

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Flame Spectrophotometry of Calcium with Reversed Oxyacetylene Flame. Application to Serum and Urine.

Cited by 20 publications

References 20 publications

The Effect of Œstrogens, Androgens and Corticoids on Skeletal Kinetics in Man

The Effect of Œstrogens, Androgens and Corticoids on Skeletal Kinetics in Man

Parathyroid Hormone and Phosphate Homeostasis in Man

Urinary Calcium Excretion of Women with Breast Cancer during Post-Hypophysectomy Polyuria, Spontaneous Interphase and Vasopressin-Induced Antidiuresis.

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