FITC–quencher based caspase 3-activatable nanoprobes for effectively sensing caspase 3 in vitro and in cells

Tang, Aihua; Mei, Bin; Wang, Weijuan; Hu, Wanglai; Li, Fang; Zhou, Jun; Yang, Qing; Cui, Hua; Wu, Mian; Liang, Gaolin

doi:10.1039/c3nr03339b

Cited by 26 publications

(26 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…8,9 And among MMPs, MMP-2 plays a dominant role in tumor invasion and metastasis, which can be used for cancer diagnosis and tumor tissue specific therapeutic target. [10][11][12] Besides, 30 intracellular caspase-3 is the biomarker for early stage apoptotic cells, 13 and its activity can reflect real-time apoptosis information 14,15 which has great implications in therapy efficiency evaluation and apoptosis related drugs screening. [16][17][18] To date, various optical probes have been designed for MMPs 2,3,19 or 35 caspases imaging.…”

Section: Screening and Dynamic Apoptosis Imagingmentioning

confidence: 99%

A dual-FRET-based fluorescence probe for the sequential detection of MMP-2 and caspase-3

Li,

Liu,

Cheng

et al. 2015

Chem. Commun.

View full text Add to dashboard Cite

show abstract

Section: Screening and Dynamic Apoptosis Imagingmentioning

confidence: 99%

A dual-FRET-based fluorescence probe for the sequential detection of MMP-2 and caspase-3

Li,

Liu,

Cheng

et al. 2015

Chem. Commun.

View full text Add to dashboard Cite

show abstract

“…DEVD: Asp-Glu-Val-Asp; FITC: Fluorescein isothiocyanate. Adapted from [20] with permission from Springer. Nanoparticle-based caspase sensors Review physical properties that improve sensitivity, specificity and biocompatibility.…”

Section: Other Np-based Caspase Activity Sensorsmentioning

confidence: 98%

“…In some studies, GNP-based colorimetry and FRET were combined, providing a dual detection for caspase-3 activity (Figure 6) [20,23]. For example, the fluorescence of fluorescein isothiocyanate (FITC) conjugated to GNPs through a peptide containing a caspase-specific sequence is quenched when the nanoprobe is intact.…”

Section: Gnp-based Caspase Activity Sensorsmentioning

confidence: 99%

“…After caspase cleavage, FITC is future science group Nanoparticle-based caspase sensors Review released and an increase of its fluorescence is measured. At the same time, release of the negatively charged FITC-DEVD-OH structure from GNPs results in a decrease in their electrostatic stability, leading to their aggregation and a change of color from red to blue ( Figure 6) [20].…”

Section: Gnp-based Caspase Activity Sensorsmentioning

confidence: 99%

See 1 more Smart Citation

Nanoparticle-based caspase sensors

Maysinger

Hutter

2015

Nanomedicine

View full text Add to dashboard Cite

Recent advances in nanotechnology have provided new tools for measuring enzymatic activities that are relevant for the assessment of physiological and pathological processes. Caspases, the enzymes intimately linked with cell death and inflammation, are cysteine-dependent aspartate-directed proteases. The measurement of caspase activity requires assays that can provide data with specificity, precision and sensitivity. Several nanoparticle-based assays are now beginning to emerge. This article will first provide a brief discussion of conventional methods of measuring caspase activity and their limitations, followed by an overview of the advantages and limitations of nanoparticle-based strategies for sensing caspase enzymatic activity in vitro and in vivo.

show abstract

“…This highlights a major failing of some approaches to separating oil from water; removal of the oil from the material after separation has occurred. Recent reports also include the fabrication of water purification devices [22–24]. These are enclosed systems which use a flow of solvent/water mixtures through a superhydrophobic or superoleophobic filter.…”

Section: Introductionmentioning

confidence: 99%

Superhydrophobic silica wool—a facile route to separating oil and hydrophobic solvents from water

Crick

Bhachu

Parkin

2014

Science and Technology of Advanced Materials

View full text Add to dashboard Cite

Silica microfiber wool was systematically functionalized in order to provide an extremely water repellent and oleophilic material. This was carried out using a two-step functionalization that was shown to be a highly effective method for generating an intense water repulsion and attraction for oil. A demonstration of the silica wools application is shown through the highly efficient separation of oils and hydrophobic solvents from water. Water is confined to the extremities of the material, while oil is absorbed into the voids within the wool. The effect of surface functionalization is monitored though observing the interaction of the material with both oils and water, in addition to scanning electron microscope images, x-ray photoelectron spectroscopy and energy dispersive x-ray analysis. The material can be readily utilized in many applications, including the cleaning of oil spills and filtering during industrial processes, as well as further water purification tasks—while not suffering the losses of efficiency observed in current leading polymeric materials.

show abstract

FITC–quencher based caspase 3-activatable nanoprobes for effectively sensing caspase 3 in vitro and in cells

Abstract: By employing fluorescence resonance energy transfer (FRET) quenching, we rationally designed two new FITC-quencher based nanoprobes for effectively sensing caspase 3 (Casp3) in vitro and in cells. Our nanoprobes hold promise for assessing the chemotherapeutic effect of cancer treatment.

Cited by 26 publications

References 48 publications

A dual-FRET-based fluorescence probe for the sequential detection of MMP-2 and caspase-3

A dual-FRET-based fluorescence probe for the sequential detection of MMP-2 and caspase-3

Nanoparticle-based caspase sensors

Superhydrophobic silica wool—a facile route to separating oil and hydrophobic solvents from water

Contact Info

Product

Resources

About