Flagellar motility contributes to the invasion and survival of Aeromonas hydrophila in Anguilla japonica macrophages

Qin, Yingxue; Lin, Guifang; Chen, Wenbo; Huang, Bei; Huang, Wei‐Jan; Yan, Qingpi

doi:10.1016/j.fsi.2014.05.016

Cited by 42 publications

(46 citation statements)

References 33 publications

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“…Disruption of the Hr gene in A. hydrophila mutants (M85) led to a 77 % reduction in survival when incubated with macrophages. Virulence of A. hydrophila is dependent on its ability to escape phagosomes using flagellar movements [201]. No differences in motility were found between the wild-type and Hr mutants; therefore, Hr is an important factor in A. hydrophila pathogenesis.…”

Section: Hemerythrin and Innate Immunitymentioning

confidence: 99%

Immunological properties of oxygen-transport proteins: hemoglobin, hemocyanin and hemerythrin

Coates

Decker

2016

Cell. Mol. Life Sci.

119

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It is now well documented that peptides with enhanced or alternative functionality (termed cryptides) can be liberated from larger, and sometimes inactive, proteins. A primary example of this phenomenon is the oxygen-transport protein hemoglobin. Aside from respiration, hemoglobin and hemoglobin-derived peptides have been associated with immune modulation, hematopoiesis, signal transduction and microbicidal activities in metazoans. Likewise, the functional equivalents to hemoglobin in invertebrates, namely hemocyanin and hemerythrin, act as potent immune effectors under certain physiological conditions. The purpose of this review is to evaluate the true extent of oxygen-transport protein dynamics in innate immunity, and to impress upon the reader the multi-functionality of these ancient proteins on the basis of their structures. In this context, erythrocyte–pathogen antibiosis and the immune competences of various erythroid cells are compared across diverse taxa.Electronic supplementary materialThe online version of this article (doi:10.1007/s00018-016-2326-7) contains supplementary material, which is available to authorized users.

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Section: Hemerythrin and Innate Immunitymentioning

confidence: 99%

Immunological properties of oxygen-transport proteins: hemoglobin, hemocyanin and hemerythrin

Coates

Decker

2016

Cell. Mol. Life Sci.

119

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“…Interestingly, it has been reported that motility is required for A. hydrophila to invade and survive in macrophages, as it contributes to the escape from phagosomes or lysosomes into the cytoplasm (Qin et al, 2014). Sano et al (2007) also reported that Salmonella enterica use flagella to escape from host macrophages.…”

Section: Discussionmentioning

confidence: 99%

“…This bacterium is not only widely considered a major fish and amphibian pathogen, but also causes gastroenteritis, cellulitis, and other diseases in humans (Liu, 2009;Chao et al, 2013). Our previous studies revealed that A. hydrophila can invade and survive in host Anguilla japonica macrophages in vitro (Qin et al, 2014). However, knowledge on the mechanisms of A. hydrophila invasion and survival in these host macrophages remains limited.…”

Section: Introductionmentioning

confidence: 99%

Hemerythrin is required for Aeromonas hydraphlia to survive in the macrophages of Anguilla japonica

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Survival in host phagocytes is an effective strategy for pathogenic microbes to spread. To understand the mechanisms of Aeromonas hydrophila survival within host macrophages, a library of mini-Tn10 transposon insertion mutants was constructed. The M85 mutant, whose survival in host macrophages was only 23.1% of that of the wild-type (WT) strain, was utilized for further study. Molecular analysis showed that a 756-bp open reading frame (ORF) (GenBank accession No. CP007576) in the M85 mutant was interrupted by miniTn10. This ORF encodes for a 183-amino acid protein and displays the highest sequence identity (99%) with the hemerythrin (Hr) protein of A. hydrophila subspecies hydrophila ATCC 7966. The survival of the WT, M85 mutant, and complemented M85 (Hr) strains were compared in host macrophages in vitro, and the results showed that M85 exhibited defective survival, while that of M85 (Hr) was restored. To investigate the possible mechanisms of A. hydrophila survival in host macrophages, the expression of Hr under hyperoxic and hypoxic conditions was evaluated. The results revealed that the expression of this protein was higher under hyperoxic conditions than under hypoxic conditions, which indicates that Hr protein expression is sensitive to O 2 concentration. Hydrogen peroxide sensitivity tests further suggested that the M85 mutant was more sensitive to oxidative stress than the WT and M85 (Hr) strains. Taken together, these results suggest that the Hr protein may act as an O 2 sensor and as a detoxifier of reactive oxygen species, and is required for A. hydrophila survival within host macrophages.

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“…27 In addition, a flagellum is required for A. hydrophila survival in the macrophages of its host. 28 However, although numerous studies have identified factors reportedly associated with A. hydrophila virulence, further studies of the mechanisms by which it evades the immune system are still necessary.…”

Section: Discussionmentioning

confidence: 99%

Contribution of nuclease to the pathogenesis of Aeromonas hydrophila

Qin

et al. 2015

Virulence

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These authors contributed equally to this work. Keywords: Aeromonas hydrophila, nuclease, pathogenesisAeromonas hydrophila is a gram-negative bacterium that is widely distributed in aquatic environments and can cause septicemia in both fish and humans. However, the underlying mechanisms leading to severe infection are not well understood. In this study, an A. hydrophila nuclease (ahn) deletion mutant was constructed to investigate its contribution to pathogenesis. This mutant did not differ from the wild-type strain in terms of its growth or hemolytic phenotype. However, the ahn-deficient mutant was more susceptible to being killed by fish macrophages and mouse blood in vitro. Furthermore, evidence obtained using both fish and murine infection models strongly indicated that the inactivation of Ahn impaired the ability of A. hydrophila to evade innate immune clearance in vivo. More importantly, the virulence of the mutant was attenuated in both fish and mice, with reductions in dissemination capacities and mortality rates. These findings implicate Ahn in A. hydrophila virulence, with important functions in evading innate immune defenses.

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Flagellar motility contributes to the invasion and survival of Aeromonas hydrophila in Anguilla japonica macrophages

Cited by 42 publications

References 33 publications

Immunological properties of oxygen-transport proteins: hemoglobin, hemocyanin and hemerythrin

Immunological properties of oxygen-transport proteins: hemoglobin, hemocyanin and hemerythrin

Hemerythrin is required for Aeromonas hydraphlia to survive in the macrophages of Anguilla japonica

Contribution of nuclease to the pathogenesis of Aeromonas hydrophila

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