Five Days Periodic Fasting Elevates Levels of Longevity Related Christensenella and Sirtuin Expression in Humans

Lilja, Stephanie; Stoll, Carina; Krammer, Ulrike; Hippe, Berit; Duszka, Kalina; Debebe, Tewodros; Höfinger, Ingrid; König, Jürgen; Pointner, Angelika; Haslberger, Alexander G.

doi:10.3390/ijms22052331

Cited by 30 publications

(18 citation statements)

References 49 publications

(49 reference statements)

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“…The transcriptomic effects of IF in humans are still under investigation (studies are underway), but initial results from studies of individual genes indicate that IF (5 days of periodic fasting) increases transcriptomic signatures of nutrient and energy sensing/oxidative phosphorylation gene expression including AMP-activated protein kinase (AMPK), Forkhead box protein 01 (FOXO1), Sirtuin 1 (SIRT1), and Sirtuin 3 (SIRT 3). These genes/proteins are key players in cellular regulation pathways and centrally implicated stress responses, cardiometabolic/systemic health, and longevity ( 35 ). Another study ( 36 ) confirmed these results with data showing that IF contributes to a marginal increase in SIRT3 gene expression, which was associated with a decrease in plasma insulin levels.…”

Section: Intermittent Fastingmentioning

confidence: 99%

Transcriptomic Effects of Healthspan-Promoting Dietary Interventions: Current Evidence and Future Directions

Wahl

LaRocca

2021

Front. Nutr.

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Aging is the greatest risk factor most diseases, including cardiovascular disorders, cancers, diabetes, and neurodegeneration, but select nutritional interventions may profoundly reduce the risk for these conditions. These interventions include calorie restriction, intermittent fasting, protein restriction, and reducing intake of certain amino acids. Certain ad libitum diets, including the Mediterranean, Finnish Geriatric Intervention Study to Prevent Cognitive Impairment and Disability, and Okinawan diets also promote healthy aging. Evidence indicates that these dietary strategies influence aging and healthspan by acting on the biological “hallmarks of aging” and especially upstream nutrient sensing pathways. Recent advances in “omics” technologies, including RNA-sequencing (transcriptomics), have increased our understanding of how such nutritional interventions may influence gene expression related to these biological mediators of aging, primarily in pre-clinical studies. However, whether these effects are also reflected in the human transcriptome, which may provide insight on other downstream/related cellular processes with aging, is an emerging topic. Broadly, the investigation of how these nutritional interventions influence the transcriptome may provide novel insight into pathways associated with aging, and potential targets to treat age-associated disease and increase healthspan. Therefore, the purpose of this mini review is to summarize what is known about the transcriptomic effects of key dietary/nutritional interventions in both pre-clinical models and humans, address gaps in the literature, and provide insight into future research directions.

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Section: Intermittent Fastingmentioning

confidence: 99%

Transcriptomic Effects of Healthspan-Promoting Dietary Interventions: Current Evidence and Future Directions

Wahl

LaRocca

2021

Front. Nutr.

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“…The fecal samples were collected using INTEST.pro (Biomes Laboratory, Wildau, Germany) and then measured and analyzed by BIOMES laboratory (Wildau, Germany), using 16s rRNA NGS for fecal bacterial abundance, according to Lilja et al, 2021 [ 53 ].…”

Section: Methodsmentioning

confidence: 99%

A Polyclonal Aptamer Library for the Specific Binding of the Gut Bacterium Roseburia intestinalis in Mixtures with Other Gut Microbiome Bacteria and Human Stool Samples

Zhang

Krämer

et al. 2022

IJMS

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Roseburia intestinalis has received attention as a potential probiotic bacterium. Recent studies have demonstrated that changes in its intestinal abundance can cause various diseases, such as obesity, enteritis and atherosclerosis. Probiotic administration or fecal transplantation alter the structure of the intestinal flora, offering possibilities for the prevention and treatment of these diseases. However, current monitoring methods, such as 16S rRNA sequencing, are complex and costly and require specialized personnel to perform the tests, making it difficult to continuously monitor patients during treatment. Hence, the rapid and cost-effective quantification of intestinal bacteria has become an urgent problem to be solved. Aptamers are of emerging interest because their stability, low immunogenicity and ease of modification are attractive properties for a variety of applications. We report a FluCell-SELEX polyclonal aptamer library specific for R. intestinalis isolated after seven evolution rounds, that can bind and label this organism for fluorescence microscopy and binding assays. Moreover, R. intestinalis can be distinguished from other major intestinal bacteria in complex defined mixtures and in human stool samples. We believe that this preliminary evidence opens new avenues towards aptamer-based electronic biosensors as new powerful and inexpensive diagnostic tools for the relative quantitative monitoring of R. intestinalis in gut microbiomes.

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“…For mtDNA levels quantification, DNA was extracted from intestinal mucosa samples using QIAamp Fast DNA Tissue Kit (Qiagen, Germany). The quantification was performed as described previously [ 25 ]. Bacteria DNA levels were assessed by qRT-PCR following fecal DNA extraction (innuPREP Stool DNA Kit, Analytik Jena, Germany).…”

Section: Methodsmentioning

confidence: 99%

A Comparison of the Impact of Restrictive Diets on the Gastrointestinal Tract of Mice

et al. 2022

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The rate of gut inflammatory diseases is growing in modern society. Previously, we showed that caloric restriction (CR) shapes gut microbiota composition and diminishes the expression of inflammatory factors along the gastrointestinal (GI) tract. The current project aimed to assess whether prominent dietary restrictive approaches, including intermittent fasting (IF), fasting-mimicking diet (FMD), and ketogenic diet (KD) have a similar effect as CR. We sought to verify which of the restrictive dietary approaches is the most potent and if the molecular pathways responsible for the impact of the diets overlap. We characterized the impact of the diets in the context of several dietary restriction-related parameters, including immune status in the GI tract; microbiota and its metabolites; bile acids (BAs); gut morphology; as well as autophagy-, mitochondria-, and energy restriction-related parameters. The effects of the various diets are very similar, particularly between CR, IF, and FMD. The occurrence of a 50 kDa truncated form of occludin, the composition of the microbiota, and BAs distinguished KD from the other diets. Based on the results, we were able to provide a comprehensive picture of the impact of restrictive diets on the gut, indicating that restrictive protocols aimed at improving gut health may be interchangeable.

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Five Days Periodic Fasting Elevates Levels of Longevity Related Christensenella and Sirtuin Expression in Humans

Cited by 30 publications

References 49 publications

Transcriptomic Effects of Healthspan-Promoting Dietary Interventions: Current Evidence and Future Directions

Transcriptomic Effects of Healthspan-Promoting Dietary Interventions: Current Evidence and Future Directions

A Polyclonal Aptamer Library for the Specific Binding of the Gut Bacterium Roseburia intestinalis in Mixtures with Other Gut Microbiome Bacteria and Human Stool Samples

A Comparison of the Impact of Restrictive Diets on the Gastrointestinal Tract of Mice

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