Flagellar length depends on LdARL-3A GTP/GDP unaltered cycling in Leishmania amazonensis

Sahin, Annelise; Espiau, Benoît; Marchand, Cécile; Merlin, Gilles

doi:10.1016/j.molbiopara.2007.08.003

Cited by 9 publications

(11 citation statements)

References 23 publications

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“…A role for the ADP-ribosylation factor-like protein, Ld ARL-3A, in the regulation of flagellar length in Leishmania comes from studies utilizing overexpression of enzymatically inactive forms of Ld ARL-3A [53, 54]. ARLs are small G proteins that are members of the ras superfamily and as such transit between a GDP and GTP bound form.…”

Section: Genes Involved In the Regulation Of Flagellar Lengthmentioning

confidence: 99%

Regulation of flagellar length in Chlamydomonas

Wilson

Iyer

Buchheim

et al. 2008

Seminars in Cell & Developmental Biology

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Chlamydomonas reinhardtii has two apically localized flagella that are maintained at an equal and appropriate length. Assembly and maintenance of flagella requires a microtubule-based transport system known as intraflagellar transport (IFT). During IFT, proteins destined for incorporation into or removal from a flagellum are carried along doublet microtubules via IFT particles. Regulation of IFT activity therefore is pivotal in determining the length of a flagellum. Reviewed is our current understanding of the role of IFT and signal transduction pathways in the regulation of flagellar length.

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Section: Genes Involved In the Regulation Of Flagellar Lengthmentioning

confidence: 99%

Regulation of flagellar length in Chlamydomonas

Wilson

Iyer

Buchheim

et al. 2008

Seminars in Cell & Developmental Biology

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“…A number of proteins including ADP-ribosylation factor proteins (ARL, also known as small G-proteins [76][77][78]) play a pivotal role in intraflagellar protein trafficking, flagellum integrity and assembly. We have identified isoforms of two ARL proteins (LinJ.29.0950 and LinJ.30.2380 in Table S3) that seem to be uniquely expressed in the metacyclic forms of both parasites.…”

Section: Motility and Morphology-related Proteinsmentioning

confidence: 99%

Proteomic analysis of metacyclogenesis in Leishmania infantum wild-type and PTR1 null mutant

Moreira

Légaré

Racine

et al. 2014

EuPA Open Proteomics

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Reduced pterinsFolates Metacyclogenesis Free-flow electrophoresis a b s t r a c tThe parasite Leishmania exhibits a dimorphic life cycle with promastigotes found in the insect vector and amastigotes residing within mammalian macrophages. In the insect, promastigotes undergo metacyclogenesis with two main stages, the procyclic and metacyclic stages. Reduced pterins, provided by the pteridine reductase PTR1, are important factors controlling metacyclogenesis. We applied here the liquid-based free-flow electrophoresis technique for the analysis of L. infantum wild-type and PTR1 null-mutant procyclic and metacyclic proteomes. Significant modulations in energy metabolism, antioxidant and stress-related defences, genetic information processing, protein degradation and motility were observed between procyclic and metacyclic forms, in addition to numerous hypothetical proteins.

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“…Although SMP-1 is one of the first flagellar membrane proteins shown to have a direct role in flagellum biogenesis, an increasing number of non-flagellar proteins are thought to regulate protein and lipid trafficking to the flagellar membrane (Avidor-Reiss et al, 2004; Cuvillier et al, 2000;Dwyer et al, 2001;Hu et al, 2007;Nachury et al, 2007;Pullen et al, 2004;Qin et al, 2005;Rosenbaum and Witman, 2002;Sahin et al, 2008a;Scholey and Anderson, 2006;Wei et al, 2008). Several of these proteins have defined roles in regulating membrane trafficking between the Golgi complex, endosomes and the plasma membrane.…”

Section: Journal Of Cell Science 123 (4)mentioning

confidence: 99%

“…These mutants accumulate sterols in endocytic compartments and are hypersensitive to both sterol and sphingolipid biosynthetic inhibitors indicating a general perturbation of lipid homeostasis (Vince et al, 2008). Similarly, overexpression of a GTP-fixed form of the small myristoylated Arl3A protein in L. amazonensis induces defects in post-Golgi endosome trafficking and a dramatic shortening of the flagellum (Sahin et al, 2008a;Sahin et al, 2008b). Genetic deletion of a number of other Leishmania cytoplasmic proteins also results in retraction of the flagellum (Tammana et al, 2008;Thiel et al, 2008;Wiese, 2007), although the precise role of these proteins in membrane transport or signaling is unknown.…”

Section: Journal Of Cell Science 123 (4)mentioning

confidence: 99%

“…Unlike axonemal and matrix proteins that are synthesized in the cytosol and transported to flagella by intraflagellar transport (IFT) protein complexes, flagellar membrane proteins are synthesized in the ER and transported to the plasma and flagellar membrane via the Golgi complex. Vesicle transport to the flagellum appears to be regulated by a number of specific effector proteins (Rab8, Arl-3), cargo adaptors (AP-1 subunit, STAM) (Bae et al, 2006;Dwyer et al, 2001;Hu et al, 2007), phosphoinositides (Wei et al, 2008) and non-IFT cytoplasmic protein complexes (BardetBiedi syndrome, OSEG and exocyst proteins) (Avidor-Reiss et al, 2004;Cuvillier et al, 2000;Dwyer et al, 2001;Hu et al, 2007;Nachury et al, 2007;Pullen et al, 2004;Qin et al, 2005;Rosenbaum and Witman, 2002;Sahin et al, 2008a;Scholey and Anderson, 2006;Wei et al, 2008;Zuo et al, 2009). Although it is generally assumed that the axoneme provides the structural template for the flagellar membrane, the extent to which flagellar membrane dynamics regulate axoneme biogenesis and maintenance is not understood.…”

Section: Introductionmentioning

confidence: 99%

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Membrane protein SMP-1 is required for normal flagellum function inLeishmania

Tull

Naderer

Spurck

et al. 2010

Journal of Cell Science

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SummaryEukaryotic flagella and cilia are surrounded by a membrane that is continuous with, but distinct from, the rest of the plasma membrane. In Leishmania parasites, the inner leaflet of the flagellar membrane is coated with the acylated membrane protein, SMP-1. Here, we provide evidence that SMP-1 stabilizes the flagellar membrane and is required for flagella elongation and function. The expression and flagella targeting of SMP-1 is tightly associated with flagella elongation during amastigote to promastigote differentiation. Deletion of the genes encoding SMP-1 and the flagellar pocket protein SMP-2, led to the production of short flagella and defects in motility. Alterations in the physical properties of the smp-1/smp-2 -/-flagellar membrane were suggested by: (1) the accumulation of membrane vesicles in the flagellar matrix, and (2) further retraction of flagella following partial inhibition of sterol and sphingolipid biosynthesis. The flagella phenotype of the smp-1/smp-2 -/-null mutant was reversed by re-expression of SMP-1, but not SMP-2. SMP-1 contains a jelly-roll -sheet structure that is probably conserved in all SMP proteins, and forms stable homo-oligomers in vivo. We propose that the SMP-1 coat generates and/or stabilizes sterol-and sphingolipid-rich domains in the flagellar membrane.

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Flagellar length depends on LdARL-3A GTP/GDP unaltered cycling in Leishmania amazonensis

Cited by 9 publications

References 23 publications

Regulation of flagellar length in Chlamydomonas

Regulation of flagellar length in Chlamydomonas

Proteomic analysis of metacyclogenesis in Leishmania infantum wild-type and PTR1 null mutant

Membrane protein SMP-1 is required for normal flagellum function inLeishmania

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