Fitness of wAlbB Wolbachia Infection in Aedes aegypti: Parameter Estimates in an Outcrossed Background and Potential for Population Invasion

Axford, Jason K.; Ross, Perran A; Yeap, Heng Lin; Callahan, Ashley G.; Hoffmann, Ary A.

doi:10.4269/ajtmh.15-0608

Cited by 109 publications

(177 citation statements)

References 36 publications

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“…The reduced hatch rates in w Mel may reflect the fact that infected males exposed to 26–37°C partly maintain the ability to cause cytoplasmic incompatibility, whereas females reared under these temperatures have lost much of their ability to restore compatibility. For w MelPop-CLA which maintained a relatively high hatch rate regardless of temperature, the higher density of this infection compared to w Mel [20, 25] may have allowed females to largely maintain compatibility even when reared under the 26–37°C regime. This requires further testing and other factors such as tissue tropism might also be involved.…”

Section: Discussionmentioning

confidence: 99%

“…Uninfected Aedes aegypti mosquitoes were collected from Townsville, Queensland, in November 2015 and maintained in a temperature controlled insectary at 26°C ± 1°C according to methods described by Axford and others [25]. Aedes aegypti with the w Mel, w MelPop-CLA and w AlbB infections of Wolbachia were derived from lines transinfected previously [19–21].…”

Section: Methodsmentioning

confidence: 99%

“…We selected 10 males and 10 females at random per tray for Wolbachia quantification. DNA extraction and Wolbachia quantification were conducted according to methods described previously [24, 25, 94]. DNA from adults with both wings removed was extracted using 150 μL of 5% Chelex 100 resin (Bio-Rad Laboratories, Hercules, CA).…”

Section: Methodsmentioning

confidence: 99%

“…Cage and field trials of the w MelPop-CLA infection demonstrate its difficulty in invading and persisting [20, 31], though the infection could have utility in population suppression programs [32, 33] due to its detrimental effect on quiescent egg viability [27, 28]. The w AlbB infection is yet to be released in the field but it has successfully invaded caged populations in the laboratory [21, 25]. …”

Section: Introductionmentioning

confidence: 99%

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Wolbachia Infections in Aedes aegypti Differ Markedly in Their Response to Cyclical Heat Stress

et al. 2017

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Aedes aegypti mosquitoes infected with Wolbachia bacteria are currently being released for arbovirus suppression around the world. Their potential to invade populations and persist will depend on interactions with environmental conditions, particularly as larvae are often exposed to fluctuating and extreme temperatures in the field. We reared Ae. aegypti larvae infected with different types of Wolbachia (wMel, wAlbB and wMelPop-CLA) under diurnal cyclical temperatures. Rearing wMel and wMelPop-CLA-infected larvae at 26–37°C reduced the expression of cytoplasmic incompatibility, a reproductive manipulation induced by Wolbachia. We also observed a sharp reduction in the density of Wolbachia in adults. Furthermore, the wMel and wMelPop-CLA infections were not transmitted to the next generation when mosquitoes were exposed to 26–37°C across all life stages. In contrast, the wAlbB infection was maintained at a high density, exhibited complete cytoplasmic incompatibility, and was transmitted from mother to offspring with a high fidelity under this temperature cycle. These findings have implications for the potential success of Wolbachia interventions across different environments and highlight the importance of temperature control in rearing.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Wolbachia Infections in Aedes aegypti Differ Markedly in Their Response to Cyclical Heat Stress

et al. 2017

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“…Similar incompatibilities in Ae . aegypti would have implications for control strategies that rely on successful mating between the release and target mosquitoes, like Wolbachia- based population replacement and suppression [18, 19], releases of sterile males [20] or males with a RIDL construct [21]. …”

Section: Introductionmentioning

confidence: 99%

The queenslandensis and the type Form of the Dengue Fever Mosquito (Aedes aegypti L.) Are Genomically Indistinguishable

et al. 2016

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BackgroundThe mosquito Aedes aegypti (L.) is a major vector of viral diseases like dengue fever, Zika and chikungunya. Aedes aegypti exhibits high morphological and behavioral variation, some of which is thought to be of epidemiological significance. Globally distributed domestic Ae. aegypti have often been grouped into (i) the very pale variety queenslandensis and (ii) the type form. Because the two color forms co-occur across most of their range, there is interest in understanding how freely they interbreed. This knowledge is particularly important for control strategies that rely on mating compatibilities between the release and target mosquitoes, such as Wolbachia releases and SIT. To address this question, we analyzed nuclear and mitochondrial genome-wide variation in the co-occurring pale and type Ae. aegypti from northern Queensland (Australia) and Singapore.Methods/FindingsWe typed 74 individuals at a 1170 bp-long mitochondrial sequence and at 16,569 nuclear SNPs using a customized double-digest RAD sequencing. 11/29 genotyped individuals from Singapore and 11/45 from Queensland were identified as var. queenslandensis based on the diagnostic scaling patterns. We found 24 different mitochondrial haplotypes, seven of which were shared between the two forms. Multivariate genetic clustering based on nuclear SNPs corresponded to individuals’ geographic location, not their color. Several family groups consisted of both forms and three queenslandensis individuals were Wolbachia infected, indicating previous breeding with the type form which has been used to introduce Wolbachia into Ae. aegypti populations.ConclusionAedes aegypti queenslandensis are genomically indistinguishable from the type form, which points to these forms freely interbreeding at least in Australia and Singapore. Based on our findings, it is unlikely that the presence of very pale Ae. aegypti will affect the success of Aedes control programs based on Wolbachia-infected, sterile or RIDL mosquitoes.

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An elusive endosymbiont: Does Wolbachia occur naturally in Aedes aegypti?

Ross

Callahan

Yang

et al. 2020

Ecology and Evolution

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Wolbachia are maternally inherited endosymbiotic bacteria found within many insect species. Aedes mosquitoes experimentally infected with Wolbachia are being released into the field for Aedes‐borne disease control. These Wolbachia infections induce cytoplasmic incompatibility which is used to suppress populations through incompatible matings or replace populations through the reproductive advantage provided by this mechanism. However, the presence of naturally occurring Wolbachia in target populations could interfere with both population replacement and suppression programs depending on the compatibility patterns between strains. Aedes aegypti were thought to not harbor Wolbachia naturally but several recent studies have detected Wolbachia in natural populations of this mosquito. We therefore review the evidence for natural Wolbachia infections in A. aegypti to date and discuss limitations of these studies. We draw on research from other mosquito species to outline the potential implications of natural Wolbachia infections in A. aegypti for disease control. To validate previous reports, we obtained a laboratory population of A. aegypti from New Mexico, USA, that harbors a natural Wolbachia infection, and we conducted field surveys in Kuala Lumpur, Malaysia, where a natural Wolbachia infection has also been reported. However, we were unable to detect Wolbachia in both the laboratory and field populations. Because the presence of naturally occurring Wolbachia in A. aegypti could have profound implications for Wolbachia‐based disease control programs, it is important to continue to accurately assess the Wolbachia status of target Aedes populations.

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Fitness of wAlbB Wolbachia Infection in Aedes aegypti: Parameter Estimates in an Outcrossed Background and Potential for Population Invasion

Cited by 109 publications

References 36 publications

Wolbachia Infections in Aedes aegypti Differ Markedly in Their Response to Cyclical Heat Stress

Wolbachia Infections in Aedes aegypti Differ Markedly in Their Response to Cyclical Heat Stress

The queenslandensis and the type Form of the Dengue Fever Mosquito (Aedes aegypti L.) Are Genomically Indistinguishable

An elusive endosymbiont: Does Wolbachia occur naturally in Aedes aegypti?

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