FISH mapping of the porcine NPY5 gene to Chromosome 8p11

Törnsten, Anna; Wraith, Amanda; Larhammar, Dan; Chowdhary, Bhanu P.

doi:10.1007/s003359900742

Cited by 2 publications

(5 citation statements)

References 9 publications

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“…2). This localization is the same as our recent FISH assignment of the NPY5R gene (Tornsten et al 1998). However, the NPY2R BAC clone mapped on the other side of the centromere to the proximal end of SSC8q21 (Fig.…”

Section: Localization Of Npy Receptorssupporting

confidence: 60%

“…The NPY5R-containing phage clone had been isolated and mapped previously (Tornsten et al 1998). Coding regions were sequenced for each of the porcine genes and the predicted proteins were used in sequence alignments with the other two species for which all five sequences and mapping data are available, namely, human and mouse.…”

Section: Cloning and Sequence Analysis Of Porcine Npy Receptor Genesmentioning

confidence: 99%

“…The NPY1R and NPY4R-containing phage and the NPY2R BAC were physically assigned by FISH mapping on metaphase spreads according to Tornsten et al (1998). The distance between the NPY1R and NPY5R-containing phage clones was estimated with fiber-FISH as described elsewhere (Sjoberg et al 1997), in which signal detection, chromosome identification, imaging, and other details have also been described.…”

Section: Fish Mappingmentioning

confidence: 99%

“…A genomic phage (LambdaGEM-11, Promega) library of a Norwegian landrace pig was screened at intermediate stringency as described previously (Tornsten et al 1998) with the human NPY4R and mouse NPY6R clones as probes. A partial porcine NPY1R cDNA clone (provided by Mona Byström and Jonas Ekstrand, Astra Hässle, Umeå, Sweden) was used in high stringency (60°C) hybridizations and washes with ‫ן5.0‬ SSC, 0.1% SDS.…”

Section: Screening Of Porcine Phage and Bac Librariesmentioning

confidence: 99%

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Evolution of the Neuropeptide Y Receptor Family: Gene and Chromosome Duplications Deduced from the Cloning and Mapping of the Five Receptor Subtype Genes in Pig

Wraith¹,

Törnsten²,

Chardon³

et al. 2000

Genome Res.

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Neuropeptide Y (NPY) receptors mediate a variety of physiological responses including feeding and vasoconstriction. To investigate the evolutionary events that have generated this receptor family, we have sequenced and determined the chromosomal localizations of all five presently known mammalian NPY receptor subtype genes in the domestic pig, Sus scrofa (SSC). The orthologs of the Y 1 and Y 2 subtypes display high amino acid sequence identities between pig, human, and mouse (92%-94%), whereas the Y 4 , Y 5 , and y 6 subtypes display lower identities (76%-87%). The lower identity of Y 5 is due to high sequence divergence in the large third intracellular loop. The NPY1R, NPY2R, and NPY5R receptor genes were localized to SSC8, the NPY4R to SSC14, and NPY6R to SSC2. Our comparisons strongly suggest that the tight cluster of NPY1R, NPY2R, and NPY5R on human chromosome 4 (HSA4) represents the ancestral configuration, whereas the porcine cluster has been split by two inversions on SSC8. These 3 genes, along with adjacent genes from 14 other gene families, form a cluster on HSA4 with extensive similarities to a cluster on HSA5, where NPY6R and >13 other paralogs reside, as well as another large cluster on HSA10 that includes NPY4R. Thus, these gene families have expanded through large-scale duplications. The sequence comparisons show that the NPY receptor triplet NPY1R-NPY2R-NPY5R existed before these large-scale duplications.

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Section: Localization Of Npy Receptorssupporting

confidence: 60%

Section: Cloning and Sequence Analysis Of Porcine Npy Receptor Genesmentioning

confidence: 99%

Section: Fish Mappingmentioning

confidence: 99%

Section: Screening Of Porcine Phage and Bac Librariesmentioning

confidence: 99%

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Evolution of the Neuropeptide Y Receptor Family: Gene and Chromosome Duplications Deduced from the Cloning and Mapping of the Five Receptor Subtype Genes in Pig

Wraith¹,

Törnsten²,

Chardon³

et al. 2000

Genome Res.

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“…(1995) near interleukin 2 (IL2) and maps to HSA 4q28, further supporting the detected rearrangement. However, a recent assignment of neuropeptide Y receptor Y5 (NPY5) to SSC 8p1·2 ( Törnsten et al . 1998 ) could indicate a microrearrangement in gene order if the authors prediction of this gene’s location in the human genome is correct (HSA 4q31–32).…”

Section: Sequence Information Used To Amplify Segments Of Genes From mentioning

confidence: 99%

Mapping four genes from human chromosome 4 to porcine chromosome 8 further develops the comparative map for an economically important chromosome of the swine genome

Rohrer

1999

Animal Genetics

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Because porcine chromosome (SSC) 8 has become the focal point of many efforts aimed at identifying quantitative trait loci affecting ovulation rate, genes distributed across human chromosome (HSA) 4 were physically mapped in the pig. A more refined comparative map of this region for these two species was produced. In this study, four genes were selected based on their location in the human genome, the availability of nucleotide sequence and their genomic organization. The genes selected were fibroblast growth factor basic (FGF2; HSA 4q25-27), gonadotropin releasing hormone receptor (GNRHR; HSA 4q13), phosphodiesterase 6 B (PDE6B; HSA 4p16.3) and aminopeptidase S (PEPS; HSA 4p11-q12). Genomic libraries were screened via PCR and clones were physically assigned using fluorescence in situ hybridization (FISH). These four genes from HSA 4 were physically mapped to SSC 8p2.3 (PDE6B), 8p1.1 (PEPS), 8q1.1-1.2 (GNRHR) and 8q2.2-2.4 (FGF2). These assignments provide additional benchmarks for the comparative map and help define the level of gene order conserved between HSA 4 and SSC 8.

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FISH mapping of the porcine NPY5 gene to Chromosome 8p11

Cited by 2 publications

References 9 publications

Evolution of the Neuropeptide Y Receptor Family: Gene and Chromosome Duplications Deduced from the Cloning and Mapping of the Five Receptor Subtype Genes in Pig

Evolution of the Neuropeptide Y Receptor Family: Gene and Chromosome Duplications Deduced from the Cloning and Mapping of the Five Receptor Subtype Genes in Pig

Mapping four genes from human chromosome 4 to porcine chromosome 8 further develops the comparative map for an economically important chromosome of the swine genome

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