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Cited by 37 publications
(34 citation statements)
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“…Fluorescence in situ hybridization (FISH) was performed under high stringency conditions on metaphase chromosome spreads, as described in Yano et al (2017a) . The chromosome slides were incubated with RNAse (10 μg/mL) for 1 h at 37°C in a wet chamber and then washed for 5 min in 1x PBS and incubated with pepsin 0,005% for 10 min at room temperature.…”
Section: Methodsmentioning
confidence: 99%
“…Fluorescence in situ hybridization (FISH) was performed under high stringency conditions on metaphase chromosome spreads, as described in Yano et al (2017a) . The chromosome slides were incubated with RNAse (10 μg/mL) for 1 h at 37°C in a wet chamber and then washed for 5 min in 1x PBS and incubated with pepsin 0,005% for 10 min at room temperature.…”
Section: Methodsmentioning
confidence: 99%
“…Fluorescence in situ hybridization (FISH) experiments were performed as described in Yano et al (2017) [ 25 ], with slight modifications. The experiment was performed under high stringency conditions on mitotic chromosome spreads.…”
Section: Methodsmentioning
confidence: 99%
“…Fluorescence in situ hybridization (FISH) was performed according to Yano et al [2017], using tandemly repeated genes coding for 5S and 18S rRNA as probes, containing approximately 200 and 1,400 bp, respectively. Probes were obtained by PCR from the nuclear DNA of Rachycentron canadum (Rachycentridae), employing A 5 ′ -TACGCCCGATCTCGTCCG ATC-3 ′ and B 5 ′ -CAG-GCTGGTATGGCCGTAAGC-3 ′ primers for 5S rDNA [Pendás et al, 1994], and NS1 5 ′ -GTAGTCATATGCTTGTCTC-3 ′ and NS8 5 ′ -TCCGCAGGTTCACCTACGGA-3 ′ primers for 18S rDNA [White et al, 1990].…”
Section: Chromosome Preparations and Cytogenetic Analysesmentioning
confidence: 99%