Fish Intersexuality as Indicator of Environmental Stress

Bortone, Stephen A.; Davis, William P.

doi:10.2307/1312253

Cited by 105 publications

(48 citation statements)

References 37 publications

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“…If eggs are laid during the months of heaviest rainfall, the effects of estrogens in discharges should be at a minimum, and perhaps the main problem may therefore lie with the gonadal maturation of the adults prior to spawning. Parallel situations have been reported in Florida [46], where populations of three species of female poecilid fish had been masculinized by compounds present in kraft mill effluents acting as endocrine disruptors. No data for effects at the population level were available, but the masculinization did not progress once fish were transferred to clean water, and unpublished data suggest that these fish were then capable of producing viable offspring.…”

Section: Implications For Wild Fishmentioning

confidence: 57%

A survey of estrogenic activity in United Kingdom inland waters

Harries¹,

Sheahan²,

Matthiessen³

et al. 1996

Enviro Toxic and Chemistry

364

167

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Abstract-Vitellogenin, a yolk-precursor protein normally found only in the blood plasma of sexually mature female teleosts and other egg-laying vertebrates, was used as an indicator of exposure of male rainbow trout (Oncorhynchus mykiss) to exogenous estrogens. Vitellogenin concentrations were measured using a specific radioimmunoassay for trout. Cages containing adult male trout were placed at the points of discharge and at varying distances downstream of treated sewage effluent outfalls into the River Lea (UK) during the summer and winter months of 1992. After 3 weeks exposure at the majority of sites, fish held up to 15 km downstream of inputs showed an increase in plasma vitellogenin concentration, with statistically significant elevations up to 4.5 km downstream. A repeat survey in November 1992 below Harpenden sewage treatment works showed that the only two stations to give a significant response were 3 m and 1.6 km downstream of the outfall. This reduced effect compared to the first survey is thought to be due to dilution of both the influent sewage to the treatment works and of the river water itself by increased rainfall, the overall increase in dilution being approx. 36%. Trout were also placed in 15 raw water storage reservoirs in southeast England during the summer of 1993 for an exposure period of 6 weeks. No biologically significant increases in plasma vitellogenin concentration were observed in any of the reservoirs.

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Section: Implications For Wild Fishmentioning

confidence: 57%

A survey of estrogenic activity in United Kingdom inland waters

Harries¹,

Sheahan²,

Matthiessen³

et al. 1996

Enviro Toxic and Chemistry

364

167

View full text Add to dashboard Cite

show abstract

“…In the present experiment the "sh were only starting the maturation process and it is unknown whether the phytosterols were accumulating elsewhere in fat-rich tissue. It is also unknown whether the "sh were transforming the original compounds to metabolites of steroid character themselves or if the phytosterols were microbiologically transformed during the external e%uent treatment process (Denton et al, 1985;Bortone and Davis, 1994). It is known that sitosterol is microbiologically transformed to androstenedione, which is an intermediate compound in the synthesis of estradiol and testosterone.…”

Section: Discussionmentioning

confidence: 99%

Effects of Pulp Mill Effluents and Restricted Diet on Growth and Physiology of Rainbow Trout (Oncorhynchus mykiss)

Mattsson

Lehtinen²,

Tana³

et al. 2001

Ecotoxicology and Environmental Safety

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“…When fish are exposed to estrogenic compounds, the yolk protein vitellogenin is induced (Sumpter and Jobling, 1995;Bjerregaard et al, 1998;Schultz et al, 2001), and is an especially sensitive biomarker for this class of endocrine disrupting compounds. Adverse impacts to wild fish populations exposed to estrogenic compounds have been documented, which include altered sexual phenotype, altered sex ratios, decreased fecundity, and impaired embryonic development (Bortone and Davis, 1994,Parks et al, 2001,Schultz et al, 2003.…”

Section: Introductionmentioning

confidence: 99%

Temporal changes in gene expression in rainbow trout exposed to ethynyl estradiol

Hook

Skillman

Small

et al. 2007

Comparative Biochemistry and Physiology Part C: Toxicology &

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We examined changes in the genomic response during continuous exposure to the xenoestrogen ethynylestradiol. Isogenic rainbow trout Oncorhynchus mykiss were exposed to nominal concentrations of 100 ng/L ethynyl estradiol (EE2) for a period of three weeks. At fixed time points within the exposure, fish were euthanized, livers harvested and RNA extracted. Fluorescently labeled cDNA were generated and hybridized against a commercially available Salmonid array (GRASP project, University of Victoria, Canada) spotted with 16,000 cDNAs. The slides were scanned to measure abundance of a given transcript in each sample relative to controls. Data were analyzed via Genespring (Silicon Genetics) to identify a list of up and down regulated genes, and to determine gene clustering patterns that can be used as "expression signatures". Gene ontology was determined using the annotation available from the GRASP website. Our analysis indicates each exposure time period generated specific gene expression profiles. Changes in gene expression were best understood by grouping genes by their gene expression profiles rather than examining fold change at a particular time point. Many of the genes commonly used as biomarkers of exposure to xenoestrogens were not induced initially and did not have gene expression profiles typical of the majority of genes with altered expression.

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Fish Intersexuality as Indicator of Environmental Stress

Cited by 105 publications

References 37 publications

A survey of estrogenic activity in United Kingdom inland waters

A survey of estrogenic activity in United Kingdom inland waters

Effects of Pulp Mill Effluents and Restricted Diet on Growth and Physiology of Rainbow Trout (Oncorhynchus mykiss)

Temporal changes in gene expression in rainbow trout exposed to ethynyl estradiol

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