Fixation of CO2 from ethanol fermentation for succinic acid production in a dual-chamber bioreactor system

Advances in succinic acid production: the enhancement of CO2 fixation for the carbon sequestration benefits

“…Overexpression of carbonic anhydrase (CA) and PEPC in E. coli DC1515 ( ΔpflA , ΔldhA , and ΔptsG400 ) increased SA production from 0.750 to 16.3 g/L, and CFR increased 21.8-fold, which was 0.127 g/L/h ( Wang et al, 2015 ; Huang et al, 2019 ). Additionally, overexpression of CA and PCK in Corynebacterium acetoacidophilum ( ΔldhA ) increased SA production from 24.0 to 27.9 g/L and CFR from 0.299 to 0.347 g/L/h ( Qian and Zheng, 2023 ). Notably, the direct substrate of carboxylases is not CO 2 but HCO 3 − ( Craveiro et al, 2022 ; Zaidi et al, 2022 ) .…”

Carbonic anhydrase assisted acidogenic fermentation of forest residues for low carbon hydrogen and volatile fatty acid production: enhanced in situ CO2 reduction and microbiological analysis

“…26,27 As such, CA has the potential to mitigate CO 2 levels in bioconversion processes resulting in elevated levels of CO 2 uptake by the microbes or enzymes and providing a viable carbon supply for the production of high-added value products, such as biofuels and chemicals. [28][29][30][31] In a study pertaining to ethanol fermentation, the use of CA in situ during fermentation was found to enhance the production of ethanol by 20%. 28 In the present study, we evaluated the effect of supplementation of CA derived from Desulfovibrio vulgaris to the acidogenic fermentation process towards biohydrogen and volatile fatty acids production from forest residue hydrolysates derived through stem explosion pretreatment.…”

“…[28][29][30][31] In a study pertaining to ethanol fermentation, the use of CA in situ during fermentation was found to enhance the production of ethanol by 20%. 28 In the present study, we evaluated the effect of supplementation of CA derived from Desulfovibrio vulgaris to the acidogenic fermentation process towards biohydrogen and volatile fatty acids production from forest residue hydrolysates derived through stem explosion pretreatment. Specifically, this study provides an insight into the intricate mechanisms of AF towards biohydrogen and volatile fatty acids in the presence of CA, investigating its potential role in regulating the pH levels, while concurrently utilizing the acidogenic CO 2 within the reactor supporting its mitigation.…”

“…26,27 As such, CA has the potential to mitigate CO 2 levels in bioconversion processes resulting in elevated levels of CO 2 uptake by the microbes or enzymes and providing a viable carbon supply for the production of high-added value products, such as biofuels and chemicals. 28–31 In a study pertaining to ethanol fermentation, the use of CA in situ during fermentation was found to enhance the production of ethanol by 20%. 28…”

“…28–31 In a study pertaining to ethanol fermentation, the use of CA in situ during fermentation was found to enhance the production of ethanol by 20%. 28…”