Fish Oil - Omega-3 Exerts Protective Effect in Oxidative Stress and Liver Dysfunctions Resulting from Experimental Sepsis

Velasque, Mary Johnson Soares Gonçalves; Branchini, Gisele; Catarina, Anderson Velasque; Bettoni, Lais; Fernandes, Rodrigo; Silva, Arthur Ferreira da; Dorneles, Gilson Pires; Silva, Igor Martins da; Santos, Maeli Andressa; Sumienski, Juliana; Peres, Alessandra; Roehe, Adriana Vial; Kohek, Maria Beatriz da Fonte; Porawski, Marilene; Nunes, Fernanda Bordignon

doi:10.1016/j.jceh.2022.07.001

Cited by 6 publications

(4 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…If hepatotoxicity is present, an elevated ALT is expected. This further substantiates the claims that Omega-3 is an antioxidant and reduces oxidative stress (Katrenčíková et al, 2021, Velasque et al, 2023.…”

Section: Discussionsupporting

confidence: 83%

Protective Effects of Omega-3 Fatty Acids Against Cisplatin Induced Hepatocellular Damage and Oxidative Stress in Rats

ALRAMADNEH,

Abu-Harirah,

Saleh

et al. 2023

Preprint

View full text Add to dashboard Cite

Cisplatin has a high rate of effectiveness in treating cancer, its use is restricted due to the severe adverse consequences it produces. Nephrotoxicity, neurotoxicity, and ototoxicity are only a few of the unwanted side effects. In the case of cisplatin, hepatotoxicity is thought to be caused by oxidative stress brought on by an increase in reactive oxygen species (ROS). Omega-3 is an antioxidant that reduces the production of reactive oxygen species by inhibiting the enzyme nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. This study aimed to compare the beneficial effects of omega-3 therapy against the harmful effects of cisplatin-induced hepatotoxicity and to determine the effect of cisplatin on hepatic pro-oxidant or antioxidant systems. The rats were divided into four groups (n = 6 per group) and treated with single dosages of cisplatin (5 milligrams per kilogram) and omega-3 (20 milligrams per kilogram over fourteen days by intraperitoneal administration), or cisplatin combined with omega-3. In addition, the rat’s blood was tested for alkaline phosphatase, aspartate transaminase, and alanine transaminase levels. We observed higher levels of lipid peroxidation products, total oxidant status, and ALT in the cisplatin group compared to the control group. Conversely, it was revealed that glutathione peroxidase and superoxide dismutase levels were considerably lower in the cisplatin group compared to the control group. Omega-3 has significantly reduced the toxicity of cisplatin in the liver. Our biochemical results confirmed our histological observations of central venous dilations, pericenter and periportal sinusoidal dilations, parenchymal inflammation, vacuolar abnormalities in hepatocytes, biliary duct proliferation, and caspase-3 positive hepatocytes. In conclusion, Omega-3 can provide biochemical and histological protection against cisplatin-induced hepatotoxicity.

show abstract

Section: Discussionsupporting

confidence: 83%

Protective Effects of Omega-3 Fatty Acids Against Cisplatin Induced Hepatocellular Damage and Oxidative Stress in Rats

ALRAMADNEH,

Abu-Harirah,

Saleh

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…It is important to highlight that α-tocopherol, a form of vitamin E, plays a crucial role in protecting the integrity of the cell membrane against oxidative damage in collaboration with other antioxidants, such as vitamin C and GPx. The present study indicates that antioxidant activities were lower in supplemented animals, which can be attributed to the use of these antioxidants to contain the development of hepatic steatosis since omega-3, by itself, exerts a protective effect against free radicals, reducing the need for high levels of antioxidants [ 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Dietary Supplementation with Omega-3 Fatty Acid on the Generation of Regulatory T Lymphocytes and on Antioxidant Parameters and Markers of Oxidative Stress in the Liver Tissue of IL−10 Knockout Mice

Campanari,

Cipriano,

Fraga-Silva

et al. 2024

Nutrients

View full text Add to dashboard Cite

Introduction: chronic low-grade inflammation, or inflammaging, emerges as a crucial element in the aging process and is associated with cardiovascular and neurological diseases, sarcopenia, and malnutrition. Evidence suggests that omega-3 fatty acids present a potential therapeutic agent in the prevention and treatment of inflammatory diseases, mitigating oxidative stress, and improving muscle mass, attributes that are particularly relevant in the context of aging. The objective of the present study was to evaluate the effectiveness of supplementation with omega-3 fish oil in improving the immune response and oxidative stress in knockout mice for interleukin IL−10 (IL−10−/−). Material and methods: female C57BL/6 wild-type (WT) and interleukin IL−10 knockout (IL−10−/−) mice were fed during 90 days with a standard diet (control groups), or they were fed/supplemented with 10% of the omega-3 polyunsaturated fatty acid diet (omega-3 groups). Muscle, liver, intestinal, and mesenteric lymph node tissue were collected for analysis. Results: the IL−10−/−+O3 group showed greater weight gain compared to the WT+O3 (p = 0.001) group. The IL−10−/−+O3 group exhibited a higher frequency of regulatory T cells than the IL−10−/− group (p = 0.001). It was found that animals in the IL−10−/−+O3 group had lower levels of steatosis when compared to the IL−10−/− group (p = 0.017). There was even greater vitamin E activity in the WT group compared to the IL−10−/−+O3 group (p = 0.001) and WT+O3 compared to IL−10−/−+O3 (p = 0.002), and when analyzing the marker of oxidative stress, MDA, an increase in lipid peroxidation was found in the IL−10−/−+O3 group when compared to the IL−10−/− group (p = 0.03). Muscle tissue histology showed decreased muscle fibers in the IL−10−/−+O3, IL−10−/−, and WT+O3 groups. Conclusion: the findings show a decrease in inflammation, an increase in oxidative stress markers, and a decrease in antioxidant markers in the IL−10−/−+O3 group, suggesting that supplementation with omega-3 fish oil might be a potential intervention for inflammaging that characterizes the aging process and age-related diseases.

show abstract

“…The use of fish oil (rich in omega-3 fatty acids) has demonstrated a potential protective effect against organic dysfunction caused by sepsis, evidenced by the observed decrease in lactate levels in the serum of septic Wistar rats and mitigating liver injury by lowering ALT levels ( Velasque et al., 2023 ). Omega-3 fatty acid treatment has also led to a decrease in ROS and OS, resulting in reduced levels of TBARS and DCF (2’,7’-dichlorofluorescein, a fluorescent product for detecting ROS) in serum and liver tissue.…”

Section: Antioxidant Treatmentmentioning

confidence: 99%

“…Additionally, this group showed higher levels of thiols in serum compared to the sepsis group. Levels of creatinine and urea were decreased as a result of omega-3 fatty acids treatment ( Velasque et al., 2023 ). The encouraging outcomes demonstrate that omega-3 fatty acids, which function as an anti-inflammatory and potent antioxidant, are a potentially effective treatment for liver injuries and alterations caused by sepsis, particularly in OS conditions ( Table 2 and Figure 5 ) ( Velasque et al., 2023 ).…”

Section: Antioxidant Treatmentmentioning

confidence: 99%

Exploring the role of antioxidants in sepsis-associated oxidative stress: a comprehensive review

Sahoo,

Wong,

Patani

et al. 2024

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Sepsis is a potentially fatal condition characterized by organ dysfunction caused by an imbalanced immune response to infection. Although an increased inflammatory response significantly contributes to the pathogenesis of sepsis, several molecular mechanisms underlying the progression of sepsis are associated with increased cellular reactive oxygen species (ROS) generation and exhausted antioxidant pathways. This review article provides a comprehensive overview of the involvement of ROS in the pathophysiology of sepsis and the potential application of antioxidants with antimicrobial properties as an adjunct to primary therapies (fluid and antibiotic therapies) against sepsis. This article delves into the advantages and disadvantages associated with the utilization of antioxidants in the therapeutic approach to sepsis, which has been explored in a variety of animal models and clinical trials. While the application of antioxidants has been suggested as a potential therapy to suppress the immune response in cases where an intensified inflammatory reaction occurs, the use of multiple antioxidant agents can be beneficial as they can act additively or synergistically on different pathways, thereby enhancing the antioxidant defense. Furthermore, the utilization of immunoadjuvant therapy, specifically in septic patients displaying immunosuppressive tendencies, represents a promising advancement in sepsis therapy.

show abstract

Fish Oil - Omega-3 Exerts Protective Effect in Oxidative Stress and Liver Dysfunctions Resulting from Experimental Sepsis

Cited by 6 publications

References 63 publications

Protective Effects of Omega-3 Fatty Acids Against Cisplatin Induced Hepatocellular Damage and Oxidative Stress in Rats

Protective Effects of Omega-3 Fatty Acids Against Cisplatin Induced Hepatocellular Damage and Oxidative Stress in Rats

Effect of Dietary Supplementation with Omega-3 Fatty Acid on the Generation of Regulatory T Lymphocytes and on Antioxidant Parameters and Markers of Oxidative Stress in the Liver Tissue of IL−10 Knockout Mice

Exploring the role of antioxidants in sepsis-associated oxidative stress: a comprehensive review

Contact Info

Product

Resources

About