Five Proteins of Laodelphax striatellus Are Potentially Involved in the Interactions between Rice Stripe Virus and Vector

Li, Shuo; Xiong, Renci; Wang, Xifeng; Zhou, Yijun

doi:10.1371/journal.pone.0026585

Cited by 36 publications

(42 citation statements)

References 49 publications

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“…In whitefly B. tabaci , GroEL produced by Wolbachia do not interact with TYLCV vectored by whitefly . Li et al used a virus overlay assay of protein blots to investigate RSV‐SBPH interactions, and did not find the binding between RSV particles and any GroEL. In addition, the total amount of other endosymbionts was fewer, even if their GroEL could interact with RSV/RBSDV, the effects on virus transmission for this vector might be weak, even negligible.…”

Section: Discussionmentioning

confidence: 99%

Bacterial microbiota in small brown planthopper populations with different rice viruses

Zhou

Chen

et al. 2017

J Basic Microbiol

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The small brown planthopper (SBPH) is an important virus vector, transmitting Rice stripe virus (RSV), and Rice black-streaked dwarf virus (RBSDV). Insect symbionts play an essential role in the insect fitness, however, it is still unclear about their contributions to viral transmission by SBPH. Here, we investigated endosymbiont communities in non-viruliferous, RSV-infected, and RBSDV-infected SBPH populations using Illumina 16S rRNA gene MiSeq sequencing. In total, 281,803 effective sequences of the 16S rRNA gene were generated from different samples. Sequence analysis revealed the percentages of these bacterial groups in different SBPH populations on several taxonomic levels ranging from phyla to genera. The extremely consistent bacterial diversity and abundance indicated that RSV or RBSDV infection did not affect the composition and abundance of symbionts in SBPH. It was notable that Wolbachia was dominant in all populations. The symbiosis between Wolbachia and SBPH might be potentially studied and utilized to control pest SBPH in the future.

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Section: Discussionmentioning

confidence: 99%

Bacterial microbiota in small brown planthopper populations with different rice viruses

Zhou

Chen

et al. 2017

J Basic Microbiol

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“…The protein encoded by vcRNA 4 was identified as the RSV movement protein (MP; Xiong et al, 2008). RSV is transovarially transmitted by small brown planthopper (SBPH), Laodelphax striatellus , in a circulative-propagative manner (Falk and Tsai, 1998; Li et al, 2011). After RSV infection, rice plants often show chlorotic stripes in the newly expanded leaves, and the stripes progress into pale streaks in infected plant leaves.…”

Section: Introductionmentioning

confidence: 99%

Role of rice stripe virus NSvc4 in cell-to-cell movement and symptom development in Nicotiana benthamiana

Xu¹

2012

Front. Plant Sci

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Our previous work has demonstrated that the NSvc4 protein of Rice stripe virus (RSV) functions as a cell-to-cell movement protein. However, the mechanisms whereby RSV traffics through plasmodesmata (PD) are unknown. Here we provide evidence that the NSvc4 moves on the actin filament and endoplasmic reticulum network, but not microtubules, to reach cell wall PD. Disruption of cytoskeleton using different inhibitors altered NSvc4 localization to PD, thus impeding RSV infection of Nicotiana benthamiana. Sequence analyses and deletion mutagenesis experiment revealed that the N-terminal 125 amino acids (AAs) of the NSvc4 determine PD targeting and that a transmembrane domain spanning AAs 106–125 is critical for PD localization. We also found that the NSvc4 protein can localize to chloroplasts in infected cells. Analyses using deletion mutants revealed that the N-terminal 73 AAs are essential for chloroplast localization. Furthermore, expression of NSvc4 from a Potato virus X (PVX) vector resulted in more severe disease symptoms than PVX alone in systemically infected N. benthamiana leaves. Expression of NSvc4 in Spodoptera frugiperda 9 cells did not elicit tubule formation, but instead resulted in punctate foci at the plasma membrane. These findings shed new light on our understanding of the movement mechanisms whereby RSV infects host plants.

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“…Identification of virus receptors in their insect vectors has been unsuccessful for circulative viruses as a whole. However, many insect proteins that bind virus particles in vitro have been identified primarily using far-Western blotting (8,9,12,13). Two proteins isolated from the head of the aphid Sitobion avenae, SaM35 and SaM50, bound to barley yellow dwarf virus-MAV (BYDV-MAV) particles and were thought to be potential receptors in the ASG (8).…”

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confidence: 99%

In Vitro Evidence Supports Membrane Alanyl Aminopeptidase N as a Receptor for a Plant Virus in the Pea Aphid Vector

Linz

Liu

Chougule

et al. 2015

J Virol

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Insect-borne plant viruses cause significant agricultural losses and jeopardize sustainable global food production. Although blocking plant virus transmission would allow for crop protection, virus receptors in insect vectors are unknown. Here we identify membrane alanyl aminopeptidase N (APN) as a receptor for pea enation mosaic virus (PEMV) coat protein (CP) in the gut of the pea aphid, Acyrthosiphon pisum, using a far-Western blot method. Pulldown and immunofluorescence binding assays and surface plasmon resonance were used to confirm and characterize CP-APN interaction. PEMV virions and a peptide comprised of PEMV CP fused to a proline-rich hinge (-P-) and green fluorescent protein (CP-P-GFP) specifically bound to APN. Recombinant APN expressed in Sf9 cells resulted in internalization of CP-P-GFP, which was visualized by confocal microscopy; such internalization is an expected hallmark of a functional gut receptor. Viruses that infect crop plants restrict our ability to consistently produce high yields from agricultural crops. Many of these viruses are transmitted to plants by pestiferous insects, with aphids transmitting nearly half of the 600 insect-borne plant viruses. Aphids therefore represent a significant threat to global agriculture (1). Viruses in the family Luteoviridae are phloem-restricted RNA viruses transmitted exclusively by aphids and cause disease in multiple food crops (2). Luteovirids are transmitted in a circulative and persistent manner which involves specific molecular interactions between the virus and the aphid (3). For this type of transmission, ingested virions cross the aphid gut and salivary gland epithelial barriers for transmission to additional plant hosts.Luteovirus-aphid interactions are mediated by the viral capsid proteins consisting of a major coat protein (CP; 22 kDa) and one minor coat protein readthrough domain (CP-RTD; 35 to 55 kDa) (3). The RTD is not required for virus particle assembly or for uptake of virus from the gut into the aphid hemocoel, but both CP and RTD are essential for aphid transmission and are the sole determinants of vector specificity (3). The virus binds to a receptor in either the midgut, hindgut, or both for transcytosis across the aphid gut epithelium and release into the hemocoel (4). A second receptor-mediated transcytosis event occurs at the accessory salivary glands (ASG) from which virus particles are secreted with saliva to inoculate the plant phloem during subsequent feedings (5). Only a fraction of the virions present in the aphid hemolymph cross into the ASG, and a threshold amount of virus in the hemocoel is required before transmission via the ASG can occur (3). Hence, impeding the binding of a plant virus to the aphid gut receptor could reduce the amount of virus present in the hemocoel to less-than-threshold levels, thereby reducing plant virus transmission. Citation Linz LB, Liu S, Chougule NP, Bonning BC. 2015. In vitro evidence supports membrane alanyl aminopeptidase N as a receptor for a plant virus in the pea aphid ve...

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Five Proteins of Laodelphax striatellus Are Potentially Involved in the Interactions between Rice Stripe Virus and Vector

Cited by 36 publications

References 49 publications

Bacterial microbiota in small brown planthopper populations with different rice viruses

Bacterial microbiota in small brown planthopper populations with different rice viruses

Role of rice stripe virus NSvc4 in cell-to-cell movement and symptom development in Nicotiana benthamiana

In Vitro Evidence Supports Membrane Alanyl Aminopeptidase N as a Receptor for a Plant Virus in the Pea Aphid Vector

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