Fitness comparison of <i>Plutella xylostella</i> on original and marginal hosts using age‐stage, two‐sex life tables

Yang, Fei-Ying; Chen, Junhui; Ruan, Qian-Qian; Wang, Beibei; Jiao, Lu; Qiao, Qing-Xuan; He, Weiyi; You, Minsheng

doi:10.1002/ece3.7804

Cited by 4 publications

(1 citation statement)

References 39 publications

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“…The diamondback moth ( P. xylostella ) is an important pest that preferentially infests different cultivated vegetables and wild cruciferous plants [ 29 , 30 ]. Nonetheless, it has been observed that P. xylostella is able to establish a stable population on the noncruciferous vegetable pea ( Pisum sativum L.) when the preferred host is absent [ 31 ]. Evidence has been provided that the DNA methylation of P. xylostella upon host transfer from Raphanus sativus L. to Arabidopsis thaliana L. undergoes reprogramming, which might influence adaptation-associated gene expression [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

Functional Characterization of Two RNA Methyltransferase Genes METTL3 and METTL14 Uncovers the Roles of m6A in Mediating Adaptation of Plutella xylostella to Host Plants

Wang

Lai

et al. 2022

IJMS

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N6-methyladenosine (m6A) is one of the major epigenetic modifications in eukaryotes. Although increasing functions of m6A have been identified in insects, its role in Plutella xylostella L. for host plant adaptation remains unclear. In the current study, we show that the m6A content of P. xylostella was relatively low in different developmental stages and tissues, with no significant differences. Two RNA methyltransferase genes, PxMETTL3 (methyltransferase-like 3) and PxMETTL14 (methyltransferase-like 14), were identified and characterized. PxMETTL3 could be transcribed into two transcripts, and PxMETTL14 had only one transcript; both of these genes were highly expressed in egg and adult stages and reproductive tissues. The CRISPR/Cas9-mediated knockout of PxMETTL3 (ΔPxMETTL3-2) or PxMETTL14 (ΔPxMETTL14-14) confirmed their function in m6A installation into RNA. Furthermore, upon transfer from an artificial diet to the host plant, the mutant strains were affected in terms of larval and pupal weight or adult emergence rate, while the wildtype (WT) strain did not exhibit any difference. In addition, the fecundity and egg hatching rate of the WT strain decreased significantly, whereas only the ΔPxMETTL14-14 mutant strain displayed significantly decreased fecundity. There seemed to be a tradeoff between the stress adaptation and reproduction in P. xylostella mediated by m6A modification. During host transfer, the expression of PxMETTL14 was consistent with the change in m6A content, which implied that PxMETTL14 could respond to host plant defense effectively, and may regulate m6A content. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the differentially expressed transcripts with changes in m6A levels revealed that the potential functions of m6A-related genes may be involved in steroid biosynthesis for larval performance and metabolic pathways for adult reproduction. Overall, our work reveals an epigenetic regulation mechanism for the rapid adaptation of P. xylostella to variations in the host environment.

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Section: Introductionmentioning

confidence: 99%

Functional Characterization of Two RNA Methyltransferase Genes METTL3 and METTL14 Uncovers the Roles of m6A in Mediating Adaptation of Plutella xylostella to Host Plants

Wang

Lai

et al. 2022

IJMS

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Transgenerational Effect of Different Collard Green Genotypes On Plutella xylostella (Lepidoptera: Plutellidae)

Marques Pinheiro,

Lopes Baldin,

Fernandes Canassa

et al. 2024

Journal of Crop Health

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Landscape simplification increases the risk of infestation by the polyphagous pest Helicoverpa armigera for walnut, a novel marginal host

et al. 2022

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Fitness comparison of Plutella xylostella on original and marginal hosts using age‐stage, two‐sex life tables

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 4 publications

References 39 publications

Functional Characterization of Two RNA Methyltransferase Genes METTL3 and METTL14 Uncovers the Roles of m6A in Mediating Adaptation of Plutella xylostella to Host Plants

Functional Characterization of Two RNA Methyltransferase Genes METTL3 and METTL14 Uncovers the Roles of m6A in Mediating Adaptation of Plutella xylostella to Host Plants

Transgenerational Effect of Different Collard Green Genotypes On Plutella xylostella (Lepidoptera: Plutellidae)

Landscape simplification increases the risk of infestation by the polyphagous pest Helicoverpa armigera for walnut, a novel marginal host

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