Fish viperin exerts a conserved antiviral function through RLR-triggered IFN signaling pathway

Wang, Bing; Zhang, Yibing; Liu, Ting-Kai; Shi, Jing; Sun, Fei; Gui, Jian‐Fang

doi:10.1016/j.dci.2014.07.006

Cited by 60 publications

(32 citation statements)

References 59 publications

(86 reference statements)

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“…As in mammals, fish type I IFNs are able to induce expression of a variety of antiviral genes, including myxovirus resistance (Mx), protein kinase R (PKR), virus inhibitory protein, endoplasmic reticulum-associated, IFN-inducible (Viperin), and IFN-stimulated gene (ISG) 15, thus leading to an enhanced antiviral state (4, 10–12). Accumulating data suggest that fish group I and II type I IFNs may have distinct antiviral roles in different cells or at different stages of infection (3).…”

Section: Introductionmentioning

confidence: 99%

Identification of Two Subgroups of Type I IFNs in Perciforme Fish Large Yellow Croaker Larimichthys crocea Provides Novel Insights into Function and Regulation of Fish Type I IFNs

Ding

Huang

et al. 2016

Front. Immunol.

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Like mammals, fish possess an interferon regulatory factor (IRF) 3/IRF7-dependent type I IFN responses, but the exact mechanism by which IRF3/IRF7 regulate the type I IFNs remains largely unknown. In this study, we identified two type I IFNs in the Perciforme fish large yellow croaker Larimichthys crocea, one of which belongs to the fish IFNd subgroup and the other is assigned to a novel subgroup of group I IFNs in fish, tentatively termed IFNh. The two IFN genes are constitutively expressed in all examined tissues, but with varied expression levels. Both IFN genes can be rapidly induced in head kidney and spleen tissues by polyinosinic–polycytidylic acid. The recombinant IFNh was shown to be more potent to trigger a rapid induction of the antiviral genes MxA and protein kinase R than the IFNd, suggesting that they may play distinct roles in regulating early antiviral immunity. Strikingly, IFNd, but not IFNh, could induce the gene expression of itself and IFNh through a positive feedback loop mediated by the IFNd-dependent activation of IRF3 and IRF7. Furthermore, our data demonstrate that the induction of IFNd can be enhanced by the dimeric formation of IRF3 and IRF7, while the IFNh expression mainly involves IRF3. Taken together, our findings demonstrate that the IFN responses are diverse in fish and are likely to be regulated by distinct mechanisms.

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Section: Introductionmentioning

confidence: 99%

Identification of Two Subgroups of Type I IFNs in Perciforme Fish Large Yellow Croaker Larimichthys crocea Provides Novel Insights into Function and Regulation of Fish Type I IFNs

Ding

Huang

et al. 2016

Front. Immunol.

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“…The type I IFNs bind to specific cell surface receptors to induce the JAK-STAT signaling pathway that results in the expression of a set of antiviral proteins . Many molecules (MAVS,IRF1 and viperin) have been proven to exert conserved functions in fish (Feng et al, 2015;Wang et al, 2014;Zhang et al, 2014). Additionally, identification of the novel fish-specific antiviral genes (Gig1, Gig2 and DreI) has shown that fish type I IFNs can function in a unique way (Li et al, 2012;Sun et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

The kinetics and protection of the antiviral state induced by recombinant iIFN1a in rainbow trout against infectious hematopoietic necrosis virus

Cao

LaPatra

et al. 2016

Molecular Immunology

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“…Overexpression of Mxs blocks the replication of GCRV and delays the CPE induced by GCRV infection [10]. In crucian carp, viperin confers cells significant protection against GCRV infection [11].…”

Section: Introductionmentioning

confidence: 99%

Grass carp reovirus VP56 represses interferon production by degrading phosphorylated IRF7

Zhang

et al. 2020

Fish & Shellfish Immunology

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Grass carp reovirus (GCRV) is an efficient pathogen causing high mortality in grass carp, meanwhile, fish interferon (IFN) is a powerful cytokine enabling host cells to establish an antiviral state; therefore, the strategies used by GCRV to escape the cellular IFN response need to be investigated. Here, we report that GCRV VP56 inhibits host IFN production by degrading the transcription factor IFN regulatory factor 7 (IRF7). First, overexpression of VP56 inhibited the IFN production induced by the polyinosinic-polycytidylic acid (poly I:C) and mitochondrial antiviral signaling protein (MAVS), while the capacity of IRF7 on IFN induction was unaffected. Second, VP56 interacted with RLRs but did not affect the stabilization of the proteins in the normal state, while the phosphorylated IRF7 activated by TBK1 was degraded by VP56 through K48-linked ubiquitination. Finally, overexpression of VP56 remarkably reduced the host cellular ifn transcription and facilitated viral proliferation. Taken together, our results demonstrate that GCRV VP56 suppresses the host IFN response by targeting phosphorylated IRF7 for ubiquitination and degradation.

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Fish viperin exerts a conserved antiviral function through RLR-triggered IFN signaling pathway

Cited by 60 publications

References 59 publications

Identification of Two Subgroups of Type I IFNs in Perciforme Fish Large Yellow Croaker Larimichthys crocea Provides Novel Insights into Function and Regulation of Fish Type I IFNs

Identification of Two Subgroups of Type I IFNs in Perciforme Fish Large Yellow Croaker Larimichthys crocea Provides Novel Insights into Function and Regulation of Fish Type I IFNs

The kinetics and protection of the antiviral state induced by recombinant iIFN1a in rainbow trout against infectious hematopoietic necrosis virus

Grass carp reovirus VP56 represses interferon production by degrading phosphorylated IRF7

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