Five new insulin-producing cell lines with differing secretory properties

Abstract

Five cell lines have been derived from a rat transplantable islet cell tumour using two different methods. The lines differ in morphology and contain and release different amounts of insulin and glucagon (insulin content, 1-90 pmol/10(6) cells; insulin release, 6-250 pmol/10(6) cells per 24 h; glucagon content, less than 0.005-35 pmol/10(6) cells; glucagon release, less than 0.05-10 pmol/10(6) cells per 24 h). All the lines responded to the presence of the secretagogues leucine (20 mmol/l) plus theophylline (5 mmol/l) by increasing the rate of release of insulin approximately twofold. A high extracellular concentration of potassium (40 mmol/l) caused a three- to tenfold calcium-dependent increase in release of insulin and a parallel release of glucagon. Increasing the concentration of glucose from 2.8 to 16.7 mmol/l did not alter the rate of insulin release by any of the cell lines.

Inhibition of a calcium‐activated, non‐selective cation channel, in a rat insulinoma cell line, by adenine derivatives

“…All experiments were performed on cells of the rat pancreatic islet cell line, CRI-Gl, which were passaged at weekly intervais and maintained in ' To whom correspondence should be addressed Dulbecco's modified Eagles medium at 37°C in a humidified atmosphere of 95% sir/5% CO2 [7].…”

Characterization of a nicotinamide–adenine dinucleotide‐dependent cation channel in the CRI‐G1 rat insulinoma cell line

“…These were grown in Dulbecco's modified Eagle's medium at 37°C in a humidified atmosphere of 95% air-5% COµ and passaged at 2-5 day intervals as previously described (Carrington, Rubery, Pearson & Hales, 1986). For electrophysiological experiments cells were plated onto 3·5 cm petri dishes (Falcon 3001) and used 1-4 days after plating.…”

Mg2+‐dependent inhibition of KATP by sulphonylureas in CRI‐G1 insulin‐secreting cells

“…Cells of the rat pancreatic islet cell line CRI-G1 were cultured and passaged at 2-5 day intervals as previously described (Carrington et al, 1986). Cells used for patch-clamp experiments were plated onto 3.5 cm Petri dishes (Falcon 3001) at a density of approximately 1.5 x 10' cells per dish.…”