Fitorreguladores na calogênese e rizogênese em Eugenia involucrata

Golle, Diego Pascoal; Reiniger, Lia Rejane Silveira; Stefanel, Charlene Moro; Serrote, Caetano Miguel Lemos

doi:10.4336/2020.pfb.40e201901908

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…iii Means followed by the same letter in a row do not differ significantly from each other by Tukey's test (P > 0.05). E. involucrata (Golle et al 2020), among others. Although somatic embryo induction from leaf disks has been reported for some species, for cac ¸ari these explants were not responsive.…”

Section: Discussionmentioning

confidence: 99%

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Somatic Embryogenesis of Myrciaria dubia (Kunth.) McVaugh

Chagas¹,

Crane

Chagas

et al. 2023

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Myrciaria dubia (caçari or camu-camu) is a species with great relevance because of its high levels of bioactive compounds and antioxidant activity. The species is propagated mainly by seed, which leads to high genetic diversity. Producing plants that maintain their characteristics on a large scale at a low cost is a challenge for fruit species, especially in the Myrtaceae family. Therefore, this study aimed at assessing the effects of different plant growth regulators at various concentrations for the induction of somatic embryogenesis from caçari nodal segments and leaf disk explants. Two independent experiments were performed using nodal segments and leaf disks from plants grown in the greenhouse. In the first experiment, the combined effect of auxin and cytokinin at different concentrations was evaluated: 2,4-dichlorophenoxyacetic acid at 0, 1, 2, and 4 mg⋅L–1; and benzylaminopurine at 0, 0.25, 0.5, and 1 mg⋅L–1). In the second experiment, the application of different plant growth regulators (benzylaminopurine, kinetin, thidiazuron, and isopentenyl adenine) and their concentrations (0, 2, 4, and 6 mg⋅L–1) were evaluated. In both experiments, the basic culture medium was woody plant medium. Callus formation via nodal segments and leaf disks occurred in the first 30 d from cultivation and proved to be responsive to induction by 2,4-dichlorophenoxyacetic acid and benzylaminopurine. Auxin proved to be essential for somatic embryogenesis induction in nodal segments using 1 and 4 mg⋅L–1 of 2,4-dichlorophenoxyacetic acid alone. The results of this experiment will help advancing protocols for regeneration of somatic embryos and elucidating the physiological, molecular, and genetic mechanisms involved in the process of somatic embryogenesis for M. dubia. The development of an efficient protocol for in vitro clonal propagation of this species also lays the groundwork for further optimization of the system for genetic transformation.

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Section: Discussionmentioning

confidence: 99%

“…Somatic embryogenesis has been applied and documented successfully for clonal propagation of fruit-bearing species such as Acca sellowiana (Cangahuala-Inocente et al 2007Pavei et al 2018), Psidium guajava L. (Akhtar 2013;Bajpai et al 2016;Kamle et al 2014;Nasim 2010), Eugenia involucrata (Golle et al 2020) and Syzygium cumini (L.) (Naaz et al 2019).…”

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confidence: 99%