Five BoLA-DRB3 genotypes detected in Egyptian buffalo infected with Foot and Mouth disease virus serotype O

Abstract

Foot and Mouth disease (FMD) is a contagious disease leads to economically loss in livestock production all over the world. This serious disease is caused due to the infection of the animal with a single-stranded RNA virus (FMDV). This study aimed to investigate the genetic polymorphism of BoLA-DRB3 gene in Egyptian buffalo as a candidate genetic marker included in multi-factorial process of FMD resistance/susceptibility. Also this work aimed to genetically characterization and serotyping of circulating FMD virus in Egypt during 2016.For serotyping of FMDV, RT-PCR was used for FMDV-positive samples and the results declared the presence of serotype O in all tested animals. The sequence analysis of FMDV samples revealed five different patterns for the detected serotype O which were submitted to GenBank under the accession Nos.: MG017361–MG017365.The 302-bp amplified fragments from BoLA-DRB3 exon 2 were digested with HaeIII endonuclease and the results showed that the presence of five BoLA-DRB3 genotypes, among them the genotype AA might be associated with FMD-resistance (P < 0.01). On the other hand, genotype AC could be correlated with susceptibility (P < 0.01) to FMD in Egyptian buffaloes where it was absent in resistant group. The five detected genotypes of BoLA-DRB3 exon 2 were submitted to GenBank with the accession Nos.: MF977316–MF977320. In conclusion, our findings suggested that the detection of different BoLA-DRB3 genotypes may be has a promising role for raising the resistance of Egyptian buffalo against FMDV especially serotype O which is prevalent in Egypt with preferring genotype AA.

BoLA-DRB3 Polymorphism Controls Proviral Load and Infectivity of Bovine Leukemia Virus (BLV) in Milk

“…Previous studies have shown that BoLA-DRB3 polymorphism in BLV-infected cattle is related to blood proviral load (PVL), infectivity, lymphoma, and in utero infection of calves [18,[23][24][25][26][27][28][29]. In addition to BLV, BoLA-DRB3 polymorphism is associated with infectious diseases such as bovine clinical mastitis, papillomavirus infection, herpes virus type 1 infection, and foot-and-mouth disease [30][31][32][33]. Therefore, the BoLA-DRB3 polymorphism is considered a useful gene in immunology.…”

Association of <i>BoLA-DRB3</i> with bovine leukemia virus

“…The polymorphism in this region is therefore associated with the variability in the immune responsiveness in different individuals to particular infectious diseases and economic traits such as reproduction 22) , colostrum and milk microbiota 23) , milk quality and production rate 5) . For infection disease, BoLA-DRB3 relates with mastitis 24,25) , tick-borne disease 26,27) , foot and mouth disease (FMD) 28) , bovine herpesvirus 1 (BoHV-1) 29) , bovine papillomavirus-induced bladder cancer 30) , but not relates with the bovine tuberculosis (bTB) susceptibility 31) . The association studies of BoLA-DRB3 and economic traits and disease are summarized in Table 2.…”

Whole-Genome Sequencing and Characterization of Buffalo Genetic Resources: Recent Advances and Future Challenges

“…In exon 2 of the BoLA-DRB3 gene, 302-bp-amplified fragments were digested with HaeIII endonuclease enzyme and five BoLA-DRB3 genotypes were traced and the genotype AA was correlated with FMD resistance. However, the AC genotype might be associated with FMD susceptibility in Egyptian buffaloes [ 130 ]. Furthermore, in cattle, the natural resistance to brucellosis has been associated with the (GT)13 microsatellite allele of SLC11A1 at 3′ UTR [ 131 ].…”