Five human tumour cell lines derived from a primary squamous carcinoma of the tongue, two subsequent local recurrences and two nodal metastases

Easty, D.M.; Easty, G. C.; Carter, R L; Monaghan, Paul; Pittam, M.; James, Ted A.

doi:10.1038/bjc.1981.193

Cited by 41 publications

(11 citation statements)

References 9 publications

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“…The procedure for isolating the cell lines and the characteristics of the cells have been presented elsewhere (Easty et al, 1981;Haemmerli and Felix. 1981).…”

Section: Methodsmentioning

confidence: 99%

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Comparative chromosome analysis of nine squamous cell carcinoma lines from tumors of the head and neck

Hauser-Urfer¹,

Stauffer²

1985

Cytogenet Genome Res

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The chromosomes of nine cell lines from human squamous cell carcinomas of the head and neck were examined. Five of the lines were derived from patients who had received chemotherapy and radiotherapy, one line was derived from a lymph-node metastasis from one of these patients, and three lines were from patients who had not been treated. The cell lines from the untreated patients had lower modal chromosome numbers than those of all but one of the lines from the treated patients. A structural abnormality involving chromosome 1 with breakpoints located in p13, p22, q21, or q32 was found in all cases. The NRAS and SK oncogenes are located in or near some of these segments. In four of the cell lines the nucleolus organizer regions (NORs) were 10–30 times larger than the NORs of the normal human chromosomes. Double minutes (DM) and homogeneously staining regions (HSR) were found in four of the lines.

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“…The procedure for isolating the cell lines and the characteristics of the cells have been presented elsewhere (Easty et al, 1981;Haemmerli and Felix. 1981).…”

Section: Methodsmentioning

confidence: 99%

“…These nine cell lines differ from one another in their invasiveness, ability to destroy tissue, morphology, ultrastructure, and patterns of growth (Easty et al 1981;Haemmerli and Felix. 1981;Haemmerli and Strauli, 1981).…”

mentioning

confidence: 99%

Comparative chromosome analysis of nine squamous cell carcinoma lines from tumors of the head and neck

Hauser-Urfer¹,

Stauffer²

1985

Cytogenet Genome Res

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“…The derivation and routine culture of cell line HN-5 has been described elsewhere together with its establishment as a transplantable xenograft in immunosuppressed mice. 1,52, 60 Primary epidermal keratinocyte cultures were prepared from skin derived from mastectomies and reduction mammoplasties. Adipose tissue was first dissected away and small (8 mm) cubes of skin were incubated in DMEM containing 0.025 per cent collagenase overnight at 37°C to separate the epidermis.…”

Section: Keratinocyte Cell Culture and Cell Linesmentioning

confidence: 99%

Monoclonal antibodies specific for subsets of epidermal keratins: Biochemical and immunocytochemical characterization—applications in pathology and cell culture

Knight

Gusterson

Jones

et al. 1985

The Journal of Pathology

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Keratin composition has been widely used as a biochemical marker of differentiation in normal epithelia, cell culture systems and tumours of epithelial tissues. We have been developing a model system for the study of human squamous epithelial cell differentiation, and among a panel of monoclonal antibodies we have generated for analysing this system are two antibodies recognizing subsets of epidermal keratins. The two antibodies, designated LICR-LON-16a and LICR-LON-29b, were raised to the human squamous carcinoma cell line LICR-LON-HN-5, and we describe here their biochemical and immunocytochemical characterization. Antibody 16a reacts with only epidermal basal cells in normal human skin and shows specificity for the 45 and 46 kdalton keratins. Antibody 29b stains all living layers of the epidermis, and reacts with a broad range of ketain polypeptides, (45-56 kdaltons) in immunoblotting analyses. We have investigated the alterations of cellular staining that occur in chronic hyperproliferative skin diseases and carcinomas and compared this with the staining of multilayered cultures of normal keratinocytes and the HN-5 cell line. We show that in squamous cell carcinomas and in HN-5 cell xenografts 16a and 29b stain only the well-differentiated cell types. Furthermore we found that the basal cell specificity of 16a was lost in all of the hyperproliferative skin lesions examined including psoriasis and eczema. This transition to suprabasal staining pattern was also seen in the cultures of normal keratinocytes and HN-5 cells. We conclude that aberrant keratin synthesis or abnormal post-translational processing of keratins associated with an increased rate of cell turnover could account for the altered expression of the epitope recognized by antibody 16a.

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“…The three cell lines derived from human squamous carcinomas of tongue [LICR(Lond)-HN 1 and LICR(Lond)- HN 6] and larynx [LICR(Lond)-HN 2] have been described (26,27).…”

Section: Methodsmentioning

confidence: 99%

Calcium-binding proteins in human carcinoma cell-lines

Pfyffer¹,

Haemmerli²,

Sträuli³

et al. 1984

Cell Calcium

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Five human tumour cell lines derived from a primary squamous carcinoma of the tongue, two subsequent local recurrences and two nodal metastases

Cited by 41 publications

References 9 publications

Comparative chromosome analysis of nine squamous cell carcinoma lines from tumors of the head and neck

Comparative chromosome analysis of nine squamous cell carcinoma lines from tumors of the head and neck

Monoclonal antibodies specific for subsets of epidermal keratins: Biochemical and immunocytochemical characterization—applications in pathology and cell culture

Calcium-binding proteins in human carcinoma cell-lines

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