Fish-isolated Naegleria strains and their phylogeny inferred from  ITS and SSU rDNA sequences

Dyková, Iva; Pecková, Hana; Fiala, Ivan; Dvořáková, Helena

doi:10.14411/fp.2006.023

Cited by 15 publications

(10 citation statements)

References 23 publications

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“…of SSU rDNA sequences deposited in GenBank are summarized in Table 1. The Naegleria strain (GERK) isolated from rainbow trout sampled in farm K (from a fish with histologically proven infection with amoeba trophozoites) was found to be closely related to Naegleria strains isolated previously from gills of roach Rutilus rutilus (L.) and skin of wels catfish Silurus glanis L. (Dyková et al 2006). The generic diagnosis of the isolated amoeba strains revealed a broad spectrum of possible agents of gill lesions, but did not allow relating them to amoebae found in histological material.…”

Section: Amoebae Isolated From Ngd-affected Gillsmentioning

confidence: 82%

New data on aetiology of nodular gill disease in rainbow trout, Oncorhynchus mykiss

Dyková¹,

Kostka²,

Wortberg³

et al. 2010

FOLIA PARASIT

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Abstract:We studied amoebae associated with nodular gill disease (NGD) outbreaks in rainbow trout Oncorhynchus mykiss (Walbaum) in fish farms in South-Western Germany. Gills of 12 diseased rainbow trout were examined in fresh, by isolation attempts, histologically and using in situ hybridisation (ISH). A total of nine amoeba strains of the genera Acanthamoeba (1), Hartmannella (2), Naegleria (1), Protacanthamoeba (1) and Vannella (4) were isolated and determined using light microscopical, ultrastructural and molecular methods. Specific molecular probes designed from the SSU rDNA sequences of individual amoeba strains were used for non-radioactive ISH in histological sections. Association of Naegleria sp. with NGD and a direct ISH proof of Naegleria trophozoites attached to hyperplastic gill epithelium are novel findings, expanding the number of possible agents of NGD and supporting the hypothesis on multicausal aetiology of this disease.

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Section: Amoebae Isolated From Ngd-affected Gillsmentioning

confidence: 82%

New data on aetiology of nodular gill disease in rainbow trout, Oncorhynchus mykiss

Dyková¹,

Kostka²,

Wortberg³

et al. 2010

FOLIA PARASIT

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“…The phylogenetic reconstruction of the SSU rDNA sequence data using methods of maximum parsimony (MP), maximum likelihood (ML) and Bayesian inference (BI) followed the practice applied in previous studies (Dyková et al 2005a(Dyková et al , 2006. MP (heuristic search with three bisection-reconnection branch swapping; gaps as missing data; transition/transversion (Ts:Tv) ratio=1:2; bootstrap statistical support with 1 000 replications) and ML (GTR+I+Γ model of evolution; bootstrap statistical support with 500 replications) analyses were performed in the program package PAUP* version 4.0b10 (Swofford 2001).…”

Section: Methodsmentioning

confidence: 99%

Didymium-like myxogastrids (class Mycetozoa) as endocommensals of sea urchins (Sphaerechinus granularis)

Dyková¹,

Lom²,

Dvořáková³

et al. 2007

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Abstract. This paper sums up the results of light microscopical, ultrastructural and molecular studies of five strains of amoeboid organisms isolated as endocommensals from coelomic fluid of sea urchins, Sphaerechinus granularis (Lamarck), collected in the Adriatic Sea. The organisms are reported as Didymium-like myxogastrids. Of the life-cycle stages, the attached amoeboids, flagellated trophozoites, cysts and biflagellated swarmers are described. Formation of fruiting bodies was not observed. Although phylogenetic analyses of SSU rDNA sequences indicated a close relationship with Hyperamoeba dachnaya, our sea-urchin strains have not been assigned to the genus Hyperamoeba Alexeieff, 1923. The presence of either one or two flagella reported in phylogenetically closely related organisms and mutually distant phylogenetic positions of strains declared as representatives of the genus Hyperamoeba justify our approach. Data obtained in this study may be useful in future analyses of relationships of the genera Didymium, Hyperamoeba, Physarum and Pseudodidymium as well as in higher-order phylogeny of Myxogastrea.The interest in amoebic diseases of invertebrates recently renewed by outbreaks associated with mass mortalities in American lobsters in Long Island Sound, USA (Mullen et al. 2004(Mullen et al. , 2005, as well as continuing research of Amoebic Gill Disease (AGD) in farmed fish, inspired isolation attempts focused on Paramoeba/ Neoparamoeba spp. as potential agents of infections in invertebrates including sea urchins. Mass mortalities of echinoids attributed to Paramoeba invadens Jones, 1985 infections were reported in the green sea urchin Strongylocentrotus droebachiensis along the Atlantic coast of Nova Scotia, Canada, between 1980 and 1983(Jones 1985.Since the number of amoeboid organisms isolated from invertebrates and stored in culture collections is rather limited, we have characterized several strains isolated from the first set of sea urchins we have sampled thus far. None of the isolated strains could be assigned to Paramoeba/Neoparamoeba species searched for; most of isolated strains were mutually similar and interesting enough to be presented in this study. MATERIALS AND METHODSFive strains of amoeboid organisms presented in the study were isolated from 5 out of 34 sea urchins Sphaerechinus granularis (Lamarck, 1816) screened for the presence of amphizoic amoebae in their coelomic fluid. Strains were denominated as follows: ECH1, ECH14, ECH43, ECH 49, and ECH54. Sea urchins were collected in the Adriatic Sea, in the nearshore area of the Brać Island (Croatia), in June 2005. Sampling of sea urchins was performed using sterile disposable "self-filling" syringes with needles of 23 gauge designed for bleeding in pigs. Modified malt and yeast extract seawater agar (MY75S in the Catalogue of the UK National Culture Collection 2001) was used for isolation attempts as well as for subculturing of primary isolates, strains and clones. The strength of artificial seawater (Sea salts Sigma S9883) was lowered to 30%...

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“…Heteroloboseans were found also in the gut of animals (e.g., Tetramitus spp., Paravahlkampfia ustiana, Percolomonas sulcatus) and gills, skin, and internal organs of fish (Naegleria spp.) (e.g., Brugerolle & Simpson, 2004;Dyková et al, 2001Dyková et al, , 2006Schuster et al, 2003).…”

Section: Ecology Of Heteroloboseamentioning

confidence: 99%

“…Despite strains of Naegleria fowleri are even more variable, they are considered to be conspecific instead of belonging to several different species (De Jonckheere, 2004, 2011. Dyková et al (2006) even showed that there exists an intragenomic polymorphism in Naegleria clarki. All these examples show that the ITS region should be no longer used for new species descriptions until the problem of species concept and intraspecific polymorphism within Heterolobosea is settled.…”

Section: Species Concept Of Heteroloboseamentioning

confidence: 99%

Diversity of Heterolobosea

Pánek¹,

Čepička²

2012

Genetic Diversity in Microorganisms

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Fish-isolated Naegleria strains and their phylogeny inferred from ITS and SSU rDNA sequences

Cited by 15 publications

References 23 publications

New data on aetiology of nodular gill disease in rainbow trout, Oncorhynchus mykiss

New data on aetiology of nodular gill disease in rainbow trout, Oncorhynchus mykiss

Didymium-like myxogastrids (class Mycetozoa) as endocommensals of sea urchins (Sphaerechinus granularis)

Diversity of Heterolobosea

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