Flagellin as a good carrier and potent adjuvant for Th1 response: Study of mice immune response to the p27 (Rv2108) Mycobacterium tuberculosis antigen

P27-PPE36 (Rv2108) Mycobacterium tuberculosis Antigen – Member of PPE Protein Family with Surface Localization and Immunological Activities

“…DNA immunization was less potent in the induction of such responses. We confirmed the role of flagellin in this response by using different immunization combinations (Le Moigne et al, 2008). However, the specific antibody response was weak with either method (Fig.…”

“…The best cellular and humoral responses were obtained in the group of mice primed with the recombinant protein and boosted by the antigen presented on the modified flagellin (Le Moigne et al, 2008). In general, the P27-PPE36 PPE antigen induced a strong proliferative response accompanied by high production of IFN- and low amount of IL-4 (Le Moigne et al, 2008), independently of the PAMP used. The results indicated that this antigen may be involved in the establishment of the host cellular immune responses against the Mtb.…”

“…Different immunization protocols were used to study immunological potential of the P27-PPE36 protein. BALB/c mice were immunized either with the P27-PPE36 recombinant protein in Freund's adjuvant or in phosphate saline buffer (PBS) (classical immunization), with a pcDNA3 plasmid containing the gene encoding the P27-PPE36 protein (DNA immunization), or with the Escherichia coli bacteria expressing the P27-PPE36 protein genetically fused into the flagellin (flagellin immunization) (Le Moigne et al, 2008).…”

Antigen-Containing Liposomes Engrafted with Flagellin-Related Peptides Are Effective Vaccines That Can Induce Potent Antitumor Immunity and Immunotherapeutic Effect

“…Consistent with this, a number of studies showed that flagellin has adjuvant-like properties (17,18) Vaccination with recombinant flagellin-Ag fusions induced potent T and B cell responses, including Ag-specific CD8 + T cell and Ab responses to model Ags (19,20), as well as to a number of disease-relevant Ags (21)(22)(23), including influenza M2e Ag (24), Plasmodium vivax merozoite surface protein-1 (25), and Mycobacterium tuberculosis Ag p27 (26). The responses induced were often much greater than those induced by vaccination of the Ag emulsified in CFA (20)(21)(22)(23)(24)(25)(26). Also, the linking of flagellin to Ag is essential to induce detectable Ab responses, because a simple mixing of the two is not sufficient (20)(21)(22)(23)(24)(25)(26).…”

Chimeric flagellin expressed by Salmonella typhimurium induces an ESAT-6-specific Th1-type immune response and CTL effects following intranasal immunization

“…One study reported that the product of the gene Rv2108 of MTB expressed in flagellin also produced the strongest Th1-like immune response. 25 Manocha et al further demonstrated that intranasal immunization of mice with Fla-91-a recombinant flagellin containing the influenza B-cell epitope HA91-108-led to partial protection of mice from viral challenge. 26 High-level T-cell responses and increased IL-2 or IFN-c cytokine production from PPs have also been detected following i.n.…”