Five-wavelength optical-resolution photoacoustic microscopy of blood and lymphatic vessels

Liu, Chao; Chen, Jiangbo; Zhang, Yachao; Zhu, Jingyi; Wang, Lidai

doi:10.1117/1.ap.3.1.016002

Cited by 62 publications

(46 citation statements)

References 51 publications

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“…1a). The laser consists of a nanosecond 532-nm pump laser (VPFL-G-20, Spectra-Physics) and a ber-based stimulated Raman scattering (SRS) shifter 19 . The pump laser operates at 4 kHz with 7-ns pulse width.…”

Section: Methodsmentioning

confidence: 99%

“…High detection sensitivity is not only important for high-quality imaging, but also enables the detection of more low-absorbing chromophores at a lower concentration, reduces photobleaching, toxicity, or perturbation, and allows for more choices of low-cost low-power lasers at a broader spectrum. For instance, in ophthalmologic examination 16 , low laser energy is preferred for more safety and comfort; in high-speed OR-PAM, increased pulse repetition rates (PRR) limits the laser pulse energy [17][18][19][20] ; wearable or portable OR-PAM prefers lightweight low-power lasers 21 ; long-term monitoring of pharmacokinetics or hemodynamics requires low-dose imaging to reduce photobleaching or phototoxicity.…”

Section: Introductionmentioning

confidence: 99%

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Super-low-dose functional and molecular photoacoustic microscopy

Zhang

CHEN

ZHANG

et al. 2022

Preprint

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Photoacoustic microscopy can image various biological molecules and nano-agents in vivo via low-scattering ultrasonic sensing. Insufficient sensitivity has been a long-standing obstacle for imaging of many low-absorbing chromophores with less photobleaching or toxicity, reduced perturbation to delicate organs, and more choices of lasers. Here, we present a multi-spectral super-low-dose photoacoustic microscopy (SLD-PAM) that is up to ~ 33-times more sensitive than the state of the art. We collaboratively innovate the photoacoustic probe design and implement a spectral-spatial filter. The new probe collects 300% more acoustic signals, and the filter further improves the signal-to-noise ratio by 200%~1100% across all optical wavelengths. SLD-PAM can visualize microvessels and quantify oxygen saturation (sO2) in vivo with ~ 1% of the maximum permissible exposure, dramatically reducing any possible phototoxicity or perturbation, especially in imaging of the eye and the brain. Capitalizing on the high sensitivity, we, for the first time, implement direct imaging of deoxyhemoglobin (Deoxy-Hb) concentration without spectral unmixing, avoiding wavelength-dependent and computational noises. We show that super-low-dose molecular imaging can reduce photobleaching by at least 85%. We also demonstrate SLD-PAM achieves similar molecular imaging quality using 80% fewer contrast agents, which greatly reduces potential biotoxicity. SLD-PAM enables the use of more low-absorbing nano-agents, small molecules, and genetically-encoded biomarkers, as well as more low-power light sources at wider spectra. We believe SLD-PAM offers a powerful tool for anatomical, functional, and molecular imaging.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Super-low-dose functional and molecular photoacoustic microscopy

Zhang

CHEN

ZHANG

et al. 2022

Preprint

Self Cite

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“…At the output end of the fiber multiwavelength spectral peaks are generated through stimulated Raman scattering (SRS). SRS efficiency could be affected by temperature and airflow changes, and random errors and drift in pulse energy 24 . To improve the stability of the SRS peaks, the fiber was kept in a temperature-controlled unit to isolate the airflow and the temperature 25 .…”

Section: System Architecturementioning

confidence: 99%

In-Vivo Functional and Structural Retinal Imaging Using Multiwavelength Photoacoustic Remote Sensing Microscopy and Optical Coherence Tomography

Hosseinaee¹,

Pellegrino²,

Abbasi³

et al. 2021

Preprint

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A multiwavelength photoacoustic remote sensing (PARS) microscope is combined with swept-source optical coherence tomography and applied to in-vivo, non-contact retinal imaging. Building on the proven strength of multiwavelength PARS imaging, the system is applied for estimating retinal oxygen saturation in the rat eye. The capability of the technology is demonstrated by imaging both microanatomy and the microvasculature of the retina in-vivo. To our knowledge, this is the first time a non-contact photoacoustic imaging technique is employed for in-vivo oxygen saturation measurement in the retina.

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“…As an emerging biomedical imaging technique, optical-resolution photoacoustic microscopy (OR-PAM) has a sub-cellular resolution, rich optical absorption contrasts, and label-free imaging ability [ [1] , [2] , [3] , [4] , [5] , [6] , [7] , [8] , [9] , [10] , [11] , [12] ]. Fast speed is of great importance for improving throughput and the study of physiological or pathological changes in vivo, such as brain activities, drug responses, and tumor metastasis processes, et al [ [13] , [14] , [15] , [16] , [17] ].…”

Section: Introductionmentioning

confidence: 99%

Dual-foci fast-scanning photoacoustic microscopy with 3.2-MHz A-line rate

et al. 2021

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We report fiber-based dual-foci fast-scanning OR-PAM that can double the scanning rate without compromising the imaging resolution, the field of view, and the detection sensitivity. To achieve fast scanning speed, the OR-PAM system uses a single-axis water-immersible resonant scanning mirror that can confocally scan the optical and acoustic beams at 1018 Hz with a 3-mm range. Pulse energies of 45∼100-nJ are sufficient for acquiring vascular and oxygen-saturation images. The dual-foci method can double the B-scan rate to 2036 Hz. Using two lasers and stimulated Raman scattering, we achieve dual-wavelength excitation on both foci, and the total A-line rate is 3.2-MHz. In in vivo experiments, we inject epinephrine and monitor the hemodynamic and oxygen saturation response in the peripheral vessels at 1.7 Hz over 2.5 × 6.7 mm 2 . Dual-foci OR-PAM offers a new imaging tool for the study of fast physiological and pathological changes.

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Five-wavelength optical-resolution photoacoustic microscopy of blood and lymphatic vessels

Cited by 62 publications

References 51 publications

Super-low-dose functional and molecular photoacoustic microscopy

Super-low-dose functional and molecular photoacoustic microscopy

In-Vivo Functional and Structural Retinal Imaging Using Multiwavelength Photoacoustic Remote Sensing Microscopy and Optical Coherence Tomography

Dual-foci fast-scanning photoacoustic microscopy with 3.2-MHz A-line rate

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