FISH mapping of 5S rRNA genes in chromosomes of North American abalone species, Haliotis rufescens and H. fulgens

Hernández-Ibarra, Norma K.; Ibarra, Ana M.; Cruz, Pedro; Leitch, Andrew R.

doi:10.1016/j.aquaculture.2007.07.086

Cited by 3 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In reference to abalones, although there have been many chromosomal studies on Haliotis , most of them presented only basic karyological data for species such as the number and structure of the chromosomes present [9–15]. To date there are only a few available karyological studies using FISH, such as location of ribosomal DNA(rDNA) in H. rufescens , analysis of telomeres and investigating the presence of (GATA)n microsatellites[16–19]. …”

Section: Introductionmentioning

confidence: 99%

Cytogenetic Analysis and Chromosomal Characteristics of the Polymorphic 18S rDNA of Haliotis discus hannai from Fujian, China

et al. 2015

View full text Add to dashboard Cite

We report on novel chromosomal characteristics of Haliotis discus hannai from a breeding population at Fujian, China. The karyotypes of H. discus hannai we obtained from an abalone farm include a common type 2n = 36 = 10M + 8SM (82%) and two rare types 2n = 36 = 11M + 7SM (14%) and 2n = 36 = 10M + 7SM + 1ST (4%). The results of silver staining showed that the NORs of H. discus hannai were usually located terminally on the long arms of chromosome pairs 14 and 17, NORs were also sometimes located terminally on the short arms of other chromosomes, either metacentric or submetacentric pairs. The number of Ag-nucleoli ranged from 2 to 8, and the mean number was 3.61 ± 0.93. Among the scored interphase cells, 41% had 3 detectable nucleoli and 37% had 4 nucleoli. The 18S rDNA FISH result is the first report of the location of 18S rDNA genes in H. discus hannai. The 18S rDNA locations were highly polymorphic in this species. Copies of the gene were observed in the terminal of long or/and short arms of submetacentric or/and metacentric chromosomes. Using FISH with probe for vertebrate-like telomeric sequences (CCCTAA)3 displayed positive green FITC signals at telomere regions of all analyzed chromosome types. We found about 7% of chromosomes had breaks in prophase. A special form of nucleolus not previously described from H. discus hannai was observed in some interphase cells. It consists of many small silver-stained nucleoli gathered together to form a larger nucleolus and may correspond to prenucleolar bodies.

show abstract

Section: Introductionmentioning

confidence: 99%

Cytogenetic Analysis and Chromosomal Characteristics of the Polymorphic 18S rDNA of Haliotis discus hannai from Fujian, China

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The distribution of 5S rDNA of diploid H. discus hannai was similar to that of H . rufescens and H. fulgen [32]. While most studies have found the distribution of 5S rDNA chromosome loci to be very conserved [33‒35], but it is also found that some bivalves like Venerupis corrugate [36], Polititapes aureus [37], have the opposite distribution pattern.…”

Section: Discussionmentioning

confidence: 99%

Comparative Cytogenetic Analysis of Diploid and Triploid Pacific Abalone, <i>Haliotis discus hannai</i>

Zhang,

Wang,

et al. 2023

Cytogenet Genome Res

View full text Add to dashboard Cite

Cytogenetic analysis of triploid Haliotis discus hannai larvae (produced by chemical treatment) and its diploids were performed. The results showed that triploid H. discus hannai had a chromosome number of 3n = 54, consisting of 30 metacentric (m) and 24 submetacentric (sm) chromosomes, while the diploids had a chromosome number of 2n = 36, consisting of 20 metacentric (m) and 16 submetacentric (sm) chromosomes. Notably, both triploids and diploids displayed variation in the number of NORs and/or their diameter. The average number of NORs was significantly higher in triploids than in diploids (P < 0.05), while there was no significant difference in the average diameter of NORs between the two groups (P > 0.05). Additionally, 5S rDNA localization to 3 submetacentric chromosomes was observed in triploids, compared to 2 submetacentric chromosomes in diploids. The number of 18S rDNA sites displayed positional conservancy and quantitative variability in both diploids and triploids. Specifically, 18S rDNA was found at the end of the chromosome in both groups, with triploids exhibiting a significantly higher number of loci than diploids (P < 0.01). This study provides valuable insights into the cytogenetic characteristics of triploid H. discus hannai, which could facilitate further research on the stability of the chromosome set in this species.

show abstract

“…Although lots of studies have given new insight into the phylogenetic status of Haliotidae, the mechanism involve in chromosomal variability are still unknown. The cytogenetic research of Haliotidae mainly focused on the traditional karyotype analysis, chromosome banding and chromosome localization of rDNA (Okumura et al, 1995;Hernańdez-Ibarra et al, 2007;Wang et al, 2015). But the chromosome evolution in Haliotidae may be more dynamic beyond the previous knowledge.…”

Section: Discussionmentioning

confidence: 99%

Development and applications of chromosome-specific BAC-FISH probes in Pacific abalone (Haliotis discus hannai)

Zhang,

Wang,

Huang

et al. 2023

Front. Mar. Sci.

View full text Add to dashboard Cite

Pacific abalone (Haliotis discus hannai) is an economically important marine shellfish for aquaculture and is distributed throughout eastern Asia. Although a lot of genetic breeding work has been carried out, chromosome identification in abalone is still a challenging task. Here, we developed a set of BACs to be chromosome-specific probes in Pacific abalone, and to study chromosome evolution in the related species. Through BAC paired-end sequencing and sequence alignment, we were able to in silico anchor 168 BACs onto 18 pseudochromosomes of Pacific abalone genome. After selecting 42 BACs that contained DNA inserts with minimal repetitive sequences, we validated them through PCR and Fluorescence in situ hybridization (FISH) test. As a result, We obtained specific FISH signals for 26 clones on the chromosomes of Pacific abalone with at least one BAC mapped per chromosome. We also applied the chromosome-specific BAC-FISH probes to a close relative of Pacific abalone, Xishi abalone (H. gigantea), which revealed that chromosome 13 and 15 between the two species underwent a chromosomes rearrangement event. This study provides the first set of chromosome-specific probes for the family Haliotidae, which can serve as an important tool for future cytogenetics and genomics research.

show abstract

FISH mapping of 5S rRNA genes in chromosomes of North American abalone species, Haliotis rufescens and H. fulgens

Cited by 3 publications

References 0 publications

Cytogenetic Analysis and Chromosomal Characteristics of the Polymorphic 18S rDNA of Haliotis discus hannai from Fujian, China

Cytogenetic Analysis and Chromosomal Characteristics of the Polymorphic 18S rDNA of Haliotis discus hannai from Fujian, China

Comparative Cytogenetic Analysis of Diploid and Triploid Pacific Abalone, <i>Haliotis discus hannai</i>

Development and applications of chromosome-specific BAC-FISH probes in Pacific abalone (Haliotis discus hannai)

Contact Info

Product

Resources

About