Fish oil preparation inhibits leukocyte recruitment and bands that characterize inflamed tissue in a model of phenol-induced skin inflammation: percutaneous penetration of a topically applied preparation demonstrated by photoacoustic spectroscopy

Abstract

Fish oil (FO) is a natural source of omega-3 fatty acids, with well-established beneficial effects in inflammatory diseases when FO is orally administered. This study investigated the effects of a topically applied FO preparation (FOP) on phenol-induced ear edema and evaluated the percutaneous penetration of FOP in ear tissue. After applying phenol, groups of mice received an application of FOP on the ear. After 1 h, ear tissue was collected to determine the percent inhibition of edema, determine myeloperoxidase activity, and perform photoacoustic spectroscopy (PAS). Treatment with FOP did not reduce edema, but reduced myeloperoxidase activity. The FOP decreased the area of bands that characterize inflamed tissue and penetrated into the tissue. These results indicated an inhibitory effect of FOP on leukocyte recruitment in phenol-induced ear edema. These data support the applicability of PAS as a non-destructive method for evaluating the inflammatory response, percutaneous penetration and antiinflammatory activity of compounds.

Baccharis dracunculifolia DC (Asteraceae) Root Extract and Its Triterpene Baccharis Oxide Display Topical Anti‐Inflammatory Effects on Different Mice Ear Edema Models

“…Moreover, in the phenol-induced edema model, the direct contact of phenol with the skin may cause rupture of the keratinocyte membranes, resulting in releasing of many cytokines, such as IL-8, IL-1 α , and TNF- α , which leads to the release of other inflammatory mediators, like AA metabolites and ROS [ 39 ]. Then, phenol is considered a suitable inflammatory agent for simulating dermatitis in mice [ 40 , 41 ]. In this model, both BdR and BOx demonstrated significant reduction in ear edema, with noticeable potential in the treatment of contact dermatitis, suggesting that their anti-inflammatory activities may also be related to a possible decrease in the production of AA metabolites and/or with some antioxidant likely properties, which should be further explored in the future.…”

Fish oil as effective supplement preventing inflammatory and histopathological alterations in adjuvant-induced arthritis in rats

“…The FOP consisted of fish oil diluted in olive oil, which was used to enable the administration of FO in very low amounts (35 and 75 mg/kg). The choice of olive oil (Oo) to dilute the FO was based on our previous studies that evaluated the beneficial effects of FO on the acute inflammatory response and recruitment of leukocytes into the joint cavity in arthritic rats (Ames et al, 2017;Ames et al, 2020;Arruda et al, 2017;Estevão-Silva et al, 2016).…”

Development status of novel spectral imaging techniques and application to traditional Chinese medicine

“…Preliminary progress has been achieved in the photoacoustic imaging technology research for evaluating drug pharmacodynamics, particularly those of TCM extracts. This technology was used by Ames et al [ 37 ] to assess the anti-inflammatory and percutaneous osmotic effects of fish oil. Mendes et al.…”