Flagellin: a unique microbe-associated molecular pattern and a multi-faceted immunomodulator

Zhong, Ma; Yan, Huimin; Li, Yaoming

doi:10.1038/cmi.2017.78

Cited by 16 publications

(12 citation statements)

References 14 publications

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“…We once developed a kind of recombinant flagellin KFD as a mucosal adjuvant, which was proven to be safe and effective for intranasal immunization in our previous works [ 11 , 12 ]. KFD exerts its immune modulating activity by activating the TLR5 pathway in nasal epithelial cells and enhancing local and distal mucosal IgA responses [ 13 , 14 ]. In this study, the immunogenicity of 3Ro-NC was assessed by intramuscular injection with an aluminum adjuvant (AL-adjuvant) or by intranasal vaccination with a recombinant flagellin protein KFD adjuvant (KFD).…”

Section: Introductionmentioning

confidence: 99%

A triple-RBD-based mucosal vaccine provides broad protection against SARS-CoV-2 variants of concern

Yang

Liu

et al. 2022

Cell Mol Immunol

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The rapid mutation and spread of SARS-CoV-2 variants urge the development of effective mucosal vaccines to provide broad-spectrum protection against the initial infection and thereby curb the transmission potential. Here, we designed a chimeric triple-RBD immunogen, 3Ro-NC, harboring one Delta RBD and two Omicron RBDs within a novel protein scaffold. 3Ro-NC elicits potent and broad RBD-specific neutralizing immunity against SARS-CoV-2 variants of concern. Notably, intranasal immunization with 3Ro-NC plus the mucosal adjuvant KFD (3Ro-NC + KFDi.n) elicits coordinated mucosal IgA and higher neutralizing antibody specificity (closer antigenic distance) against the Omicron variant. In Omicron-challenged human ACE2 transgenic mice, 3Ro-NC + KFDi.n immunization significantly reduces the tissue pathology in the lung and lowers the viral RNA copy numbers in both the lung (85.7-fold) and the nasal turbinate (13.6-fold). Nasal virologic control is highly correlated with RBD-specific secretory IgA antibodies. Our data show that 3Ro-NC plus KFD is a promising mucosal vaccine candidate for protection against SARS-CoV-2 Omicron infection, pathology and transmission potential.

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Section: Introductionmentioning

confidence: 99%

A triple-RBD-based mucosal vaccine provides broad protection against SARS-CoV-2 variants of concern

Yang

Liu

et al. 2022

Cell Mol Immunol

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“…Considerable evidence indicates that FliC functions as a PAMP in the control of pathogenic infections caused by bacteria by binding to the surface or cytosolic receptor of host cells ( 41 , 42 , 43 , 44 ). To identify the candidate interactive protein of flagellin, we used flagellum monomers of FliC from V. splendidus AJ01 as the target to isolate the potential interacting protein from sea cucumber coelomocytes.…”

Section: Resultsmentioning

confidence: 99%

Vibrio splendidus flagellin C binds tropomodulin to induce p38 MAPK-mediated p53-dependent coelomocyte apoptosis in Echinodermata

Dai

Guo

Shao

et al. 2022

Journal of Biological Chemistry

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“…In this process, the pattern recognition receptors recognize the conserved molecular patterns like lipopolysaccharides, peptidoglycans, chitin, flagellin, EF-Tu, DNA and ergosterol known as pathogen-associated molecular patterns (PAMPs) or microbe-associated molecular patterns (MAMPs), which aids in the hydrolysis and activation of signaling pathways including production of reactive oxygen species (ROS), MAP kinase activation and transcriptional induction of pathogen-responsive genes. Several excellent reviews of MAMPs are available [7][8][9][10]. The microbial and pathogen-associated molecular patterns are slow processes as they evolve over a considerable period [6].…”

Section: Pattern-triggered Immunity (Pti)mentioning

confidence: 99%

Signaling Pathways and Downstream Effectors of Host Innate Immunity in Plants

Kumar

Ramlal

Kumar

et al. 2021

IJMS

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Phytopathogens, such as biotrophs, hemibiotrophs and necrotrophs, pose serious stress on the development of their host plants, compromising their yields. Plants are in constant interaction with such phytopathogens and hence are vulnerable to their attack. In order to counter these attacks, plants need to develop immunity against them. Consequently, plants have developed strategies of recognizing and countering pathogenesis through pattern-triggered immunity (PTI) and effector-triggered immunity (ETI). Pathogen perception and surveillance is mediated through receptor proteins that trigger signal transduction, initiated in the cytoplasm or at the plasma membrane (PM) surfaces. Plant hosts possess microbe-associated molecular patterns (P/MAMPs), which trigger a complex set of mechanisms through the pattern recognition receptors (PRRs) and resistance (R) genes. These interactions lead to the stimulation of cytoplasmic kinases by many phosphorylating proteins that may also be transcription factors. Furthermore, phytohormones, such as salicylic acid, jasmonic acid and ethylene, are also effective in triggering defense responses. Closure of stomata, limiting the transfer of nutrients through apoplast and symplastic movements, production of antimicrobial compounds, programmed cell death (PCD) are some of the primary defense-related mechanisms. The current article highlights the molecular processes involved in plant innate immunity (PII) and discusses the most recent and plausible scientific interventions that could be useful in augmenting PII.

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Flagellin: a unique microbe-associated molecular pattern and a multi-faceted immunomodulator

Cited by 16 publications

References 14 publications

A triple-RBD-based mucosal vaccine provides broad protection against SARS-CoV-2 variants of concern

A triple-RBD-based mucosal vaccine provides broad protection against SARS-CoV-2 variants of concern

Vibrio splendidus flagellin C binds tropomodulin to induce p38 MAPK-mediated p53-dependent coelomocyte apoptosis in Echinodermata

Signaling Pathways and Downstream Effectors of Host Innate Immunity in Plants

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