Fitness and proteome changes accompanying the development of erythromycin resistance in a population of <i><scp>E</scp>scherichia coli</i> grown in continuous culture

Petráčková, Denisa; Janeček, J.; Bezoušková, Silvia; Kalachová, Ľubica; Techniková, Zuzana; Buriánková, Karolína; Halada, Petr; Haladová, Kateřina; Weiser, Jaroslav

doi:10.1002/mbo3.121

Cited by 7 publications

(7 citation statements)

References 27 publications

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“…Response to stress by bacteriostatic antibiotics acting on the ribosome (as macrolides, chloramphenicol, or tetracyclines) is likely to be manifest by the reduction in protein synthesis, which might reduce the building-up of (p)ppGpp, but that might be overcompensated by reduced degradation of this nucleotide (43,44). Proteome analysis of erythromycin-exposed "permeable" E. coli suggests a RpoS-regulated profile (45). In fact, sub-inhibitory concentrations of bacteriostatic antibiotics induce the stringent response, leading to beta-lactam tolerance (46).…”

Section: Discussionmentioning

confidence: 99%

Antibiotic killing of diversely generated populations of non-replicating bacteria

Shah

McCall

Govindan

et al. 2018

Preprint

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Non-replicating bacteria are known to be (or at least commonly thought to be) refractory to antibiotics to which they are genetically susceptible. Here, we explore the sensitivity to killing by bactericidal antibiotics of three classes of non-replicating populations of planktonic bacteria; (1) stationary phase, when the concentration of resources and/or nutrients are too low to allow for population growth; (2) persisters, minority subpopulations of susceptible bacteria surviving exposure to bactericidal antibiotics; (3) antibiotic-static cells, bacteria exposed to antibiotics that prevent their replication but kill them slowly if at all, the so-called bacteriostatic drugs. Using experimental populations of Staphylococcus aureus Newman and Escherichia coli K12 (MG1655) and respectively 9 and 7 different bactericidal antibiotics, we estimate the rates at which these drugs kill these different types of non-replicating bacteria. Contrary to the common belief that bacteria that are non-replicating are refractory to antibiotic-mediated killing, all three types of non-replicating populations of these Gram-positive and Gram-negative bacteria are consistently killed by aminoglycosides and the peptide antibiotics, daptomycin and colistin, respectively. This result indicates that non-replicating cells, irrespectively of why they do not replicate, have an almost identical response to bactericidal antibiotics. We discuss the implications of these results to our understanding of the mechanisms of action of antibiotics and the possibility of adding a short-course of aminoglycosides or peptide antibiotics to conventional therapy of bacterial infections.

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Section: Discussionmentioning

confidence: 99%

Antibiotic killing of diversely generated populations of non-replicating bacteria

Shah

McCall

Govindan

et al. 2018

Preprint

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“…This suggests that reducing the use of antibiotics will not necessarily reduce resistant pathogens in the population. The maintenance of resistance phenotypes in bacteria could also be influenced by the fitness cost associated with resistance (48) and the likelihood of developing compensatory fitness-improving mutations (49). These can potentially make it difficult to control antibiotic resistance in pathogenic E. coli and, more generally, in pathogenic infections.…”

Section: Discussionmentioning

confidence: 99%

Effects of Selection Pressure and Genetic Association on the Relationship between Antibiotic Resistance and Virulence in Escherichia coli

Zhang

Lévy

Trueba

et al. 2015

Antimicrob Agents Chemother

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e Antibiotic selection pressure and genetic associations may lead to the cooccurrence of resistance and virulence in individual pathogens. However, there is a lack of rigorous epidemiological evidence that demonstrates the cooccurrence of resistance and virulence at the population level. Using samples from a population-based case-control study in 25 villages in rural Ecuador, we characterized resistance to 12 antibiotics among pathogenic (n ‫؍‬ 86) and commensal (n ‫؍‬ 761) Escherichia coli isolates, classified by the presence or absence of known diarrheagenic virulence factor genes. The prevalences of resistance to single and multiple antibiotics were significantly higher for pathogenic isolates than for commensal isolates. Using a generalized estimating equation, antibiotic resistance was independently associated with virulence factor carriage, case status, and antibiotic use (for these respective factors: odds ratio [OR] ‫؍‬ 3.0, with a 95% confidence interval [CI] of 1.7 to 5.1; OR ‫؍‬ 2.0, with a 95% CI of 1.3 to 3.0; and OR ‫؍‬ 1.5, with a 95% CI of 0.9 to 2.5). Virulence factor carriage was more strongly related to antibiotic resistance than antibiotic use for all antibiotics examined, with the exception of fluoroquinolones, gentamicin, and cefotaxime. This study provides epidemiological evidence that antibiotic resistance and virulence factor carriage are linked in E. coli populations in a community setting. Further, these data suggest that while the cooccurrence of resistance and virulence in E. coli is partially due to antibiotic selection pressure, it is also genetically determined. These findings should be considered in developing strategies for treating infections and controlling for antibiotic resistance.A ntimicrobial resistance is a major public health problem, identified by the World Health Organization (WHO) as one of the most pressing issues in global health (1). In the United States, more than 2 million people per year acquire bacterial infections that are resistant to antibiotics, resulting in at least 23,000 deaths and expenditures of 20 to 35 billion dollars annually (2). Elsewhere, losses to the gross domestic product (GDP) from antibiotic resistance are estimated to be 0.4% to 1.6% (3). Antibiotic resistance can occur in any bacteria but is of direct concern among pathogens, because of the resulting increased duration of care, cost of treatment, morbidity, and mortality (4, 5). Although selection due to antibiotic use is considered a major factor associated with increased antibiotic resistance among pathogens, studies on the molecular mechanisms of resistance and virulence suggest a genetic association. For example, genes for both resistance and virulence can be colocalized in the genome, indicating coselection (6). While these mostly laboratory-based studies suggest a plausible ecological and biological link, there is a lack of rigorous epidemiological evidence demonstrating this link between antibiotic resistance and virulence in human populations.Pathogenic Escherichia coli and shigel...

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“…Proteome analysis of erythromycin-exposed “permeable” E. coli suggests a RpoS-regulated profile (45). In fact, subinhibitory concentrations of bacteriostatic antibiotics induce the stringent response, leading to beta-lactam tolerance (46).…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Killing of Diversely Generated Populations of Nonreplicating Bacteria

McCall

Shah

Govindan

et al. 2019

Antimicrob Agents Chemother

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Nonreplicating bacteria are known to be (or at least commonly thought to be) refractory to antibiotics to which they are genetically susceptible. Here, we explore the sensitivity to killing by bactericidal antibiotics of three classes of nonreplicating populations of planktonic bacteria: (i) stationary phase, when the concentration of resources and/or nutrients are too low to allow for population growth; (ii) persisters, minority subpopulations of susceptible bacteria surviving exposure to bactericidal antibiotics; and (iii) antibiotic-static cells, bacteria exposed to antibiotics that prevent their replication but kill them slowly if at all, the so-called bacteriostatic drugs. Using experimental populations of Staphylococcus aureus Newman and Escherichia coli K-12 (MG1655) and, respectively, nine and seven different bactericidal antibiotics, we estimated the rates at which these drugs kill these different types of nonreplicating bacteria. In contrast to the common belief that bacteria that are nonreplicating are refractory to antibiotic-mediated killing, all three types of nonreplicating populations of these Gram-positive and Gram-negative bacteria are consistently killed by aminoglycosides and the peptide antibiotics daptomycin and colistin, respectively. This result indicates that nonreplicating cells, irrespectively of why they do not replicate, have an almost identical response to bactericidal antibiotics. We discuss the implications of these results to our understanding of the mechanisms of action of antibiotics and the possibility of adding a short-course of aminoglycosides or peptide antibiotics to conventional therapy of bacterial infections.

show abstract

Fitness and proteome changes accompanying the development of erythromycin resistance in a population of Escherichia coli grown in continuous culture

Cited by 7 publications

References 27 publications

Antibiotic killing of diversely generated populations of non-replicating bacteria

Antibiotic killing of diversely generated populations of non-replicating bacteria

Effects of Selection Pressure and Genetic Association on the Relationship between Antibiotic Resistance and Virulence in Escherichia coli

Antibiotic Killing of Diversely Generated Populations of Nonreplicating Bacteria

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