Five-dimensional two-photon volumetric microscopy of in-vivo dynamic activities using liquid lens remote focusing

Tehrani, Kayvan Forouhesh; Latchoumane, Charles; Southern, William M.; Pendleton, Emily G.; Maslesa, Ana; Karumbaiah, Lohitash; Call, Jarrod A.; Mortensen, Luke J.

doi:10.1364/boe.10.003591

Cited by 30 publications

(14 citation statements)

References 39 publications

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“…A Ti:Sapphire source (Coherent Ultra II) tuned to 932 nm wavelength with 140 fs pulsed laser light was used for SHG imaging, and a 466/5 nm filter (Semrock) was used for light collection. Details of the microscope setup are explained elsewhere [41]. Because the SHG signal is proportional to the index of refraction of the tissue, we can use stacks of 3-dimensional images to model the tissue and hence the wavefront of the propagated light.…”

Section: Correlative Phase Accumulation Ray Tracingmentioning

confidence: 99%

In situ measurement of the isoplanatic patch for imaging through intact bone

Tehrani

Koukourakis

Czarske

et al. 2020

Journal of Biophotonics

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Wavefront-shaping (WS) enables imaging through scattering tissues like bone, which is important for neuroscience and bone-regeneration research. WS corrects for the optical aberrations at a given depth and field-of-view (FOV) within the sample; the extent of the validity of which is limited to a region known as the isoplanatic patch (IP). Knowing this parameter helps to estimate the number of corrections needed for WS imaging over a given FOV. In this paper, we first present direct transmissive measurement of murine skull IP using digital optical phase conjugation based focusing. Second, we extend our previously reported phase accumulation ray tracing (PART) method to provide in-situ in-silico estimation of IP, called correlative PART (cPART). Our results show an IP range of 1 to 3 μm for mice within an age range of 8 to 14 days old and 1.00 ± 0.25 μm in a 12-week old adult skull. Consistency between the two measurement approaches indicates that cPART can be used to approximate the IP before a WS experiment, which can be used to calculate the number of corrections required within a given field of view.

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Section: Correlative Phase Accumulation Ray Tracingmentioning

confidence: 99%

In situ measurement of the isoplanatic patch for imaging through intact bone

Tehrani

Koukourakis

Czarske

et al. 2020

Journal of Biophotonics

Self Cite

View full text Add to dashboard Cite

show abstract

“…A Ti:Sapphire source (Coherent Ultra II) tuned to 940 nm wavelength with 140 fs pulsed laser light was used for SHG imaging, and a 466/5 nm filter (Semrock) was used for light collection. Details of the microscope setup are explained elsewhere [41]. Because the SHG signal is proportional to the index of refraction of the tissue, we can use stacks of 3-dimensional images to model the tissue and hence the wavefront of the propagated light.…”

Section: Correlative Phase Accumulation Ray Tracingmentioning

confidence: 99%

In situ measurement of the isoplanatic patch for imaging through intact bone

Tehrani

Koukourakis

Czarske

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Wavefront-shaping (WS) enables imaging through scattering tissues like bone, which is important for neuroscience and bone-regeneration research. WS corrects for the optical aberrations at a given depth and field-of-view (FOV) within the sample; the extent of the validity of which is limited to a region known as the isoplanatic patch (IP). Knowing this parameter helps to estimate the number of corrections needed for WS imaging over a given FOV. In this paper, we first present direct transmissive measurement of murine skull IP using digital optical phase conjugation (DOPC) based focusing. Second, we extend our previously reported Phase Accumulation Ray Tracing (PART) method to provide in-situ in-silico estimation of IP, called correlative PART (cPART). Our results show an IP range of 1-3 μm for mice within an age range of 8-14 days old and 1.00+/-0.25 μm in a 12-week old adult skull. Consistency between the two measurement approaches indicates that cPART can be used to approximate the IP before a WS experiment, which can be used to calculate the number of corrections required within a given field of view.

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“…However, they cannot achieve continuous zoom change. For example, a five-dimensional microscopy using liquid lens is proposed and able to scan volumes rapidly and reproducibly 21 . This microscopy has fast scan speed however it cannot zoom continuously.…”

Section: Introductionmentioning

confidence: 99%

Large zooming range adaptive microscope employing tunable objective and eyepiece

Kuang

Yuan

Wang

et al. 2020

Sci Rep

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The conventional microscope has discrete magnification and slow response time in zoom process, which is difficult to capture the dynamic activity of the live specimen. We demonstrate an adaptive microscope employing a tunable objective and a tunable eyepiece with large zooming range. The tunable objective consists of three glass lenses and four electrowetting liquid lenses. The tunable eyepiece consists of an achromatic eyepiece and an electrowetting liquid lens. The focal point between the objective and the eyepiece is designed to be tunable, which are controlled by voltages. Thus, the tuning range is relatively large. We fabricate the adaptive microscope and observe the specimen. In the experiment, the magnification of the microscope changes continuously from ~ 59.1 × to ~ 159.2 × , and the largest numerical aperture is ~ 0.212. The tunable eyepiece can release the back focal length of the tunable objective, which increases the zoom range of the microscope. No mechanical movement is required and the aberrations can be corrected over a wide wavelength range. Thus, the proposed adaptive microscope has a potential application in biological research and clinical medical examination. Microscopes play an important role in scientific research and production, such as physiological applications 1 , biomedical engineering 2 and microfabrication 3. For targets of different sizes, microscopes need different magnifications. Besides, to observe large area of cells and a zoom-in area with high resolution, the higher requirements of real-time observation and continuous zoom change on microscopes emerges 4. The conventional microscopes can change magnifications by manually converting objectives. But its magnifications are not continuous and the conversion operation introduces sample vibration. One of the solutions for continuous zoom change is to use the mechanical or optical compensation system driven by mechanical movement 5,6. However, such compensation systems are bulky and sample vibration is still an issue due to the mechanical movement. Besides the slow zoom speed affects the real-time observation. Fortunately, the liquid lenses have changed the traditional lens systems 7. Due to lightweight, low power consumption and fast response speed, liquid lenses have important applications in imaging 8-10 , display 11 , and communication 12. Moreover, liquid lenses are wildly used in microscopy as focusing component for axial scanning 13-16 , increasing the depth-of-field 17,18 and autofocusing 19,20. However, they cannot achieve continuous zoom change. For example, a five-dimensional microscopy using liquid lens is proposed and able to scan volumes rapidly and reproducibly 21. This microscopy has fast scan speed however it cannot zoom continuously. A adaptive microscope objective using liquid lens is proposed 22. The magnification of the adaptive microscope objective tune from ~ 7.8 × to ~ 13.2 × , but its zoom range is very limited due to fixed back focal length (BFL). Therefore, it is still urgent to study microscopes with larg...

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Five-dimensional two-photon volumetric microscopy of in-vivo dynamic activities using liquid lens remote focusing

Cited by 30 publications

References 39 publications

In situ measurement of the isoplanatic patch for imaging through intact bone

In situ measurement of the isoplanatic patch for imaging through intact bone

In situ measurement of the isoplanatic patch for imaging through intact bone

Large zooming range adaptive microscope employing tunable objective and eyepiece

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