Fish oil administration mediates apoptosis of Walker 256 tumor cells by modulation of p53, Bcl-2, caspase-7 and caspase-3 protein expression

Borghetti, Gina; Yamaguchi, Adriana; Aikawa, Júlia; Yamazaki, Ricardo Key; Brito, Gleisson Alisson Pereira de; Fernandes, Luíz Cláudio

doi:10.1186/s12944-015-0098-y

Cited by 28 publications

(16 citation statements)

References 23 publications

(27 reference statements)

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“…Bel-7402 cells treated with Plac1 siRNA showed decreased expression of Bcl-2, which was inversely related to p53 expression, implying that downregulation of Bcl-2 expression in Bel-7402 cells might be suppressed by p53. This hypothesis is supported by previous studies showing that Bcl-2 is negatively regulated by the tumor suppressor p53 (23,24). Surprisingly, instead of upregulation, Bax expression was inhibited in Bel-7402 cells transfected with Plac1 siRNA.…”

Section: Discussionsupporting

confidence: 79%

Oncogenic function of Plac1 on the proliferation and metastasis in hepatocellular carcinoma cells

Lin

Xia

et al. 2016

Oncology Reports

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Placenta-specific protein 1 (Plac1), which is selectively expressed in the placental syncytiotrophoblast in adult normal tissues, plays an essential role in normal placental and embryonic development. Accumulating evidence suggests that enhanced Plac1 expression is closely associated with the progression of human cancer. Whether Plac1 contributes to the pathophysiology of hepatocellular carcinoma (HCC) remains unclear. In the present study, our data revealed that the expression of Plac1 in human HCC tissues was upregulated, which significantly correlated with metastasis of HCC. Knockdown of Plac1 by small interfering RNA (siRNA) in Bel-7402 and HepG2 cells resulted in decreasing tumor cell proliferation and increasing apoptosis, which implied the oncogenic potential of Plac1. Moreover, silencing of Plac1 induced G1 cell cycle arrest through suppression of cyclin D1 and CDK4 expression. Furthermore, depletion of Plac1 repressed epithelial-mesenchymal transition (EMT), with decreased cell migration and invasion, supporting upregulated E-cadherin expression and downregulated vimentin, twist and snail expression that characterize EMT. Further study suggested that decreased Plac1 expression attenuated the phosphorylation of Akt. These findings have uncovered that Plac1 plays a pivotal role in the progression of HCC, and may serve as a novel therapeutic target for HCC.

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Section: Discussionsupporting

confidence: 79%

Oncogenic function of Plac1 on the proliferation and metastasis in hepatocellular carcinoma cells

Lin

Xia

et al. 2016

Oncology Reports

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“…This translocation results in the release of cytochrome‐c into the cytosol, which forms a complex with factor activator of apoptosis 1 (Apaf‐1), activating caspases that subsequently trigger apoptosis . The caspase‐3 signalling mediating apoptosis in tumour cells has been described for several compounds, such as doxorubicin, eudesmol isomers , polyunsaturated fatty acids and polyacetylenes . For the first time, our data confirm the activation of caspase cascade by Syd‐1 in tumour cells.…”

Section: Discussionsupporting

confidence: 72%

Sydnone 1: A Mesoionic Compound with Antitumoral and Haematological Effects In Vivo

Galuppo

Lívero

Martins

et al. 2016

Basic Clin Pharma Tox

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This study evaluated the antitumour activity of the mesoionic compound sydnone 1 (Syd-1) against Walker-256 carcinosarcoma. Tumour cells were subcutaneously inoculated in the hind limb in male Wistar rats. The animals were orally treated for 12 days with Syd-1 (75 mg/kg) or vehicle. At the end of treatment, considerable decreases in tumour volume and tumour weight were observed in treated animals. Samples of these tumours presented increases in apoptotic bodies and pro-apoptotic protein expression (Bax and p53), while the expression of the anti-apoptotic protein Bcl-2 was reduced. A decrease in reduced glutathione levels and an increase in glutathione peroxidase activity were observed in tumour after Syd-1 treatment. However, significant splenomegaly was evident in animals that received Syd-1, most likely attributable to the induction of haemolysis. This study demonstrated the antitumour activity of Syd-1 against Walker-256 carcinosarcoma. Its mechanism of action is linked to the activation of apoptotic pathways that lead to tumour cell death.

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“…It will be interesting and important to conduct a lifetime tracking on the growth rate, health status, and life span of Fat-1 transgenic sheep, as well as other Fat-1 transgenic livestock, to provide important references for humans under the condition of high ALA or n-3 LC-PUFA intake. Several reports indicated that n-3 PUFAs had an anticancer effect by activating the p53 pathway [52][53][54][55]. However, in our case, the p53 signaling pathway was also down-regulated in transgenic-expressing cells.…”

Section: Discussioncontrasting

confidence: 60%

Comparative Transcriptome Analysis Provides Insights into the Polyunsaturated Fatty Acid Synthesis Regulation of Fat-1 Transgenic Sheep

Luo

Zheng

Yang

et al. 2020

IJMS

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Transgenic technology has huge application potential in agriculture and medical fields, such as producing new livestock varieties with new valuable features and xenotransplantation. However, how an exogenous gene affects the host animal’s gene regulation networks and their health status is still poorly understood. In the current study, Fat-1 transgenic sheep were generated, and the tissues from 100-day abnormal (DAF_1) and normal (DAF_2) fetuses, postnatal lambs (DAF_4), transgenic-silencing (DAFG5), and -expressing (DAFG6) skin cells were collected and subjected to transcriptome sequencing, and their gene expression profiles were compared in multiple dimensions. The results were as follows. For DAF_1, its abnormal development was caused by pathogen invasion but not the introduction of the Fat-1 gene. Fat-1 expression down-regulated the genes related to the cell cycle; the NF-κB signaling pathway and the PI3K/Akt signaling pathway were down-regulated, and the PUFAs (polyunsaturated fatty acids) biosynthesis pathway was shifted toward the biosynthesis of high-level n-3 LC-PUFAs (long-chain PUFAs). Four key node genes, FADS2, PPARA, PRKACA, and ACACA, were found to be responsible for the gene expression profile shift from the Fat-1 transgenic 100-day fetus to postnatal lamb, and FADS2 may play a key role in the accumulation of n-3 LC-PUFAs in Fat-1 transgenic sheep muscle. Our study provides new insights into the FUFAs synthesis regulation in Fat-1 transgenic animals.

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Fish oil administration mediates apoptosis of Walker 256 tumor cells by modulation of p53, Bcl-2, caspase-7 and caspase-3 protein expression

Cited by 28 publications

References 23 publications

Oncogenic function of Plac1 on the proliferation and metastasis in hepatocellular carcinoma cells

Oncogenic function of Plac1 on the proliferation and metastasis in hepatocellular carcinoma cells

Sydnone 1: A Mesoionic Compound with Antitumoral and Haematological Effects In Vivo

Comparative Transcriptome Analysis Provides Insights into the Polyunsaturated Fatty Acid Synthesis Regulation of Fat-1 Transgenic Sheep

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