Fixation of Ejaculated Spermatozoa for Electron Microscopy

Stefanini, Mario; Martino, C. De; Zamboni, Luciano

doi:10.1038/216173a0

Cited by 1,373 publications

(497 citation statements)

References 7 publications

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“…Briefly, 5-mdiameter frozen serial sagittal or coronal sections were cut with a cryostat and immediately placed on poly-L-lysine-precoated slides (Sigma, St. Louis, Mo.). After air drying for 24 h, cryostat sections were fixed in cold Zamboni's fixative solution (54) and acetone. An avidin-biotin complex technique was used.…”

Section: Methodsmentioning

confidence: 99%

CD8⁺T Cells Mediate Recovery andImmunopathology in West Nile VirusEncephalitis

Wang

Lobigs

Lee

et al. 2003

J Virol

255

206

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Section: Methodsmentioning

confidence: 99%

CD8⁺T Cells Mediate Recovery andImmunopathology in West Nile VirusEncephalitis

Wang

Lobigs

Lee

et al. 2003

J Virol

255

206

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“…Salivary glands from 15 insects were transferred to Zamboni's fixative solution (Stefanini et al, 1967) and dehydrated in ethanol series, embedded in glycol metacrylate historesin JB4 (Polyscience) and cut in 5 µm thin slices for analysis under light microscope. Sections were stained with haematoxylin and eosin, or submitted to the following histochemical test: mercurybromophenol blue for total proteins, PAS for neutral polysaccharides and glycoconjugates and Nile blue for acid lipids (Pearse, 1980).…”

Section: Morphologymentioning

confidence: 99%

Biochemical and morphological aspects of salivary glands of the predator Brontocoris tabidus (Heteroptera: Pentatomidae)

Azevedo

Zanúncio

Martins

et al. 2007

Braz. arch. biol. technol.

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The aim of this work was to study the feeding habits of the predator Brontocoris tabidus (Heteroptera: Pentatomidae) analyzing the salivary gland complex (SGC). The SGC was dissected and subjected to histological analyses and biochemical assays. Results showed that a pair of bilobed principal salivary glands and one pair of tubular accessory salivary glands form the SGC and different parts of salivary gland synthesizes similar substances.Lipases, α-amylase and trypsin-like enzymes were detected at both lobes of the principal salivary gland. These data together with observations related to the predator's diet suggested that it could be considered an obligate zoophytophagous.

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“…Then the mixture was transfered to the cellulose tubes, and dialyzed against distilled wtaer at 4°C for 24 to 48 hr. The fixatives used were; 1) 10% buffered formalin, 2) Zamboni's solution (19,25), 3) 100% ethanol, 4) 100% xylol, and 5) PBS (control). Before dialysis xylol-treated solution was immersed in ethanol to remove xylol.…”

Section: Methodsmentioning

confidence: 99%