Fission yeast nucleolar protein Dnt1 regulates G2/M transition and cytokinesis by downregulating Wee1 kinase

Abstract

There was an error published in J. Cell Sci. 126, 4995-5004.One source of funding was accidentally omitted. The correct funding section is as shown below.

A haploid genetic screen identifies the G1/S regulatory machinery as a determinant of Wee1 inhibitor sensitivity

“…Furthermore, our results are in line with yeast studies, in which Wee1 has a function beyond mitotic entry. Experiments with S. pombe showed that Wee1 regulation is part of the septation initiation network (SIN) during mitotic exit (45). In addition, the S. cervisiae Wee1 ortholog Swe1 constitutes an anaphase checkpoint, which controls proper activation of the APC/C to allow mitotic exit (46).…”

Facile manipulation of protein localization in fission yeast through binding of GFP-binding protein to GFP

“…The coding sequence of firefly luciferase was PCR amplified using two sets of primers (#1305-1308, Table S3) and plasmid pFA6a-Firefly-luciferasekanMX6 (Yu et al, 2013) as template. Linear complementary backbone of pJK148-P nmt -GBP-mCherry(N) (where P nmt is P nmt81 , P nmt41 or P nmt1 ) or pUC119-P adh -GBP-mCherry(N)-hphMX6-lys1* (where P adh is P adh81 , P adh21 , P adh11 or P adh1 ) was also produced in two parallel PCRs using two sets of non-tailed or tailed primers (#1206, #1207, #1210, #1211, and #1321-1324; Table S3).…”

“…In order to construct genomically tagged Alp4-R.luciferase, which can be used as an internal control for luciferase activity measurement, pFA6a-Renilla-luciferase-natMX6 (Yu et al, 2013) was used to add a C-terminal Renilla luciferase tag to the endogenous alp4 + locus to create the Alp4-R. luciferase (nat R ) allele using PCR-based gene targeting (Bahler et al, 1998).…”

“…Yeast cell extracts were prepared in lysis buffer (120 mM Tris-HCl, pH 6.8, 4% SDS, 20% glycerol, 8 M urea, 0.6 M β-mercaptoethanol) using glass bead disruption and a FastPrep homogenizer (MP Biomedical), as described previously (Yu et al, 2013). For detection after PAGE electrophoresis, rabbit polyclonal anti-mCherry antibody (ab167453, Abcam) (Morozova et al, 2016) was used as the primary antibody (1:1000 dilution), and Cdc2 was detected using rabbit polyclonal anti-PSTAIRE (sc-53, Santa Cruz Biotechnology) (Yu et al, 2013) as a loading control (1:1000 dilution).…”

“…For detection after PAGE electrophoresis, rabbit polyclonal anti-mCherry antibody (ab167453, Abcam) (Morozova et al, 2016) was used as the primary antibody (1:1000 dilution), and Cdc2 was detected using rabbit polyclonal anti-PSTAIRE (sc-53, Santa Cruz Biotechnology) (Yu et al, 2013) as a loading control (1:1000 dilution). Goat anti-rabbit-IgG conjugated to horseradish peroxidase (Pierce) was used as the secondary antibody at 1:10,000 dilution.…”