Fixed-time insemination with frozen semen in mares: is it suitable for poorly fertile stallions?

Avanzi, Bruno Ribeiro; Ramos, Renata dos Santos; Araújo, G. H. M.; Fioratti, Eduardo Gorzoni; Trinca, Luzia A.; Dell’Aqua, J.A.; Oña, Cely Marini Melo e; Zahn, Fabíola Soares; Martin, Ian; Alvarenga, Marco Antônio; Papa, Frederico Ozanam

doi:10.1016/j.theriogenology.2014.07.007

Cited by 13 publications

(16 citation statements)

References 27 publications

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“…Data on fertility with the use of equine frozen semen in BotuCrio extender containing LDL were not found. Despite the low number of inseminated mares, the fertility rate obtained was close to that found by other researchers who used only one insemination postovulation and semen frozen in extenders containing cryoprotectants from amides group (Oliveira et al, 2013;Avanzi et al, 2015). Fertility rates between 40 and 82.3% were reported after the use of two inseminations using an extender containing 4% of methylformamide and 1% glycerol (Melo et al, 2007).…”

Section: Discussionsupporting

confidence: 82%

Can we use LDL instead of egg yolk in BotuCrio® extender to cryopreserve sperm from the Mangalarga Marchador stallion?

Snoeck¹,

Pessoa²,

Pereira³

et al. 2019

Anim Reprod

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The objective of this study was to compare the BotuCrio ® extender with the Merk-egg yolk and the INRA 82 modified by the inclusion of acetamide, methyl cellulose and trehalose in substitution of glycerol for freezing equine semen. The semen was diluted after centrifugation to obtain 100 x 10 6 of sperm/ml in: BotuCrio ® (control); Merk-egg yolk or INRA 82 modified (Experiment 1). The extended semen was packaged in 0.5 ml straws, cooled and frozen in a freezing machine. The control extender was superior in preserving the motility, VCL, VSL, VAP, LIN, STR and the BCF when compared to the Merk-egg yolk and INRA 82 modified (P < 0.05). The BotuCrio® preserved more effectively the equine sperm viability characteristics evaluated in Experiment 1 and was used as a control extender in Experiment 2 to test the effectiveness of using LDL in replacement of egg yolk. BotuCrio ® was superior to preserve progressive motility, VCL, VSL, VAP, LIN, STR and the percentage of functional integrity of sperm membranes compared to BotuCrio LDL (P < 0.05). However, both extenders preserved similarly the total motility, ALH, BCF and the structural integrity of the membranes (P > 0.05). The fertility rate after AI with frozen semen in BotuCrio LDL was 37.5%.

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Section: Discussionsupporting

confidence: 82%

Can we use LDL instead of egg yolk in BotuCrio® extender to cryopreserve sperm from the Mangalarga Marchador stallion?

Snoeck¹,

Pessoa²,

Pereira³

et al. 2019

Anim Reprod

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“…The studies on the basis of the cryopreservation of stallion sperm have been reported for more than 50 years [15][16][17][18][19], however, the researches involving donkey semen are recent [20][21][22][23][24]. Despite the excellent semen quality, fertility rates of frozen donkey semen in jennies are still low (0%-36%) [20][21][22] compared to mares (33%-53%) [25,26].…”

Section: Introductionmentioning

confidence: 99%

Strategies to improve the fertility of fresh and frozen donkey semen

Oliveira

Oña

et al. 2016

Theriogenology

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Fertility rates of donkey semen in jennies are lower compared to mares. The aims of this study were to evaluate different sperm cryopreservation methods and insemination strategies to improve the fertility of donkey semen in jennies. Three experiments were performed: (1) the comparison of two freezing methods of donkey semen (conventional method and automated method); (2) the determination of a suitable insemination dose of fresh donkey semen for jennies and mares; and (3) the influence of the semen deposition site on fertility of jennies inseminated with frozen donkey semen. For experiment 1, no differences were observed in total motility, angular velocity, curvilinear velocity, straight-line velocity, and plasma membrane integrity between samples frozen with the conventional (Styrofoam box) and the automated method (TK 4000C). However, the automated method provided higher values of progressive motility and rapid cells in frozen-thawed samples in comparison with the conventional method (P < 0.05). For experiment 2, mares were bred using 500 × 10(6) fresh sperm (M); and jennies using 1 × 10(9) (J1) or 500 × 10(6) fresh sperm (J5). Pregnancy rates in M, J1, and J5 were 93% (14/15), 73% (11/15), and 40% (6/15), respectively. When using different insemination doses, 500 × 10(6) or 1 × 10(9) sperm, no significant difference was observed in pregnancy rates of mares (M, 14/15) and jennies (J1, 11/15). Furthermore, there was no significant difference between the two insemination doses in jennies. However, with an insemination dose of 500 × 10(6) fresh sperm, the pregnancy rates were significantly higher in mares (M, 14/15) than in jennies (J5, 6/15; P < 0.05). For experiment 3, the inseminations were carried out in the uterine body (UB) or in the uterine horn of jennies with frozen-thawed donkey semen. No pregnancies were achieved with inseminations performed in the UB (0/12). The pregnancy rate for uterine horn group was 28.26% (13/46) and thus significantly higher than the UB group (0%; 0/12; P < 0.05). In conclusion, the automated method showed higher values on progressive motility and rapid cells parameters compared to the conventional method and can be used as an alternative for freezing donkey semen. The increase in the number of sperm cells per insemination dose using fresh donkey semen improved the fertility rates in jennies. The deep horn inseminations using frozen-thawed donkey semen increased the pregnancy rate in jennies, and the multiple inseminations may be an option to improve the fertility rates of donkey semen in jennies.

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“…POFs have been extensively used in breeding protocols. Employing POFs in biotechniques such as artificial insemination [AVANZI et al, 2015], embryo transfer [PESSOA et al, 2011], intracytoplasmic injection of spermatozoa [SESSIONS-BRESNAHAN et al, 2014], and use of sexed semen [SAMPER et al, 2012] has led to considerable advancements in the areas of reproductive physiology, gametogenesis, and embryogenesis [AURICH, 2012].…”

Section: Introductionmentioning

confidence: 99%

Follicle Populations in Mare Ovaries: From Preantral to Preovulatory Follicles

Leonel¹,

Bento-Silva²,

Silva³

et al. 2015

Enciclopédia Biosfera

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The purpose of this study was to describe morphological and quantitative aspects of preantral and antral follicles in the equine ovary. Six ovaries were collected from necropsied mares and sliced lengthwise parallel to the ovulation fossa. The slices were fixed and processed for histological evaluation under light microscopy. Preantral follicles were classified according to developmental stage and as morphologically normal or degenerated. Also, another 183 ovaries were obtained from an abattoir. From these ovaries, antral follicles were measured, punctured and oocytes evaluated. Oocyte recovery rates were calculated for each follicle size category. A total of 490 preantral follicles were examined. Primary follicles were in greater number than primordial follicles. No secondary follicles were identified. A total of 518 antral follicles were punctured and evaluated. Total oocyte recovery rate from antral follicles was 47.6. In conclusion, preantral follicles consist of a scarce source in the equine ovary. Antral follicles oocyte recovery rate was higher for smaller-diameter antral follicles.

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Fixed-time insemination with frozen semen in mares: is it suitable for poorly fertile stallions?

Cited by 13 publications

References 27 publications

Can we use LDL instead of egg yolk in BotuCrio® extender to cryopreserve sperm from the Mangalarga Marchador stallion?

Can we use LDL instead of egg yolk in BotuCrio® extender to cryopreserve sperm from the Mangalarga Marchador stallion?

Strategies to improve the fertility of fresh and frozen donkey semen

Follicle Populations in Mare Ovaries: From Preantral to Preovulatory Follicles

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