The objective of this work was to evaluate the potential of Urochloa brizantha cv. Marandu and Panicum maximum cv. Mombasa in phytoremediation of soil treated with 2,4-D + picloram herbicide, using Raphanus sativus Crimson Gigante as a bioindicator plant. The experiment was carried out in a greenhouse, in a completely randomized design in two stages. In the first stage the treatments were: cultivation of U. brizantha and P. maximum treated with and without the herbicide dose, with five replications. In the second stage, the treatments consisted of cultivating R. sativus in soil: free of herbicide residue; and soil contaminated with cultivation: prior to U. brizantha; P. maximum; and without previous cultivation of grass, with five replications. The units were treated with the herbicide, individually in pre-emergence, after 15 days the grasses were sown. After 50 days, forages were harvested and segregated in aerial and root parts, analyzing fresh and dry biomass (g) and height (cm). After removing phytoremediation plants, R. sativus was transplanted, evaluating visual phytotoxicity at 5, 10, 15 and 20 days after emergence (DAE) and at 20 DAE, the accumulation of green and dry matter (g), height (cm). The evaluated grasses have phytoremediation characteristics for auxinic herbicides; R. sativus can be used as a bioindicator of the herbicide 2,4-D + picloram; the evaluated period was not enough to fully remove the effects of the herbicide.