Flagellar gene fliP contributes to the virulence of Pseudomonas plecoglossicida by regulating its motility

He, Li; Zhao, Lingmin; Li, Qi; Huang, Lixing; Qin, Yingxue; Zhuang, Zhixia; Wang, Xiaoru; Huang, Huabin; Zhang, Jiaonan; Zhang, Jiaolin; Yan, Qingpi

doi:10.1016/j.aquaculture.2023.739874

Cited by 7 publications

(2 citation statements)

References 59 publications

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“…Bacteria use agella for mobility in adverse environments and movement to less hostile sites suitable for adaptation and survival. They are usually formed by multiple protein subunits (He et al 2023). Flagella assembly is highly ordered and governed by a hierarchical mode of regulation that is tightly controlled by at least 17 operons composed of more than 50 genes (Chevance and Hughes, 2008).…”

Section: Unique Genesmentioning

confidence: 99%

Comparative genomic analysis and genome sequence of Halomonas salifodinae strain A2, isolated from the Zapotitlán Salinas Valley Puebla, México

León-Lemus,

-García,

Cabirol

et al. 2024

Preprint

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Here, we report the genome sequence of strain A2. The genome size was 3,855,926 bp, the GC content was 67.4%, and it contains 3509 genes, 62 tRNA, eight rRNA, and four snRNA. Phylogenetic analysis of the 16 S rRNA gene in the RDP, NCBI, and TYGS databases indicates that strain A2 belongs to Halomonas salifodinae. Also, MLSA analysis confirms that A2 is closely related to H. salifodinae. Phylogenomic and comparative genomic analysis using the ANIs and dDDH indicators classify H. salifodinae A2 and Bisbaumannia pacifica NBRC 102220 in a separate phylogenetic group of the genus Halomonas. The phylogenomic and pangenome analysis support the above, placing H. salifodinae A2 in a separate group with B. pacifica NBRC 102220. The pangenomic analysis shows 136,122 genes that comprise the pangenome with 317 core genes, 3457 shell genes, 132,332 accessory genome, and 691 unique genes. We found 29 genes for secretion systems in the genome analysis, 23 for Na + and K + ion transport, 6 BGC groups, a total of 12 genomic islands, an 8.2Kb gene prophage region, 15 regions associated with CRISPR and one CAS-TypeIF cas gene cluster region, 12 genes of biotechnological importance, 38 unique genes essential for adaptability and biotechnological relevance, as well as, 35 genes for the synthesis of compatible solutes. Furthermore, we propose the reclassification of the species within the genus Bisbaumannia.

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Section: Unique Genesmentioning

confidence: 99%

Comparative genomic analysis and genome sequence of Halomonas salifodinae strain A2, isolated from the Zapotitlán Salinas Valley Puebla, México

León-Lemus,

-García,

Cabirol

et al. 2024

Preprint

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“…Bacterial diseases, induced by various factors, continue to represent a considerable challenge within the aquaculture industry ( 1 – 4 ). Pseudomonas plecoglossicida , a conditionally pathogenic bacterium with a polar flagellum, causes a wide range of fish diseases, predominantly under low-temperature conditions ( 5 – 8 ). Recent reports have underscored the high infectivity and mortality rates of visceral white spot disease in large yellow croakers ( Larimichthys crocea ) and orange-spotted groupers ( Epinephelus coioides ) ( 6 , 9 ).…”

Section: Introductionmentioning

confidence: 99%

Role of the Pseudomonas plecoglossicida fliL gene in immune response of infected hybrid groupers (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂)

Shi,

Zhao,

et al. 2024

Front. Immunol.

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Pseudomonas plecoglossicida, a gram-negative bacterium, is the main pathogen of visceral white-point disease in marine fish, responsible for substantial economic losses in the aquaculture industry. The FliL protein, involved in torque production of the bacterial flagella motor, is essential for the pathogenicity of a variety of bacteria. In the current study, the fliL gene deletion strain (ΔfliL), fliL gene complement strain (C-ΔfliL), and wild-type strain (NZBD9) were compared to explore the influence of the fliL gene on P. plecoglossicida pathogenicity and its role in host immune response. Results showed that fliL gene deletion increased the survival rate (50%) and reduced white spot disease progression in the hybrid groupers. Moreover, compared to the NZBD9 strain, the ΔfliL strain was consistently associated with lower bacterial loads in the grouper spleen, head kidney, liver, and intestine, coupled with reduced tissue damage. Transcriptomic analysis identified 2 238 differentially expressed genes (DEGs) in the spleens of fish infected with the ΔfliL strain compared to the NZBD9 strain. Based on Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, the DEGs were significantly enriched in seven immune system-associated pathways and three signaling molecule and interaction pathways. Upon infection with the ΔfliL strain, the toll-like receptor (TLR) signaling pathway was activated in the hybrid groupers, leading to the activation of transcription factors (NF-κB and AP1) and cytokines. The expression levels of proinflammatory cytokine-related genes IL-1β, IL-12B, and IL-6 and chemokine-related genes CXCL9, CXCL10, and CCL4 were significantly up-regulated. In conclusion, the fliL gene markedly influenced the pathogenicity of P. plecoglossicida infection in the hybrid groupers. Notably, deletion of fliL gene in P. plecoglossicida induced a robust immune response in the groupers, promoting defense against and elimination of pathogens via an inflammatory response involving multiple cytokines.

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Identification and characteristics of temperature-sensitive sites of Pseudomonas plecoglossicida based on aptamers

Fan,

Xu,

Lin

et al. 2023

Aquaculture Reports

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Flagellar gene fliP contributes to the virulence of Pseudomonas plecoglossicida by regulating its motility

Cited by 7 publications

References 59 publications

Comparative genomic analysis and genome sequence of Halomonas salifodinae strain A2, isolated from the Zapotitlán Salinas Valley Puebla, México

Comparative genomic analysis and genome sequence of Halomonas salifodinae strain A2, isolated from the Zapotitlán Salinas Valley Puebla, México

Role of the Pseudomonas plecoglossicida fliL gene in immune response of infected hybrid groupers (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂)

Identification and characteristics of temperature-sensitive sites of Pseudomonas plecoglossicida based on aptamers

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