Fish proteome analysis: Model organisms and non‐sequenced species

Abstract

In the last decade, proteomic technologies have been increasingly used in fish biology research. Proteomics has been applied primarily to investigate the physiology, development biology and the impact of contaminants in fish model organisms, such as zebrafish (Danio rerio), as well as in some commercial species produced in aquaculture, mainly salmonids and cyprinids. However, the lack of previous genetic information on most fish species has been a major drawback for a more general application of the different proteomic technologies currently available. Also, many teleosts of interest in biological research and with potential application in aquaculture hold unique physiological characteristics that cannot be directly addressed from the study of small laboratory fish models. This review describes proteomic approaches that have been used to investigate diverse biological questions in model and nonmodel fish species. We will also evaluate the current possibilities to integrate fish proteomics with other ''omic'' approaches, as well as with additional complementary techniques, in order to address the future challenges in fish biology research.

Toxicogenomic applications of Chinese rare minnow (Gobiocypris rarus) in aquatic toxicology

“…Fish species comprise a major percentage within the vertebrate group, with a distribution that covers practically all aquatic ecosystems (Forné et al, 2010). Teleosts represent more than half of all vertebrate species and are adapted to a wide range of marine and freshwater habitats (Nelson, 1994).…”

Analysis of proteomic profile changes of zebrafish embryos during exposure to doxorubicin, built-in the phospholipid transport nanosystem

“…Для анализа белков эмбрионов D. rerio традиционно используют протеомную технологию, основанную на разделении сложной белковой смеси с помощью двумерного электрофореза и дальнейшей масс-спектрометрии [1]. Однако разделение белков с использованием одномерного электрофореза не потеряло своей актуальности для быстрого выявления изменений протеома [13,14].…”

“…Рыбы вида Danio rerio (зебрафиш) в последние годы стали использовать как эффективный модельный организм на ранних этапах разработки лекарственных препаратов для обнаружения их эффективности и токсичности [1]. Основными преимуществами эмбрионов D. rerio является то, что они легко проницаемы для небольших молекул, добавленных непосредственно в инкубационную среду, развиваются быстро и проходят стадии от яйца до личинки всего за 3 дня.…”

Advances in genomics for flatfish aquaculture

“…During the last years, an important effort has been directed towards the use of functional genomics, proteomics and metabolomics to better characterize reproduction, development, nutrition, immunity and toxicology of flatfishes (reviewed by Cerdà et al 2008Cerdà et al , 2010Forné et al 2010). Such comprehensive functional analyses aim at identifying the critical genes and molecules that control these physiological processes in order to improve current flatfish farming technologies.…”