Fixation of phycobiliproteins to photosynthetic membranes by glutaraldehyde

Cohen-Bazire, Germaine; Lefort‐Tran, M.

doi:10.1007/bf00410158

Cited by 30 publications

(14 citation statements)

References 19 publications

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“…11). However, Cohen-Bazire and Lefort-Tran [36] have reported a depression in the concentration of phycoerythrin although no change in energy transfer from phycocyanin to Chl a was observed upon fixation [37]. The DCPIP reduction in the fixed cells, suspended in a phosphate buffer at p H 6.8, was found to be about 30-80 pmoles of dye reduced/mg-Chl/hr in saturating white light.…”

Section: Fixation Of Photosynthetic Membranes By Glutaraldehyde Andjumentioning

confidence: 90%

FLUORESCENCE INDUCTION IN THE RED ALGA PORPHYRIDIUM CRUENTUM

Mohanty

Papageorgiou

1971

Photochem & Photobiology

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The intensity dependence and the spectral changes during the fast (sec) and the slow (min) transient of chlorophyll (Chl) a fluorescence yield, measured at 685nm, have been analyzed in the red alga Porphyridium cruentum. Both the fast and the slow fluorescence yield changes are affected differently by the inhibitors of electron transport (e.g., DCMU) and by the uncouplers of phosphorylation (atebrin and FCCP). Fixation of Porphyridium cells with glutaraldehyde abolishes most of the fluorescence yield changes except for the so-called very fast (01) phase. The same fixed cells, however, reduce DCPIP (a Hill oxidant) but do not evolve 0, when CO, is used as electron acceptor. We interpret these and other results by the hypothesis that fluorescence transients in intact cells are linked to both electron transport and the energy dependent structural changes in the thylakoid membrane.

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Section: Fixation Of Photosynthetic Membranes By Glutaraldehyde Andjumentioning

confidence: 90%

FLUORESCENCE INDUCTION IN THE RED ALGA PORPHYRIDIUM CRUENTUM

Mohanty

Papageorgiou

1971

Photochem & Photobiology

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“…Light micrographs of eral months on the chloroplast fraction, band 1, and the blue-green algal frac-1 leaflets have tion, band 3, are shown in Figure 5, (10,19). In addition, the strong fluorescence emission from phycocyanin at 640 nm, observed when the algal fraction was illuminated with 560 nm light at 77 K, was not detected in the chloroplast fraction.…”

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confidence: 98%

“…4 C (22, 26), Three of the sequential treatments were successful and gave ide CA350 (6 fronds which have remained free of the symbiont and incapapproaches was ble of acetylene reduction. These were: (a) Aureomycin 2 ventually gave ,ug/ml to Na penicillin G 50 ,ug/ml to streptomycin sulfate 10 dividually and ytg/ml to bacitracin 10 jug/ml and polymyxon B sulfate 12.5 each instance, jug/ml; (b) polymyxin sulfate 25 ,ug/rnl to tetracycline 10 combined N2 jug/ml to bacitracin 20 jug/ml to streptomycin sulfate 20 jug/ml trient medium and Na penicillin G 100 ,ug/ml; (c) tetracycline 10 ,tg/ml, *esh antibiotic-polymyxin B sulfate 25 jug/ml, bacitracin 20 ,ug/ml to Na penicillin G 100 ,ug/ml, streptomycin sulfate 20 /tg/ml, Aureomycin 4 jug/ml to tetracycline-HCl 10 ,tg/ml, polymyxin B sulfate 25 ,ug/ml, bacitracin 20 ,ug/ml to Na penicillin G 100 yg/ml, streptomycin sulfate 20 ,ug/ml, Aureomycin 4 [ktg/ml.…”

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confidence: 99%

The Azolla, Anabaena azollae Relationship

Peters

Mayne²

1974

Plant Physiol.

160

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The Azolla-Anabaena azollae association permits the study of a symbiotic relationship between a blue-green alga and a green plant under laboratory conditions. Previous studies on the physiology of the symbiotic association were not well defined and were limited in scope. Various aspects of mineral nutrition, temperature, and light intensity on the growth of the organism have been reported (22). We are not aware of any studies on the metabolic functions of N2 fixation, respiration, and photosynthesis in the individual organisms or their interaction in the symbiotic association.This manuscript is a report of initial studies on the characterization of the Azolla-Anabaena azollae symbiotic relationship. We describe the morphology, a method of freeing the fronds of the symbiotic alga, and isolation procedures devised to fractionate Azolla-Anabaena azollae for metabolic studies. The companion publication deals with aspects of acetylene reduction (nitrogenase activity) (25).

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“…Structures similar in appearance to the red algal phycobilisomes were evident in electron micrographs of the blue-green endosymbionts of Glaucocystis nostochinearum and Cyanophora paradoxa (5,31). Subsequently, observations of structures presumed to be phycobilisomes have been reported for the free-living blue-green algae Gloeocapsa alpicola (8), Tolypothrix tenuis, and Fremyella diplosiphon (18); Anacystis nidulans (14,41); Synechococcus lividus (11,12,28); and Aphanizomenon flos-aquae, Arthrospira Jenneri, Microcoleus vaginatus, Nostoc muscorum, Spirulina major, Symploca muscorum, and Tolypothrix distorta (41).…”

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confidence: 99%

Phycobilisomes in Blue-Green Algae

Wildman

Bowen

1974

J Bacteriol

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Fifteen species of freshwater blue-green algae, including unicellular, filamentous, and colonial forms, were subjected to a variety of fixatives, fixation conditions, and stains for comparison of the preservation of phycobilisomes. Absorption spectra of the corresponding in vivo and released photosynthetic pigments, in 10 of the species that were maintained in culture, demonstrated the presence of phycocyanin in all 10 species and phycoerythrin in only 2 of them. Spectroscope and electron microscope evidence was obtained for localization of phycobiliproteins in phycobilisomes of Nostoc muscorum. Phycobilisomes were observed in all species examined in situ, strengthening the hypothesis that phycobilisomes are common to all phycobiliprotein-containing photosynthetic blue-green algae. 866 on July 31, 2020 by guest

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Fixation of phycobiliproteins to photosynthetic membranes by glutaraldehyde

Cited by 30 publications

References 19 publications

FLUORESCENCE INDUCTION IN THE RED ALGA PORPHYRIDIUM CRUENTUM

FLUORESCENCE INDUCTION IN THE RED ALGA PORPHYRIDIUM CRUENTUM

The Azolla, Anabaena azollae Relationship

Phycobilisomes in Blue-Green Algae

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